Abstract
A heterologous probe encoding phenylalanine ammonia-lyase (PAL) was used to identify PAL clones in cDNA libraries made with RNA from young leaf tissue of two Populus deltoides x P. trichocarpa F1 hybrid clones. Sequence analysis of a 2.4-kb cDNA confirmed its identity as a full-length PAl clone. The predicted amino acid sequence is conserved in comparison with that of PAL genes from several other plants. Southern blot analysis of popular genomic DNA from parental and hybrid individuals, restriction site polymorphism in PAL cDNA clones, and sequence heterogeneity in the 3' ends of several cDNA clones suggested that PAL is encoded by at least two genes that can be distinguished by HindIII restriction site polymorphisms. Clones containing each type of PAL gene were isolated from a poplar genomic library. Analysis of the segregation of PAL-specific HindIII restriction fragment-length polymorphisms demonstrated the existence of two independently segregating PAL loci, one of which was mapped to a linkage group of the poplar genetic map. Developmentally regulated PAL expression in poplar was analyzed using RNA blots. Highest expression was observed in young stems, apical buds, and young leaves. Expression was lower in older stems and undetectable in mature leaves. Cellular localization of PAL expression by in situ hybridization showed very high levels of expression in subepidermal cells of leaves early during leaf development. In stems and petioles, expression was associated with subepidermal cells and vascular tissues.
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- Bevan M., Shufflebottom D., Edwards K., Jefferson R., Schuch W. Tissue- and cell-specific activity of a phenylalanine ammonia-lyase promoter in transgenic plants. EMBO J. 1989 Jul;8(7):1899–1906. doi: 10.1002/j.1460-2075.1989.tb03592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campbell M. M., Ellis B. E. Fungal Elicitor-Mediated Responses in Pine Cell Cultures : III. Purification and Characterization of Phenylalanine Ammonia-Lyase. Plant Physiol. 1992 Jan;98(1):62–70. doi: 10.1104/pp.98.1.62. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Block M. Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens-Mediated Transformation of Hybrid Aspen and Poplar Clones. Plant Physiol. 1990 Jul;93(3):1110–1116. doi: 10.1104/pp.93.3.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fritzemeier K. H., Cretin C., Kombrink E., Rohwer F., Taylor J., Scheel D., Hahlbrock K. Transient Induction of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase mRNAs in Potato Leaves Infected with Virulent or Avirulent Races of Phytophthora infestans. Plant Physiol. 1987 Sep;85(1):34–41. doi: 10.1104/pp.85.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gowri G., Paiva N. L., Dixon R. A. Stress responses in alfalfa (Medicago sativa L.) 12. Sequence analysis of phenylalanine ammonia-lyase (PAL) cDNA clones and appearance of PAL transcripts in elicitor-treated cell cultures and developing plants. Plant Mol Biol. 1991 Sep;17(3):415–429. doi: 10.1007/BF00040636. [DOI] [PubMed] [Google Scholar]
- Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
- Lois R., Dietrich A., Hahlbrock K., Schulz W. A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J. 1989 Jun;8(6):1641–1648. doi: 10.1002/j.1460-2075.1989.tb03554.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lois R., Hahlbrock K. Differential wound activation of members of the phenylalanine ammonia-lyase and 4-coumarate:CoA ligase gene families in various organs of parsley plants. Z Naturforsch C. 1992 Jan-Feb;47(1-2):90–94. doi: 10.1515/znc-1992-1-216. [DOI] [PubMed] [Google Scholar]
- Ohl S., Hedrick S. A., Chory J., Lamb C. J. Functional properties of a phenylalanine ammonia-lyase promoter from Arabidopsis. Plant Cell. 1990 Sep;2(9):837–848. doi: 10.1105/tpc.2.9.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parsons T. J., Bradshaw H. D., Jr, Gordon M. P. Systemic accumulation of specific mRNAs in response to wounding in poplar trees. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7895–7899. doi: 10.1073/pnas.86.20.7895. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reinold S., Hauffe K. D., Douglas C. J. Tobacco and Parsley 4-Coumarate:Coenzyme A Ligase Genes Are Temporally and Spatially Regulated in a Cell Type-Specific Manner during Tobacco Flower Development. Plant Physiol. 1993 Feb;101(2):373–383. doi: 10.1104/pp.101.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmelzer E., Kruger-Lebus S., Hahlbrock K. Temporal and Spatial Patterns of Gene Expression around Sites of Attempted Fungal Infection in Parsley Leaves. Plant Cell. 1989 Oct;1(10):993–1001. doi: 10.1105/tpc.1.10.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whetten R. W., Sederoff R. R. Phenylalanine ammonia-lyase from loblolly pine : purification of the enzyme and isolation of complementary DNA clones. Plant Physiol. 1992 Jan;98(1):380–386. doi: 10.1104/pp.98.1.380. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Sá M. M., Subramaniam R., Williams F. E., Douglas C. J. Rapid Activation of Phenylpropanoid Metabolism in Elicitor-Treated Hybrid Poplar (Populus trichocarpa Torr. & Gray x Populus deltoides Marsh) Suspension-Cultured Cells. Plant Physiol. 1992 Feb;98(2):728–737. doi: 10.1104/pp.98.2.728. [DOI] [PMC free article] [PubMed] [Google Scholar]