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. 1993 Jun;102(2):401–408. doi: 10.1104/pp.102.2.401

Genetic and Physiological Analysis of a New Locus in Arabidopsis That Confers Resistance to 1-Aminocyclopropane-1-Carboxylic Acid and Ethylene and Specifically Affects the Ethylene Signal Transduction Pathway.

D Van Der Straeten 1, A Djudzman 1, W Van Caeneghem 1, J Smalle 1, M Van Montagu 1
PMCID: PMC158793  PMID: 12231830

Abstract

A population of M2 seedlings of Arabidopsis thaliana was screened for mutants that were insensitive to the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Several independent lines were obtained and proved insensitive to both ACC and ethylene. Two lines were identified as alleles of a single recessive mutation, designated ain1. Linkage analysis indicated that the ain1 gene is located on chromosome 1, adjacent to the cer5 marker and, therefore, genetically distinct from previously identified ethylene resistance loci. General phenotypic aspects of ain1 mutants were similar to wild type. For both alleles, the level of insensitivity to ethylene at the seedling stage was indistinguishable in terms of elongation growth. In contrast, the gravitropic response of ain1-1 seedlings was slower than that of wild-type and ain1-2 seedlings. At the adult stage, stress responses of mutants were similar to wild type. However, ethylene-induced leaf senescence was delayed in both mutants. In addition, we observed significant interallelic variation in ethylene production rates. Growth inhibition experiments showed that the ain1 mutation does not confer resistance to other hormones. Thus, ain1 most probably affects a step specific for the ethylene signal transduction pathway.

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Selected References

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  1. Bleecker A. B., Estelle M. A., Somerville C., Kende H. Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana. Science. 1988 Aug 26;241(4869):1086–1089. doi: 10.1126/science.241.4869.1086. [DOI] [PubMed] [Google Scholar]
  2. Bowman J. L., Smyth D. R., Meyerowitz E. M. Genetic interactions among floral homeotic genes of Arabidopsis. Development. 1991 May;112(1):1–20. doi: 10.1242/dev.112.1.1. [DOI] [PubMed] [Google Scholar]
  3. Broglie K. E., Biddle P., Cressman R., Broglie R. Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco. Plant Cell. 1989 Jun;1(6):599–607. doi: 10.1105/tpc.1.6.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang C., Bleecker A. B., Kwok S. F., Meyerowitz E. M. Molecular cloning approach for a putative ethylene receptor gene in Arabidopsis. Biochem Soc Trans. 1992 Feb;20(1):73–75. doi: 10.1042/bst0200073. [DOI] [PubMed] [Google Scholar]
  5. Chang C., Bowman J. L., DeJohn A. W., Lander E. S., Meyerowitz E. M. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856–6860. doi: 10.1073/pnas.85.18.6856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giraudat J., Hauge B. M., Valon C., Smalle J., Parcy F., Goodman H. M. Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell. 1992 Oct;4(10):1251–1261. doi: 10.1105/tpc.4.10.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goeschl J. D., Rappaport L., Pratt H. K. Ethylene as a factor regulating the growth of pea epicotyls subjected to physical stress. Plant Physiol. 1966 May;41(5):877–884. doi: 10.1104/pp.41.5.877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grill E., Somerville C. Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet. 1991 May;226(3):484–490. doi: 10.1007/BF00260662. [DOI] [PubMed] [Google Scholar]
  9. Guzmán P., Ecker J. R. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 1990 Jun;2(6):513–523. doi: 10.1105/tpc.2.6.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. He C. J., Morgan P. W., Drew M. C. Enhanced Sensitivity to Ethylene in Nitrogen- or Phosphate-Starved Roots of Zea mays L. during Aerenchyma Formation. Plant Physiol. 1992 Jan;98(1):137–142. doi: 10.1104/pp.98.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jack T., Brockman L. L., Meyerowitz E. M. The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell. 1992 Feb 21;68(4):683–697. doi: 10.1016/0092-8674(92)90144-2. [DOI] [PubMed] [Google Scholar]
  12. Koornneef M., Dellaert L. W., van der Veen J. H. EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res. 1982 Mar;93(1):109–123. doi: 10.1016/0027-5107(82)90129-4. [DOI] [PubMed] [Google Scholar]
  13. Koornneef M., Hanhart C. J., van der Veen J. H. A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet. 1991 Sep;229(1):57–66. doi: 10.1007/BF00264213. [DOI] [PubMed] [Google Scholar]
  14. Lincoln C., Britton J. H., Estelle M. Growth and development of the axr1 mutants of Arabidopsis. Plant Cell. 1990 Nov;2(11):1071–1080. doi: 10.1105/tpc.2.11.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nam H. G., Giraudat J., Den Boer B., Moonan F., Loos WDB., Hauge B. M., Goodman H. M. Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana. Plant Cell. 1989 Jul;1(7):699–705. doi: 10.1105/tpc.1.7.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Raskin I., Kende H. Role of gibberellin in the growth response of submerged deep water rice. Plant Physiol. 1984 Dec;76(4):947–950. doi: 10.1104/pp.76.4.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwarz-Sommer Z., Hue I., Huijser P., Flor P. J., Hansen R., Tetens F., Lönnig W. E., Saedler H., Sommer H. Characterization of the Antirrhinum floral homeotic MADS-box gene deficiens: evidence for DNA binding and autoregulation of its persistent expression throughout flower development. EMBO J. 1992 Jan;11(1):251–263. doi: 10.1002/j.1460-2075.1992.tb05048.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Singh H., LeBowitz J. H., Baldwin A. S., Jr, Sharp P. A. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell. 1988 Feb 12;52(3):415–423. doi: 10.1016/s0092-8674(88)80034-5. [DOI] [PubMed] [Google Scholar]
  19. Sisler E. C. Measurement of ethylene binding in plant tissue. Plant Physiol. 1979 Oct;64(4):538–542. doi: 10.1104/pp.64.4.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Su W., Howell S. H. A Single Genetic Locus, Ckr1, Defines Arabidopsis Mutants in which Root Growth Is Resistant to Low Concentrations of Cytokinin. Plant Physiol. 1992 Aug;99(4):1569–1574. doi: 10.1104/pp.99.4.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Theologis A. One rotten apple spoils the whole bushel: the role of ethylene in fruit ripening. Cell. 1992 Jul 24;70(2):181–184. doi: 10.1016/0092-8674(92)90093-r. [DOI] [PubMed] [Google Scholar]
  22. Van Der Straeten D., Van Montagu M. The molecular basis of ethylene biosynthesis, mode of action, and effects in higher plants. Subcell Biochem. 1991;17:279–326. doi: 10.1007/978-1-4613-9365-8_13. [DOI] [PubMed] [Google Scholar]
  23. Ward E. R., Jen G. C. Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA. Plant Mol Biol. 1990 Apr;14(4):561–568. doi: 10.1007/BF00027501. [DOI] [PubMed] [Google Scholar]
  24. Wilson A. K., Pickett F. B., Turner J. C., Estelle M. A dominant mutation in Arabidopsis confers resistance to auxin, ethylene and abscisic acid. Mol Gen Genet. 1990 Jul;222(2-3):377–383. doi: 10.1007/BF00633843. [DOI] [PubMed] [Google Scholar]

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