Abstract
Cellulase activity was measured in soybean (Glycine max) leaf abscission zones, flower abscission zones, pod abscission zones, apical buds, and adventitious rooting hypocotyls. Immunoprecipitation data showed that a cellulase immunologically similar to the bean abscission cellulase (isoelectric point 9.5) is present in soybean leaf, flower, and pod abscission zones, but is not present in soybean apical buds or rooting hypocotyls. cDNA and genomic clones for two different soybean genes were identified and show sequence similarity with the bean abscission cellulase clone pBAC10. The cDNA clone pSAC1, isolated from a soybean abscission cDNA library, hybridized to transcripts in soybean leaf, flower, and pod abscission zones. Although ethylene has been shown to play a role in the increase in cellulase activity associated with both abscission and adventitious root initiation, no signal was seen for hybridization of the soybean abscission cellulase clone, pSAC1, to RNA from soybean adventitious rooting hypocotyls. In addition, no soybean abscission cellulase transcripts were detected in apical buds. Transcripts for a second soybean cellulase gene (SC2) were not detected in any of the tissues surveyed.
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- Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cass L. G., Kirven K. A., Christoffersen R. E. Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening. Mol Gen Genet. 1990 Aug;223(1):76–86. doi: 10.1007/BF00315799. [DOI] [PubMed] [Google Scholar]
- Del Campillo E., Lewis L. N. Occurrence of 9.5 cellulase and other hydrolases in flower reproductive organs undergoing major cell wall disruption. Plant Physiol. 1992 Jul;99(3):1015–1020. doi: 10.1104/pp.99.3.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Del Campillo E., Reid P. D., Sexton R., Lewis L. N. Occurrence and Localization of 9.5 Cellulase in Abscising and Nonabscising Tissues. Plant Cell. 1990 Mar;2(3):245–254. doi: 10.1105/tpc.2.3.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson A. O., Larkins B. A. Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves. Plant Physiol. 1976 Jan;57(1):5–10. doi: 10.1104/pp.57.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Linkins A. E., Lewis L. N., Palmer R. L. Hormonally Induced Changes in the Stem and Petiole Anatomy and Cellulase Enzyme Patterns in Phaseolus vulgaris L. Plant Physiol. 1973 Dec;52(6):554–560. doi: 10.1104/pp.52.6.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tucker M. L., Sexton R., Del Campillo E., Lewis L. N. Bean abscission cellulase : characterization of a cDNA clone and regulation of gene expression by ethylene and auxin. Plant Physiol. 1988 Dec;88(4):1257–1262. doi: 10.1104/pp.88.4.1257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yu Y. B., Yang S. F. Auxin-induced Ethylene Production and Its Inhibition by Aminoethyoxyvinylglycine and Cobalt Ion. Plant Physiol. 1979 Dec;64(6):1074–1077. doi: 10.1104/pp.64.6.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]