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. 1994 Feb;104(2):479–496. doi: 10.1104/pp.104.2.479

A Contrast of the Plasma Membrane Lipid Composition of Oat and Rye Leaves in Relation to Freezing Tolerance.

M Uemura 1, P L Steponkus 1
PMCID: PMC159222  PMID: 12232097

Abstract

The lipid composition of the plasma membrane isolated from leaves of spring oat (Avena sativa L. cv Ogle) was vastly different from that of winter rye (Secale cereale L. cv Puma). The plasma membrane of spring oat contained large proportions of phospholipids (28.8 mol% of the total lipids), cerebrosides (27.2 mol%), and acylated sterylglucosides (27.3 mol%) with lesser proportions of free sterols (8.4 mol%) and sterylglucosides (5.6 mol%). In contrast, the plasma membrane of winter rye contained a greater proportion of phospholipids (36.6 mol%), and there was a lower proportion of cerebrosides (16.4 mol%); free sterols (38.1 mol%) were the predominant sterols, with lesser proportions of sterylglucosides (5.6 mol%) and acylated sterylglucosides (2.9 mol%). Although the relative proportions of individual phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine, and the molecular species of these two phospholipids were similar in oat and rye, the relative proportions of di-unsaturated species of these two phospholipids were substantially lower in oat than in rye. The relative proportions of sterol species in oat were different from those in rye; the molecular species of cerebrosides were similar in oat and rye, with only slight differences in the proportions of the individual species. After 4 weeks of cold acclimation, the proportion of phospholipids increased significantly in both oat (from 28.8 to 36.8 mol%) and rye (from 36.6 to 43.3 mol%) as a result of increases in the proportions of phosphatidylcholine and phosphatidylethanolamine. For both oat and rye, the relative proportions of di-unsaturated species increased after cold acclimation, but the increase was greater in rye than in oat. In both oat and rye, this increase occurred largely during the first week of cold acclimation. During the 4 weeks of cold acclimation, there was a progressive decrease in the proportion of cerebrosides in the plasma membrane of rye (from 16.4 to 10.5 mol%), but there was only a small decrease in oat (from 27.2 to 24.2 mol%). In both oat and rye, there were only small changes in the proportions of free sterols and sterol derivatives during cold acclimation. Consequently, the proportions of both acylated sterylglucosides and cerebrosides remained substantially higher in oat than in rye after cold acclimation. The relationship between these differences in the plasma membrane lipid composition of oat and rye and their freezing tolerance is presented.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Boggs J. M. Lipid intermolecular hydrogen bonding: influence on structural organization and membrane function. Biochim Biophys Acta. 1987 Oct 5;906(3):353–404. doi: 10.1016/0304-4157(87)90017-7. [DOI] [PubMed] [Google Scholar]
  3. Bryant G., Wolfe J. Can hydration forces induce lateral phase separations in lamellar phases? Eur Biophys J. 1989;16(6):369–374. doi: 10.1007/BF00257886. [DOI] [PubMed] [Google Scholar]
  4. Cahoon E. B., Lynch D. V. Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane. Plant Physiol. 1991 Jan;95(1):58–68. doi: 10.1104/pp.95.1.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dowgert M. F., Steponkus P. L. Behavior of the Plasma Membrane of Isolated Protoplasts during a Freeze-Thaw Cycle. Plant Physiol. 1984 Aug;75(4):1139–1151. doi: 10.1104/pp.75.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grenier G., Willemot C. Lipid changes in roots of frost hardy and less hardy alfalfa varieties under hardening conditions. Cryobiology. 1974 Aug;11(4):324–331. doi: 10.1016/0011-2240(74)90009-1. [DOI] [PubMed] [Google Scholar]
  7. Lynch D. V., Caffrey M., Hogan J. L., Steponkus P. L. Calorimetric and x-ray diffraction studies of rye glucocerebroside mesomorphism. Biophys J. 1992 May;61(5):1289–1300. doi: 10.1016/S0006-3495(92)81937-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lynch D. V., Thompson G. A. Microsomal Phospholipid Molecular Species Alterations during Low Temperature Acclimation in Dunaliella. Plant Physiol. 1984 Feb;74(2):193–197. doi: 10.1104/pp.74.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Myher J. J., Kuksis A. Resolution of diacylglycerol moieties of natural glycerophospholipids by gas-liquid chromatography on polar capillary columns. Can J Biochem. 1982 Jun;60(6):638–650. doi: 10.1139/o82-079. [DOI] [PubMed] [Google Scholar]
