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. 1994 May;105(1):173–179. doi: 10.1104/pp.105.1.173

Responses of Ribulose-1,5-Bisphosphate Carboxylase, Cytochrome f, and Sucrose Synthesis Enzymes in Rice Leaves to Leaf Nitrogen and Their Relationships to Photosynthesis.

A Makino 1, H Nakano 1, T Mae 1
PMCID: PMC159343  PMID: 12232197

Abstract

The photosynthetic gas-exchange rates and various biochemical components of photosynthesis, including ribulose-1,5-bisphosphate carboxylase (Rubisco) content, cytochrome (Cyt) f content, and the activities of two sucrose synthesis enzymes, were examined in young, fully expanded leaves of rice (Oryza sativa L.) grown hydroponically in different nitrogen concentrations. The light-saturated rate of photosynthesis at an intercellular CO2 pressure of 20 Pa (CO2-limited photosynthesis) was linearly dependent on leaf nitrogen content, but curvilinearly correlated with Rubisco content. This difference was due to a greater than proportional increase in Rubisco content relative to leaf nitrogen content and the presence of a CO2 transfer resistance between the intercellular air spaces and the carboxylation sites. CO2-limited photosynthesis was proportional to Cyt f content, one of the key components of electron transport, but was not proportional to the activities of cytosolic fructose-1,6-bisphosphatase and sucrose phosphate synthase, the two regulatory enzymes of sucrose synthesis. Light-saturated photosynthesis above an intercellular CO2 pressure of 60 Pa (CO2-saturated photosynthesis) was curvilinearly dependent on leaf nitrogen content. This CO2-saturated photosynthesis was proportional to Cyt f content in the low- and normal-nitrogen leaves, and correlated better with the activities of cytosolic fructose-1,6-bisphosphatase and sucrose phosphate synthase in the high-nitrogen leaves. The increase in the activities of these two enzymes with increasing leaf nitrogen was not as great as the increase in Cyt f content. Thus, as leaf nitrogen increased, the limitation caused by the activities of sucrose synthesis enzymes came into play, which resulted in the curvilinear relationship. However, this limitation by sucrose synthesis enzymes did not affect photosynthesis under normal ambient air.

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Selected References

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  1. Evans J. R. Nitrogen and Photosynthesis in the Flag Leaf of Wheat (Triticum aestivum L.). Plant Physiol. 1983 Jun;72(2):297–302. doi: 10.1104/pp.72.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harley P. C., Loreto F., Di Marco G., Sharkey T. D. Theoretical Considerations when Estimating the Mesophyll Conductance to CO(2) Flux by Analysis of the Response of Photosynthesis to CO(2). Plant Physiol. 1992 Apr;98(4):1429–1436. doi: 10.1104/pp.98.4.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hidema J., Makino A., Mae T., Ojima K. Photosynthetic Characteristics of Rice Leaves Aged under Different Irradiances from Full Expansion through Senescence. Plant Physiol. 1991 Dec;97(4):1287–1293. doi: 10.1104/pp.97.4.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holloway P. J., Maclean D. J., Scott K. J. Rate-Limiting Steps of Electron Transport in Chloroplasts during Ontogeny and Senescence of Barley. Plant Physiol. 1983 Jul;72(3):795–801. doi: 10.1104/pp.72.3.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huber S. C. Role of sucrose-phosphate synthase in partitioning of carbon in leaves. Plant Physiol. 1983 Apr;71(4):818–821. doi: 10.1104/pp.71.4.818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loreto F., Harley P. C., Di Marco G., Sharkey T. D. Estimation of Mesophyll Conductance to CO(2) Flux by Three Different Methods. Plant Physiol. 1992 Apr;98(4):1437–1443. doi: 10.1104/pp.98.4.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Makino A., Osmond B. Effects of Nitrogen Nutrition on Nitrogen Partitioning between Chloroplasts and Mitochondria in Pea and Wheat. Plant Physiol. 1991 Jun;96(2):355–362. doi: 10.1104/pp.96.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Makino A., Sakashita H., Hidema J., Mae T., Ojima K., Osmond B. Distinctive Responses of Ribulose-1,5-Bisphosphate Carboxylase and Carbonic Anhydrase in Wheat Leaves to Nitrogen Nutrition and their Possible Relationships to CO(2)-Transfer Resistance. Plant Physiol. 1992 Dec;100(4):1737–1743. doi: 10.1104/pp.100.4.1737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sage R. F., Sharkey T. D., Seemann J. R. Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Light Intensity and CO(2) in the C(3) Annuals Chenopodium album L. and Phaseolus vulgaris L. Plant Physiol. 1990 Dec;94(4):1735–1742. doi: 10.1104/pp.94.4.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sage R. F., Sharkey T. D. The Effect of Temperature on the Occurrence of O(2) and CO(2) Insensitive Photosynthesis in Field Grown Plants. Plant Physiol. 1987 Jul;84(3):658–664. doi: 10.1104/pp.84.3.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sharkey T. D., Savitch L. V., Vanderveer P. J., Micallef B. J. Carbon Partitioning in a Flaveria linearis Mutant with Reduced Cytosolic Fructose Bisphosphatase. Plant Physiol. 1992 Sep;100(1):210–215. doi: 10.1104/pp.100.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]

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