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Plant Physiology logoLink to Plant Physiology
. 1993 May;102(1):269–277. doi: 10.1104/pp.102.1.269

Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A.

A Nagatani 1, J W Reed 1, J Chory 1
PMCID: PMC158772  PMID: 12231818

Abstract

Phytochrome, a red/far-red-light photoreceptor protein of plants, is encoded by a small gene family. Phytochrome A (PHYA), the product of the PHYA gene, is the predominant molecular species of phytochrome in etiolated tissue and has been best characterized biochemically. To define a role for PHYA, we isolated new mutants, designated fre1 (far-red elongated), in Arabidopsis thaliana that were specifically deficient in PHYA spectral activity and protein accumulation. These mutants were identified on the basis of their long hypocotyl phenotype under continuous far-red light. Although the fre1 mutants lacked the hypocotyl response to continuous far-red light, their responses to continuous white light and to end-of-day far-red-light treatments were normal. Thus, PHYA appears to play only a minor role in the regulation of hypocotyl elongation under natural conditions. In contrast, the fre1 mutation affected greening a fre1 mutant was less able than the wild type to deetiolate after growth in the dark. However, the potentiation effect of a red-light pulse on accumulation of chlorophyll was not changed significantly in the fre1 mutants. Thus, the function of PHYA might be highly specialized and restricted to certain phases of Arabidopsis development.

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Selected References

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  1. Childs K. L., Pratt L. H., Morgan P. W. Genetic Regulation of Development in Sorghum bicolor: VI. The ma(3) Allele Results in Abnormal Phytochrome Physiology. Plant Physiol. 1991 Oct;97(2):714–719. doi: 10.1104/pp.97.2.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chory J., Peto C. A., Ashbaugh M., Saganich R., Pratt L., Ausubel F. Different Roles for Phytochrome in Etiolated and Green Plants Deduced from Characterization of Arabidopsis thaliana Mutants. Plant Cell. 1989 Sep;1(9):867–880. doi: 10.1105/tpc.1.9.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dehesh K., Tepperman J., Christensen A. H., Quail P. H. phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet. 1991 Feb;225(2):305–313. doi: 10.1007/BF00269863. [DOI] [PubMed] [Google Scholar]
  4. Devlin P. F., Rood S. B., Somers D. E., Quail P. H., Whitelam G. C. Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa: ein Mutant Lacks a Detectable Phytochrome B-Like Polypeptide. Plant Physiol. 1992 Nov;100(3):1442–1447. doi: 10.1104/pp.100.3.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elich T. D., Lagarias J. C. Phytochrome Chromophore Biosynthesis : Both 5-Aminolevulinic Acid and Biliverdin Overcome Inhibition by Gabaculine in Etiolated Avena sativa L. Seedlings. Plant Physiol. 1987 Jun;84(2):304–310. doi: 10.1104/pp.84.2.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Furuya M. Molecular properties and biogenesis of phytochrome I and II. Adv Biophys. 1989;25:133–167. doi: 10.1016/0065-227x(89)90006-3. [DOI] [PubMed] [Google Scholar]
  7. Heyer A., Gatz C. Isolation and characterization of a cDNA-clone coding for potato type B phytochrome. Plant Mol Biol. 1992 Nov;20(4):589–600. doi: 10.1007/BF00046444. [DOI] [PubMed] [Google Scholar]
  8. López-Juez E., Nagatani A., Tomizawa K., Deak M., Kern R., Kendrick R. E., Furuya M. The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. Plant Cell. 1992 Mar;4(3):241–251. [PMC free article] [PubMed] [Google Scholar]
  9. Parks B. M., Quail P. H. Phytochrome-Deficient hy1 and hy2 Long Hypocotyl Mutants of Arabidopsis Are Defective in Phytochrome Chromophore Biosynthesis. Plant Cell. 1991 Nov;3(11):1177–1186. doi: 10.1105/tpc.3.11.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parks B. M., Quail P. H. hy8, a new class of arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell. 1993 Jan;5(1):39–48. doi: 10.1105/tpc.5.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reed J. W., Nagpal P., Poole D. S., Furuya M., Chory J. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 1993 Feb;5(2):147–157. doi: 10.1105/tpc.5.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]

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