Abstract
A mutation (Acc1-S2) in the structural gene for maize (Zea mays L.) acetyl-coenzyme A carboxylase (ACCase) that significantly reduces sethoxydim inhibition of leaf ACCase activity was used to investigate the gene-enzyme relationship regulating ACCase activity during oil deposition in developing kernels. Mutant embryo and endosperm ACCase activities were more than 600-fold less sensitive to sethoxydim inhibition than ACCase in wild-type kernel tissues. Moreover, in vitro cultured mutant kernels developed normally in the presence of sethoxydim concentrations that inhibited wild-type kernel development. The results indicate that the Acc1-encoded ACCase accounts for the majority of ACCase activity in developing maize kernels, suggesting that Acc1-encoded ACCase functions not only during membrane biogenesis in leaves but is also the predominant form of ACCase involved in storage lipid biosynthesis in maize embryos.
Full Text
The Full Text of this article is available as a PDF (500.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Burton J. D., Gronwald J. W., Somers D. A., Connelly J. A., Gengenbach B. G., Wyse D. L. Inhibition of plant acetyl-coenzyme A carboxylase by the herbicides sethoxydim and haloxyfop. Biochem Biophys Res Commun. 1987 Nov 13;148(3):1039–1044. doi: 10.1016/s0006-291x(87)80236-x. [DOI] [PubMed] [Google Scholar]
- Egli M. A., Gengenbach B. G., Gronwald J. W., Somers D. A., Wyse D. L. Characterization of Maize Acetyl-Coenzyme A Carboxylase. Plant Physiol. 1993 Feb;101(2):499–506. doi: 10.1104/pp.101.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- López-Casillas F., Ponce-Castañeda M. V., Kim K. H. In vivo regulation of the activity of the two promoters of the rat acetyl coenzyme-A carboxylase gene. Endocrinology. 1991 Aug;129(2):1049–1058. doi: 10.1210/endo-129-2-1049. [DOI] [PubMed] [Google Scholar]
- Somerville C., Browse J. Plant lipids: metabolism, mutants, and membranes. Science. 1991 Apr 5;252(5002):80–87. doi: 10.1126/science.252.5002.80. [DOI] [PubMed] [Google Scholar]