Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1993 Oct;103(2):385–390. doi: 10.1104/pp.103.2.385

Controlled Proteolysis Activates the Plasma Membrane Ca2+ Pump of Higher Plants (A Comparison with the Effect of Calmodulin in Plasma Membrane from Radish Seedlings).

F Rasi-Caldogno 1, A Carnelli 1, M I De Michelis 1
PMCID: PMC158994  PMID: 12231945

Abstract

The effects of calmodulin and of controlled trypsin treatments on the activity of the Ca2+ pump were investigated in plasma membrane purified from radish (Raphanus sativus L.) seedlings. Treatment of the plasma membrane with ethylenediaminetetra-acetate (EDTA), which removed about two-thirds of the plasma membrane-associated calmodulin, markedly increased the stimulation of the Ca2+ pump by calmodulin. In EDTA-treated plasma membrane, stimulation by calmodulin of the Ca2+ pump activity was maximal at low free Ca2+ (2-5 [mu]M) and decreased with the increase of free Ca2+ concentration. The Ca2+ pump activity was stimulated also by a controlled treatment of the plasma membrane with trypsin: the effect of trypsin treatment depended on the concentration of both trypsin and plasma membrane proteins and on the duration of incubation. Stimulation of the Ca2+ pump activity by trypsin treatment of the plasma membrane was similar to that induced by calmodulin both in extent and in dependence on the free Ca2+ concentration in the assay medium. Moreover, the Ca2+ pump of trypsin-treated plasma membrane was insensitive to further stimulation by calmodulin, suggesting that limited proteolysis preferentially cleaves a regulatory domain of the enzyme that is involved in its activation by calmodulin.

Full Text

The Full Text of this article is available as a PDF (581.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askerlund P., Evans D. E. Reconstitution and Characterization of a Calmodulin-Stimulated Ca-Pumping ATPase Purified from Brassica oleracea L. Plant Physiol. 1992 Dec;100(4):1670–1681. doi: 10.1104/pp.100.4.1670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carafoli E. The Ca2+ pump of the plasma membrane. J Biol Chem. 1992 Feb 5;267(4):2115–2118. [PubMed] [Google Scholar]
  3. Carnelli A., De Michelis M. I., Rasi-Caldogno F. Plasma Membrane Ca-ATPase of Radish Seedlings : I. Biochemical Characterization Using ITP as a Substrate. Plant Physiol. 1992 Mar;98(3):1196–1201. doi: 10.1104/pp.98.3.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coelho-Sampaio T., Ferreira S. T., Benaim G., Vieyra A. Dissociation of purified erythrocyte Ca(2+)-ATPase by hydrostatic pressure. J Biol Chem. 1991 Nov 25;266(33):22266–22272. [PubMed] [Google Scholar]
  5. Collinge M., Trewavas A. J. The location of calmodulin in the pea plasma membrane. J Biol Chem. 1989 May 25;264(15):8865–8872. [PubMed] [Google Scholar]
  6. De Michelis M. I., Spanswick R. M. H-pumping driven by the vanadate-sensitive ATPase in membrane vesicles from corn roots. Plant Physiol. 1986 Jun;81(2):542–547. doi: 10.1104/pp.81.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hsieh W. L., Pierce W. S., Sze H. Calcium-pumping ATPases in vesicles from carrot cells : stimulation by calmodulin or phosphatidylserine, and formation of a 120 kilodalton phosphoenzyme. Plant Physiol. 1991 Dec;97(4):1535–1544. doi: 10.1104/pp.97.4.1535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pershadsingh H. A., McDonald J. M. A high affinity calcium-stimulated magnesium-dependent adenosine triphosphatase in rat adipocyte plasma membranes. J Biol Chem. 1980 May 10;255(9):4087–4093. [PubMed] [Google Scholar]
  9. Rasi-Caldogno F., Carnelli A., De Michelis M. I. Plasma Membrane Ca-ATPase of Radish Seedlings : II. Regulation by Calmodulin. Plant Physiol. 1992 Mar;98(3):1202–1206. doi: 10.1104/pp.98.3.1202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rasi-Caldogno F., Pugliarello M. C., De Michelis M. I. The Ca-Transport ATPase of Plant Plasma Membrane Catalyzes a nH/Ca Exchange. Plant Physiol. 1987 Apr;83(4):994–1000. doi: 10.1104/pp.83.4.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rasi-Caldogno F., Pugliarello M. C., Olivari C., De Michelis M. I. Identification and Characterization of the Ca-ATPase which Drives Active Transport of Ca at the Plasma Membrane of Radish Seedlings. Plant Physiol. 1989 Aug;90(4):1429–1434. doi: 10.1104/pp.90.4.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Williams L. E., Schueler S. B., Briskin D. P. Further Characterization of the Red Beet Plasma Membrane Ca-ATPase Using GTP as an Alternative Substrate. Plant Physiol. 1990 Mar;92(3):747–754. doi: 10.1104/pp.92.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES