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. 1993 Jun;102(2):645–650. doi: 10.1104/pp.102.2.645

A Novel Fungal Protease Expressed in Endophytic Infection of Poa Species.

J T Lindstrom 1, S Sun 1, F C Belanger 1
PMCID: PMC158824  PMID: 12231854

Abstract

The fungus Acremonium typhinum produces a novel endoprotease during symbiotic endophytic infection of the grass, Poa ampla. This protease is unusual because it is highly active in the presence of sodium dodecyl sulfate. The enzyme is a thiol-containing serine protease and is localized to a crude membrane fraction. Similar protease activity has been detected in endophyte-infected Poa autumnalis and Poa sylvestris plants. Expression of this protease may be important in endophytic infection of Poa spp., because similar activity has not been detected in endophyte-infected Festuca arundinacea or Lolium perenne.

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Selected References

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  1. Aoyama A., Chen W. T. A 170-kDa membrane-bound protease is associated with the expression of invasiveness by human malignant melanoma cells. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8296–8300. doi: 10.1073/pnas.87.21.8296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fletcher L. R., Harvey I. C. An association of a Lolium endophyte with ryegrass staggers. N Z Vet J. 1981 Oct;29(10):185–186. doi: 10.1080/00480169.1981.34839. [DOI] [PubMed] [Google Scholar]
  3. Hasnain S., Adeli K., Storer A. C. Purification and characterization of an extracellular thiol-containing serine proteinase from Thermomyces lanuginosus. Biochem Cell Biol. 1992 Feb;70(2):117–122. doi: 10.1139/o92-017. [DOI] [PubMed] [Google Scholar]
  4. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  5. Mizusawa K., Yoshida F. Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability. J Biol Chem. 1973 Jun 25;248(12):4417–4423. [PubMed] [Google Scholar]
  6. Sodeinde O. A., Subrahmanyam Y. V., Stark K., Quan T., Bao Y., Goguen J. D. A surface protease and the invasive character of plague. Science. 1992 Nov 6;258(5084):1004–1007. doi: 10.1126/science.1439793. [DOI] [PubMed] [Google Scholar]
  7. Stepanov V. M., Chestukhina G. G., Rudenskaya G. N., Epremyan A. S., Osterman A. L., Khodova O. M., Belyanova L. P. A new subfamily of microbial serine proteinase? Structural similarities of Bacillus thuringiensis and Thermoactinomyces vulgaris extracellular serine proteinases. Biochem Biophys Res Commun. 1981 Jun;100(4):1680–1687. doi: 10.1016/0006-291x(81)90712-9. [DOI] [PubMed] [Google Scholar]

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