Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1994 Jun;105(2):519–527. doi: 10.1104/pp.105.2.519

Photopolarization of the Fucus sp. Zygote by Blue Light Involves a Plasma Membrane Redox Chain.

F Berger 1, C Brownlee 1
PMCID: PMC159389  PMID: 12232219

Abstract

Zygotes of fucoid algae are photopolarized by unidirectional blue light (BL). Polar axes are formed, fixed, and expressed by germination of a rhizoid. Hexacyanoferrate(III) ions (HCF) specifically inhibit transduction of the BL signal. HCF reduction by Fucus sp. zygotes occurs on the outer surface of the plasma membrane at higher rates in BL than in dark. These observations suggest that BL signal transduction involves a redox chain in the plasma membrane. Low doses of HCF (<50 pmol cell-1) inhibit photopolarization but not germination, hence uncoupling both processes. Exposure during the photosensitive period to higher doses of HCF together with BL significantly inhibits germination. Further results suggest that BL transduction is dependent on photosynthetic products that could also interact with redox processes.

Full Text

The Full Text of this article is available as a PDF (1.6 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amodeo G., Srivastava A., Zeiger E. Vanadate inhibits blue light-stimulated swelling of vicia guard cell protoplasts. Plant Physiol. 1992 Nov;100(3):1567–1570. doi: 10.1104/pp.100.3.1567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berger F., Brownlee C. Ratio confocal imaging of free cytoplasmic calcium gradients in polarising and polarised Fucus zygotes. Zygote. 1993 Feb;1(1):9–15. doi: 10.1017/s0967199400001246. [DOI] [PubMed] [Google Scholar]
  3. Brawley S. H., Wetherbee R., Quatrano R. S. Fine-structural studies of the gametes and embryo of Fucus vesiculosus L. (Phaeophyta). II. The cytoplasm of the egg and young zygote. J Cell Sci. 1976 Mar;20(2):255–271. doi: 10.1242/jcs.20.2.255. [DOI] [PubMed] [Google Scholar]
  4. Chory J. Out of darkness: mutants reveal pathways controlling light-regulated development in plants. Trends Genet. 1993 May;9(5):167–172. doi: 10.1016/0168-9525(93)90163-c. [DOI] [PubMed] [Google Scholar]
  5. Gautier H., Vavasseur A., Lascève G., Boudet A. M. Redox Processes in the Blue Light Response of Guard Cell Protoplasts of Commelina communis L. Plant Physiol. 1992 Jan;98(1):34–38. doi: 10.1104/pp.98.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibbon B. C., Kropf D. L. Intracellular pH and its regulation in Pelvetia zygotes. Dev Biol. 1993 May;157(1):259–268. doi: 10.1006/dbio.1993.1130. [DOI] [PubMed] [Google Scholar]
  7. JAFFE L. F. Tropistic responses of zygotes of the Fucaceae to polarized light. Exp Cell Res. 1958 Oct;15(2):282–299. doi: 10.1016/0014-4827(58)90031-4. [DOI] [PubMed] [Google Scholar]
  8. Kaufman L. S. Transduction of Blue-Light Signals. Plant Physiol. 1993 Jun;102(2):333–337. doi: 10.1104/pp.102.2.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kropf D. L. Establishment and expression of cellular polarity in fucoid zygotes. Microbiol Rev. 1992 Jun;56(2):316–339. doi: 10.1128/mr.56.2.316-339.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kropf D. L., Hopkins R., Quatrano R. S. Protein synthesis and morphogenesis are not tightly linked during embryogenesis in Fucus. Dev Biol. 1989 Aug;134(2):451–461. doi: 10.1016/0012-1606(89)90118-8. [DOI] [PubMed] [Google Scholar]
  11. Marrè M. T., Moroni A., Albergoni F. G., Marrè E. Plasmalemma redox activity and h extrusion: I. Activation of the h-pump by ferricyanide-induced potential depolarization and cytoplasm acidification. Plant Physiol. 1988 May;87(1):25–29. doi: 10.1104/pp.87.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Quatrano R. S. Separation of processes associated with differentiation of two-celled Fucus embryos. Dev Biol. 1973 Jan;30(1):209–213. doi: 10.1016/0012-1606(73)90059-6. [DOI] [PubMed] [Google Scholar]
  13. Vani T., Raghavendra A. S. Tetrazolium Reduction by Guard Cells in Abaxial Epidermis of Vicia faba: Blue Light Stimulation of a Plasmalemma Redox System. Plant Physiol. 1989 May;90(1):59–62. doi: 10.1104/pp.90.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zeiger E., Hepler P. K. Light and stomatal function: blue light stimulates swelling of guard cell protoplasts. Science. 1977 May 20;196(4292):887–889. doi: 10.1126/science.196.4292.887. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES