Abstract
Seedlings of 17 rice (Oryza sativa L.) cultivars were classified on the basis of anthocyanin pigmentation into three groups: an acyanic group with 9 cultivars, a moderately cyanic group with 5 cultivars, and a cyanic group with 3 cultivars. Seedlings of the cyanic group were deep purple in color, possessing copious amounts of anthocyanin in shoots. Sunlight (SL)-mediated anthocyanin and phenylalanine ammonia lyase (PAL) induction in a cyanic cultivar, purple puttu, was compared with an acyanic cultivar, black puttu. A brief exposure of dark-grown purple puttu seedlings to SL induced anthocyanin formation during a subsequent dark period with a peak at 24 h. The magnitude of SL-mediated anthocyanin induction is age dependent, the 4-d-old seedlings being the most responsive to SL. The anthocyanin induction in purple puttu seedlings is mediated exclusively by the ultraviolet-B (UV-B) component of SL. The SL-triggered anthocyanin induction was reduced by about 30% by a terminal far-red light pulse and was restored by a red light pulse, indicating the role of phytochrome in modulation of anthocyanin level. The SL-mediated induction of PAL showed two peaks, one at 4 h and the other at 12 h. Whereas the first PAL peak (4 h) was induced by phytochrome and was seen in both cultivars, the second PAL peak (12 h) was inducible by UV-B only in the cyanic purple puttu cultivar.
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- Dangl J. L., Hauffe K. D., Lipphardt S., Hahlbrock K., Scheel D. Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor. EMBO J. 1987 Sep;6(9):2551–2556. doi: 10.1002/j.1460-2075.1987.tb02543.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kubasek W. L., Shirley B. W., McKillop A., Goodman H. M., Briggs W., Ausubel F. M. Regulation of Flavonoid Biosynthetic Genes in Germinating Arabidopsis Seedlings. Plant Cell. 1992 Oct;4(10):1229–1236. doi: 10.1105/tpc.4.10.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li J., Ou-Lee T. M., Raba R., Amundson R. G., Last R. L. Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation. Plant Cell. 1993 Feb;5(2):171–179. doi: 10.1105/tpc.5.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lloyd A. M., Walbot V., Davis R. W. Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992 Dec 11;258(5089):1773–1775. doi: 10.1126/science.1465611. [DOI] [PubMed] [Google Scholar]
- Minami E., Ozeki Y., Matsuoka M., Koizuka N., Tanaka Y. Structure and some characterization of the gene for phenylalanine ammonia-lyase from rice plants. Eur J Biochem. 1989 Oct 20;185(1):19–25. doi: 10.1111/j.1432-1033.1989.tb15075.x. [DOI] [PubMed] [Google Scholar]
- Schmelzer E., Jahnen W., Hahlbrock K. In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves. Proc Natl Acad Sci U S A. 1988 May;85(9):2989–2993. doi: 10.1073/pnas.85.9.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yatsuhashi H., Hashimoto T., Shimizu S. Ultraviolet action spectrum for anthocyanin formation in broom sorghum first internodes. Plant Physiol. 1982 Sep;70(3):735–741. doi: 10.1104/pp.70.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ziska L. H., Teramura A. H. CO(2) Enhancement of Growth and Photosynthesis in Rice (Oryza sativa) : Modification by Increased Ultraviolet-B Radiation. Plant Physiol. 1992 Jun;99(2):473–481. doi: 10.1104/pp.99.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]