  10. Raison J. K., Lyons J. M., Mehlhorn R. J., Keith A. D. Temperature-induced phase changes in mitochondrial membranes detected by spin labeling. J Biol Chem. 1971 Jun 25;246(12):4036–4040. [PubMed] [Google Scholar]
  11. Siegel D. P. Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal amphiphile phases. III. Isotropic and inverted cubic state formation via intermediates in transitions between L alpha and HII phases. Chem Phys Lipids. 1986 Dec 31;42(4):279–301. doi: 10.1016/0009-3084(86)90087-3. [DOI] [PubMed] [Google Scholar]
  12. Siegel D. P. Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal lipid phases. II. Implications for membrane-membrane interactions and membrane fusion. Biophys J. 1986 Jun;49(6):1171–1183. doi: 10.1016/S0006-3495(86)83745-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Slater J. L., Huang C. H. Interdigitated bilayer membranes. Prog Lipid Res. 1988;27(4):325–359. doi: 10.1016/0163-7827(88)90010-0. [DOI] [PubMed] [Google Scholar]
  14. Steponkus P. L., Uemura M., Balsamo R. A., Arvinte T., Lynch D. V. Transformation of the cryobehavior of rye protoplasts by modification of the plasma membrane lipid composition. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9026–9030. doi: 10.1073/pnas.85.23.9026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tate M. W., Eikenberry E. F., Turner D. C., Shyamsunder E., Gruner S. M. Nonbilayer phases of membrane lipids. Chem Phys Lipids. 1991 Mar;57(2-3):147–164. doi: 10.1016/0009-3084(91)90073-k. [DOI] [PubMed] [Google Scholar]
  16. Uemura M., Steponkus P. L. Effect of cold acclimation on the incidence of two forms of freezing injury in protoplasts isolated from rye leaves. Plant Physiol. 1989 Nov;91(3):1131–1137. doi: 10.1104/pp.91.3.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Uemura M., Yoshida S. Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma). Plant Physiol. 1984 Jul;75(3):818–826. doi: 10.1104/pp.75.3.818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Uemura M., Yoshida S. Isolation and Identification of Plasma Membrane from Light-Grown Winter Rye Seedlings (Secale cereale L. cv Puma). Plant Physiol. 1983 Nov;73(3):586–597. doi: 10.1104/pp.73.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Webb M. S., Hui S. W., Steponkus P. L. Dehydration-induced lamellar-to-hexagonal-II phase transitions in DOPE/DOPC mixtures. Biochim Biophys Acta. 1993 Jan 18;1145(1):93–104. doi: 10.1016/0005-2736(93)90385-d. [DOI] [PubMed] [Google Scholar]
  20. Webb M. S., Uemura M., Steponkus P. L. A Comparison of Freezing Injury in Oat and Rye: Two Cereals at the Extremes of Freezing Tolerance. Plant Physiol. 1994 Feb;104(2):467–478. doi: 10.1104/pp.104.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Willemot C., Hope H. J., Williams R. J., Michaud R. Changes in fatty acid composition of winter wheat during frost hardening. Cryobiology. 1977 Feb;14(1):87–93. doi: 10.1016/0011-2240(77)90126-2. [DOI] [PubMed] [Google Scholar]
  22. Yoshida S., Uemura M. Lipid Composition of Plasma Membranes and Tonoplasts Isolated from Etiolated Seedlings of Mung Bean (Vigna radiata L.). Plant Physiol. 1986 Nov;82(3):807–812. doi: 10.1104/pp.82.3.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yoshida S., Uemura M. Protein and Lipid Compositions of Isolated Plasma Membranes from Orchard Grass (Dactylis glomerata L.) and Changes during Cold Acclimation. Plant Physiol. 1984 May;75(1):31–37. doi: 10.1104/pp.75.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zlatkis A., Zak B. Study of a new cholesterol reagent. Anal Biochem. 1969 Apr 11;29(1):143–148. doi: 10.1016/0003-2697(69)90017-7. [DOI] [PubMed] [Google Scholar]
  25. de la Roche I. A., Pomeroy M. K., Andrews C. J. Changes in fatty acid composition in wheat cultivars of contrasting hardiness. Cryobiology. 1975 Oct;12(5):506–512. doi: 10.1016/0011-2240(75)90032-2. [DOI] [PubMed] [Google Scholar]

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