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. 1973 Dec 15;4(5893):640–643. doi: 10.1136/bmj.4.5893.640

Variation in Plasma Calcium with Induced Changes in Plasma Specific Gravity, Total Protein, and Albumin

E M Berry, M M Gupta, S J Turner, R R Burns
PMCID: PMC1587667  PMID: 4758543

Abstract

The relationship of plasma calcium levels to changes in plasma specific gravity, total protein, and albumin induced by venous stasis was investigated. Factors were derived for adjusting calcium results to offset the effects of variation in protein concentration and thus to make them of increased discriminatory value to the clinician. The validity of an existing specific gravity correction has been substantiated, but a more exact adjustment of 0·23 mg/100 ml of calcium for every 0·001 change in specific gravity is proposed. We recommend for automated laboratories that the factor based on albumin be used: 0·09 mg/100 ml of calcium should be subtracted from the total calcium value for every increase of 0·1 g in albumin above 4·6 g/100 ml, and a corresponding addition should be made for values of albumin below 4·6 g/100 ml.

Using a calcium specific electrode, it has been shown that the ionized calcium concentration does not alter with prolonged venous stasis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FANCONI A., ROSE G. A. The ionized, complexed, and protein-bound fractions of calcium in plasma; an investigation of patients with various diseases which affect calcium metabolism, with an additional study of the role of calcium ions in the prevention of tetany. Q J Med. 1958 Oct;27(108):463–494. [PubMed] [Google Scholar]
  2. Gutman A. B., Gutman E. B. RELATION OF SERUM CALCIUM TO SERUM ALBUMIN AND GLOBULINS. J Clin Invest. 1937 Nov;16(6):903–919. doi: 10.1172/JCI100917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HANNA E. A., NICHOLAS H. O., CHAMBERLIN J. A. NOMOGRAM FOR ESTIMATING DIFFUSIBLE SERUM CALCIUM. Clin Chem. 1964 Mar;10:235–240. [PubMed] [Google Scholar]
  4. HOPKINS T., HOWARD J. E., EISENBERG H. Ultrafiltration studies on calcium and phosphorus in human serum. Bull Johns Hopkins Hosp. 1952 Jul;91(1):1–21. [PubMed] [Google Scholar]
  5. LOKEN H. F., HAVEL R. J., GORDAN G. S., WHITTINGTON S. L. Ultracentrifugal analysis of protein-bound and free calcium in human serum. J Biol Chem. 1960 Dec;235:3654–3658. [PubMed] [Google Scholar]
  6. Moore E. W. Studies with ion exchange calcium electrodes. 3. The state of serum calcium in patients with cirrhosis. Gastroenterology. 1971 Jan;60(1):43–54. [PubMed] [Google Scholar]
  7. Orrell D. H. Albumin as an aid to the interpretation of serum calcium. Clin Chim Acta. 1971 Dec;35(2):483–489. doi: 10.1016/0009-8981(71)90224-5. [DOI] [PubMed] [Google Scholar]
  8. Pedersen K. O. On the cause and degree of intraindividual serum calcium variability. Scand J Clin Lab Invest. 1972 Oct;30(2):191–199. doi: 10.3109/00365517209081110. [DOI] [PubMed] [Google Scholar]
  9. TORIBARA T. Y., TEREPKA A. R., DEWEY P. A. The ultrafiltrable calcium of human serum. I. Ultrafiltration methods and normal values. J Clin Invest. 1957 May;36(5):738–748. doi: 10.1172/JCI103477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. van LEEUWEN A., THOMASSE C. M., KAPTEYN P. C. The determination of protein-bound calcium and magnesium by ultrafiltration-in-vivo with special reference to terminology and method. Clin Chim Acta. 1961 Jul;6:550–565. doi: 10.1016/0009-8981(61)90147-4. [DOI] [PubMed] [Google Scholar]

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