Abstract
The time course of suberization in wound periderm from potato (Solanum tuberosum L.) has been monitored by histochemical and high-resolution solid-state nuclear magnetic resonance (NMR) methods. Light microscopy conducted after selective staining of the lipid and double-bonded constituents shows that suberin is deposited at the outermost intact cell-wall surface during the first 7 d of wound healing; suberization forms a barrier to tissue infiltration at later times. Cross polarization-magic angle spinning 13C NMR spectra demonstrate the deposition of a polyester containing all major suberin functional groups after just 4 d of wound healing. Initially the suberin includes a large proportion of aromatic groups and fairly short aliphatic chains, but the spectral data demonstrate the growing dominance of long-chain species during the period 7 to 14 d after wounding. The results of preliminary 13C-labeling experiments with sodium [2-13C]acetate and DL-[1-13C]phenylalanine provide an excellent prospectus for future NMR-based studies of suberin biosynthesis.
Full Text
The Full Text of this article is available as a PDF (1.8 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bernards M. A., Lewis N. G. Alkyl ferulates in wound healing potato tubers. Phytochemistry. 1992 Oct;31(10):3409–3412. doi: 10.1016/0031-9422(92)83695-u. [DOI] [PubMed] [Google Scholar]
- Bretthorst G. L., Kotyk J. J., Ackerman J. J. 31P NMR Bayesian spectral analysis of rat brain in vivo. Magn Reson Med. 1989 Feb;9(2):282–287. doi: 10.1002/mrm.1910090214. [DOI] [PubMed] [Google Scholar]
- Dean B. B., Kolattukudy P. E. Biochemistry of Suberization: Incorporation of [1-C]Oleic Acid and [1-C]Acetate into the Aliphatic Components of Suberin in Potato Tuber Disks (Solanum tuberosum). Plant Physiol. 1977 Jan;59(1):48–54. doi: 10.1104/pp.59.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean B. B., Kolattukudy P. E., Davis R. W. Chemical Composition and Ultrastructure of Suberin from Hollow Heart Tissue of Potato Tubers (Solanum tuberosum). Plant Physiol. 1977 May;59(5):1008–1010. doi: 10.1104/pp.59.5.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fukamizo T., Kramer K. J., Mueller D. D., Schaefer J., Garbow J., Jacob G. S. Analysis of chitin structure by nuclear magnetic resonance spectroscopy and chitinolytic enzyme digestion. Arch Biochem Biophys. 1986 Aug 15;249(1):15–26. doi: 10.1016/0003-9861(86)90555-2. [DOI] [PubMed] [Google Scholar]
- Kolattukudy P. E. Biopolyester membranes of plants: cutin and suberin. Science. 1980 May 30;208(4447):990–1000. doi: 10.1126/science.208.4447.990. [DOI] [PubMed] [Google Scholar]
- Kolattukudy P. E., Dean B. B. Structure, gas chromatographic measurement, and function of suberin synthesized by potato tuber tissue slices. Plant Physiol. 1974 Jul;54(1):116–121. doi: 10.1104/pp.54.1.116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis N. G., Yamamoto E., Wooten J. B., Just G., Ohashi H., Towers G. H. Monitoring biosynthesis of wheat cell-wall phenylpropanoids in situ. Science. 1987 Sep 11;237(4820):1344–1346. doi: 10.1126/science.237.4820.1344. [DOI] [PubMed] [Google Scholar]