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. 1993 Oct;103(2):435–440. doi: 10.1104/pp.103.2.435

Comparative Studies on Ferredoxin-NADP+ Oxidoreductase Isoenzymes Derived from Different Organs by Antibodies Specific for the Radish Root- and Leaf-Enzymes.

S Morigasaki 1, T Jin 1, K Wada 1
PMCID: PMC159001  PMID: 12231952

Abstract

Determination of the prosthetic group and titration of sulfhydryl group of ferredoxin-NADP+ oxidoreductase (FNR) from roots of radish (Raphanus sativus var acanthiformis cv Miyashige) confirmed its similarity to leaf-FNR. Antisera directed against radish root-FNR and leaf-FNR distinguished the enzyme forms from roots and leaves of radish as well as other flowering plants. The FNR isoenzymes showed organ-specific distributions. In horsetail (Equisetum arvense L.) and cultured liverwort cells (Marchantia polymorpha), at least two FNR isoenzymes were distinguished by the antisera. FNR from Chlorella vulgaris reacted only with the anti-root-FNR antiserum. FNR from a cyanobacterium, Spirulina spp., failed to react with either antiserum.

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Selected References

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  1. Bookjans G., San Pietro A., Böger P. Resolution and reconstitution of spinach ferredoxin-NADP+ reductase. Biochem Biophys Res Commun. 1978 Feb 28;80(4):759–765. doi: 10.1016/0006-291x(78)91310-4. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  4. CRESTFIELD A. M., MOORE S., STEIN W. H. The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem. 1963 Feb;238:622–627. [PubMed] [Google Scholar]
  5. Fillat M. F., Bakker H. A., Weisbeek P. J. Sequence of the ferredoxin-NADP(+)-reductase gene from Anabaena PCC 7119. Nucleic Acids Res. 1990 Dec 11;18(23):7161–7161. doi: 10.1093/nar/18.23.7161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green L. S., Yee B. C., Buchanan B. B., Kamide K., Sanada Y., Wada K. Ferredoxin and ferredoxin-NADP reductase from photosynthetic and nonphotosynthetic tissues of tomato. Plant Physiol. 1991;96:1207–1213. doi: 10.1104/pp.96.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hase T., Kimata Y., Yonekura K., Matsumura T., Sakakibara H. Molecular cloning and differential expression of the maize ferredoxin gene family. Plant Physiol. 1991 May;96(1):77–83. doi: 10.1104/pp.96.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hasumi H., Nagata E., Nakamura S. Molecular heterogeneity of ferredoxin-NADP+ reductase from spinach leaves. Biochem Biophys Res Commun. 1983 Jan 14;110(1):280–286. doi: 10.1016/0006-291x(83)91292-5. [DOI] [PubMed] [Google Scholar]
  9. Jansen T., Reiländer H., Steppuhn J., Herrmann R. G. Analysis of cDNA clones encoding the entire precursor-polypeptide for ferredoxin:NADP+ oxidoreductase from spinach. Curr Genet. 1988 Jun;13(6):517–522. doi: 10.1007/BF02427758. [DOI] [PubMed] [Google Scholar]
  10. Karplus P. A., Walsh K. A., Herriott J. R. Amino acid sequence of spinach ferredoxin:NADP+ oxidoreductase. Biochemistry. 1984 Dec 18;23(26):6576–6583. doi: 10.1021/bi00321a046. [DOI] [PubMed] [Google Scholar]
  11. Kimata Y., Hase T. Localization of ferredoxin isoproteins in mesophyll and bundle sheath cells in maize leaf. Plant Physiol. 1989 Apr;89(4):1193–1197. doi: 10.1104/pp.89.4.1193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Matsubara H., Wada K. Soluble cytochromes and ferredoxins. Methods Enzymol. 1988;167:387–410. doi: 10.1016/0076-6879(88)67044-3. [DOI] [PubMed] [Google Scholar]
  14. Morigasaki S., Takata K., Suzuki T., Wada K. Purification and Characterization of a Ferredoxin-NADP Oxidoreductase-Like Enzyme from Radish Root Tissues. Plant Physiol. 1990 Jul;93(3):896–901. doi: 10.1104/pp.93.3.896. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Solomonson L. P., Lorimer G. H., Hall R. L., Borchers R., Bailey J. L. Reduced nicotinamide adenine dinucleotide-nitrate reductase of Chlorella vulgaris. Purification, prosthetic groups, and molecular properties. J Biol Chem. 1975 Jun 10;250(11):4120–4127. [PubMed] [Google Scholar]
  16. Suzuki A., Oaks A., Jacquot J. P., Vidal J., Gadal P. An electron transport system in maize roots for reactions of glutamate synthase and nitrite reductase : physiological and immunochemical properties of the electron carrier and pyridine nucleotide reductase. Plant Physiol. 1985 Jun;78(2):374–378. doi: 10.1104/pp.78.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Suzuki A., Vidal J., Gadal P. Glutamate synthase isoforms in rice: immunological studies of enzymes in green leaf, etiolated leaf, and root tissues. Plant Physiol. 1982 Sep;70(3):827–832. doi: 10.1104/pp.70.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wada K., Onda M., Matsubara H. Amino acid sequences of ferredoxin isoproteins from radish roots. J Biochem. 1989 Apr;105(4):619–625. doi: 10.1093/oxfordjournals.jbchem.a122714. [DOI] [PubMed] [Google Scholar]
  19. Wada K., Tamura T., Matsubara H., Kodo K. Spirulina ferredoxin-NADP+ reductase. Further characterization with an improved preparation. J Biochem. 1983 Aug;94(2):387–393. doi: 10.1093/oxfordjournals.jbchem.a134367. [DOI] [PubMed] [Google Scholar]
  20. Yao Y., Tamura T., Wada K., Matsubara H., Kodo K. Spirulina ferredoxin-NADP+ reductase. The complete amino acid sequence. J Biochem. 1984 May;95(5):1513–1516. doi: 10.1093/oxfordjournals.jbchem.a134759. [DOI] [PubMed] [Google Scholar]
  21. Yao Y., Wada K., Takahashi Y., Katoh S., Matsubara H. The sulfhydryl groups of ferredoxin-NADP+ oxidoreductases: is a disulfide bond really present? J Biochem. 1985 Oct;98(4):1079–1082. doi: 10.1093/oxfordjournals.jbchem.a135355. [DOI] [PubMed] [Google Scholar]

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