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. 1993 Jun;102(2):503–511. doi: 10.1104/pp.102.2.503

Effect of Jasmonic Acid on the Interaction of Barley (Hordeum vulgare L.) with the Powdery Mildew Erysiphe graminis f.sp. hordei.

P Schweizer 1, R Gees 1, E Mosinger 1
PMCID: PMC158805  PMID: 12231839

Abstract

Jasmonic acid or its methyl ester induce de novo synthesis of a number of proteins of mostly unknown function in barley (Hordeum vulgare L.). In a topical spray application, 30 [mu]g of jasmonic acid per plant effectively protected barley against subsequent infection by Erysiphe graminis f.sp. hordei. Examination of jasmonic acid-induced barley proteins revealed the presence of several acid-soluble (pH 2.8) proteins. Two prominent groups of 25 kD and 10 to 12 kD apparent molecular mass were present in the intercellular washing fluid. The set of extracellular, induced proteins showed no similarity to barley pathogenesis-related proteins. An in vivo test against E. graminis revealed no antifungal activity of the extracellular jasmonic acid-induced proteins. Experiments with the transcription inhibitor cordycepin showed no correlation between accumulation of jasmonic acid-induced proteins and protection. The application of jasmonic acid and E. graminis simultaneously resulted in independent extracellular accumulation of both jasmonic acid-induced proteins and of pathogenesis-related proteins. The data suggest that jasmonic acid directly inhibits appressoria differentiation of the fungus, and that it is not involved in the signal transduction mechanism leading to induction of pathogenesis-related proteins.

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Selected References

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  1. Andresen I., Becker W., Schlüter K., Burges J., Parthier B., Apel K. The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare). Plant Mol Biol. 1992 May;19(2):193–204. doi: 10.1007/BF00027341. [DOI] [PubMed] [Google Scholar]
  2. Bohlmann H., Clausen S., Behnke S., Giese H., Hiller C., Reimann-Philipp U., Schrader G., Barkholt V., Apel K. Leaf-specific thionins of barley-a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants. EMBO J. 1988 Jun;7(6):1559–1565. doi: 10.1002/j.1460-2075.1988.tb02980.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chou C. M., Kao C. H. Methyl jasmonate, calcium, and leaf senescence in rice. Plant Physiol. 1992 Aug;99(4):1693–1694. doi: 10.1104/pp.99.4.1693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dittrich H., Kutchan T. M., Zenk M. H. The jasmonate precursor, 12-oxo-phytodienoic acid, induces phytoalexin synthesis in Petroselinum crispum cell cultures. FEBS Lett. 1992 Aug 31;309(1):33–36. doi: 10.1016/0014-5793(92)80733-w. [DOI] [PubMed] [Google Scholar]
  5. Farmer E. E., Johnson R. R., Ryan C. A. Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic Acid. Plant Physiol. 1992 Mar;98(3):995–1002. doi: 10.1104/pp.98.3.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farmer E. E., Ryan C. A. Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors. Plant Cell. 1992 Feb;4(2):129–134. doi: 10.1105/tpc.4.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gundlach H., Müller M. J., Kutchan T. M., Zenk M. H. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2389–2393. doi: 10.1073/pnas.89.6.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hildmann T., Ebneth M., Peña-Cortés H., Sánchez-Serrano J. J., Willmitzer L., Prat S. General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding. Plant Cell. 1992 Sep;4(9):1157–1170. doi: 10.1105/tpc.4.9.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Maslenkova L. T., Miteva T. S., Popova L. P. Changes in the polypeptide patterns of barley seedlings exposed to jasmonic Acid and salinity. Plant Physiol. 1992 Feb;98(2):700–707. doi: 10.1104/pp.98.2.700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mason H. S., Mullet J. E. Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. Plant Cell. 1990 Jun;2(6):569–579. doi: 10.1105/tpc.2.6.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Towbin H., Gordon J. Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods. 1984 Sep 4;72(2):313–340. doi: 10.1016/0022-1759(84)90001-2. [DOI] [PubMed] [Google Scholar]
  13. Ueda J., Kato J. Isolation and Identification of a Senescence-promoting Substance from Wormwood (Artemisia absinthium L.). Plant Physiol. 1980 Aug;66(2):246–249. doi: 10.1104/pp.66.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]

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