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. 1973 Mar 17;1(5854):645–648. doi: 10.1136/bmj.1.5854.645

Radioimmunoassay of Triiodothyronine in Unextracted Human Serum

R-D Hesch, David Evered
PMCID: PMC1588630  PMID: 4735001

Abstract

Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0·76-1·67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There is a good correlation between serum T-3 and serum thyroxine (T-4) concentrations, and estimation of T-3 seems likely to prove a practical and reliable test of thyroid function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown B. L., Ekins R. P., Ellis S. M., Reith W. S. Specific antibodies to triiodothyronine hormone. Nature. 1970 Apr 25;226(5243):359–359. doi: 10.1038/226359a0. [DOI] [PubMed] [Google Scholar]
  2. Chopra I. J., Solomon D. H., Beall G. N. Radioimmunoassay for measurement of triiodothyronine in human serum. J Clin Invest. 1971 Oct;50(10):2033–2041. doi: 10.1172/JCI106696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Evered D., Hall R. Hypothyroidism. Br Med J. 1972 Jan 29;1(5795):290–293. doi: 10.1136/bmj.1.5795.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GROSS J., PITT-RIVERS R. The identification of 3:5:3'-L-triiodothyronine in human plasma. Lancet. 1952 Mar 1;1(6705):439–441. doi: 10.1016/s0140-6736(52)91952-1. [DOI] [PubMed] [Google Scholar]
  5. Hesch R. D., Hüfner M. Highly specific antibodies to triiodothyronine. Acta Biol Med Ger. 1972;28(5):861–864. [PubMed] [Google Scholar]
  6. Larsen P. R. Direct immunoassay of triiodothyronine in human serum. J Clin Invest. 1972 Aug;51(8):1939–1949. doi: 10.1172/JCI107000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Larsen P. R. Inhibition of triiodothyronine (T3) binding to thyroxine-binding globulin by sodium salicylate and its application to immunoassay of T3 in human serum. Metabolism. 1971 Oct;20(10):976–980. doi: 10.1016/0026-0495(71)90018-7. [DOI] [PubMed] [Google Scholar]
  8. Larsen P. R. Salicylate-induced increases in free triiodothyronine in human serum. Evidence of inhibition of triiodothyronine binding to thyroxine-binding globulin and thyroxine-binding prealbumin. J Clin Invest. 1972 May;51(5):1125–1134. doi: 10.1172/JCI106905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Larsen P. R. Technical aspects of the estimation of triiodothyronine in human serum: evidence of conversion of thyroxine to triiodothyronine during assay. Metabolism. 1971 Jun;20(6):609–624. doi: 10.1016/0026-0495(71)90009-6. [DOI] [PubMed] [Google Scholar]
  10. Mitsuma T., Nihei N., Gershengorn M. C., Hollander C. S. Serum triiodothyronine: measurements in human serum by radioimmunoassay with corroboration by gas-liquid chromatography. J Clin Invest. 1971 Dec;50(12):2679–2688. doi: 10.1172/JCI106769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ormston B. J., Cryer R. J., Garry R., Besser G. M., Hall R. Thyrotrophin-releasing hormone as a thyroid-function test. Lancet. 1971 Jul 3;2(7714):10–14. doi: 10.1016/s0140-6736(71)90005-5. [DOI] [PubMed] [Google Scholar]
  12. Sterling K., Bellabarba D., Newman E. S., Brenner M. A. Determination of triiodothyronine concentration in human serum. J Clin Invest. 1969 Jun;48(6):1150–1158. doi: 10.1172/JCI106072. [DOI] [PMC free article] [PubMed] [Google Scholar]

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