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. 1994 Aug;105(4):1223–1229. doi: 10.1104/pp.105.4.1223

Transcriptional and Posttranscriptional Regulation of Nitrogen-Responding Expression of Phosphoenolpyruvate Carboxylase Gene in Maize.

I Suzuki 1, C Cretin 1, T Omata 1, T Sugiyama 1
PMCID: PMC159452  PMID: 12232278

Abstract

To study the regulation of gene expression for enzymes in the C4 photosynthetic pathway of maize (Zea mays L.) in response to changing N status in developing photosynthetic cells, we have studied in vitro transcription of the phosphoenolpyruvate carboxylase (PEPC) gene in leaf nuclei isolated from plants during recovery from N starvation. The induction was specific for the C4-type PEPC gene (C4Ppc1), and its transcription was N dependent and increased markedly by supply of an N source, but there was a discrepancy between the steady-state levels of mRNA and the stimulation of in vitro transcription. The results suggest that the N-inducible expression of C4Ppc1 is regulated both transcriptionally and posttranscriptionally by N availability. The in vitro transcription rate of C4Ppc1 was greatly stimulated by incubating detached leaves with zeatin alone, whereas the rate remained essentially unchanged by incubating with an exogenous N source alone. The results, taken together, imply that cytokinins up-regulate the transcription of C4Ppc1 in response to N status, whereas glutamine and/or its metabolite(s) up-regulate the level of the transcript. The transcription was totally inhibited by cycloheximide, indicating that the cytokinin-dependent transcription of C4Ppc1 requires the synthesis of protein.

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Selected References

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  1. Caboche M., Rouzé P. Nitrate reductase: a target for molecular and cellular studies in higher plants. Trends Genet. 1990 Jun;6(6):187–192. doi: 10.1016/0168-9525(90)90175-6. [DOI] [PubMed] [Google Scholar]
  2. Crétin C., Santi S., Keryer E., Lepiniec L., Tagu D., Vidal J., Gadal P. The phosphoenolpyruvate carboxylase gene family of Sorghum: promoter structures, amino acid sequences and expression of genes. Gene. 1991 Mar 1;99(1):87–94. doi: 10.1016/0378-1119(91)90037-c. [DOI] [PubMed] [Google Scholar]
  3. Harpster M. H., Taylor W. C. Maize phosphoenolpyruvate carboxylase. Cloning and characterization of mRNAs encoding isozymic forms. J Biol Chem. 1986 May 5;261(13):6132–6136. [PubMed] [Google Scholar]
  4. Hayakawa S., Matsunaga K., Sugiyama T. Light induction of phosphoenolpyruvate carboxylase in etiolated maize leaf tissue. Plant Physiol. 1981 Jan;67(1):133–138. doi: 10.1104/pp.67.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirel B., Bouet C., King B., Layzell D., Jacobs F., Verma D. P. Glutamine synthetase genes are regulated by ammonia provided externally or by symbiotic nitrogen fixation. EMBO J. 1987 May;6(5):1167–1171. doi: 10.1002/j.1460-2075.1987.tb02350.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Izui K., Ishijima S., Yamaguchi Y., Katagiri F., Murata T., Shigesada K., Sugiyama T., Katsuki H. Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C4-pathway from maize. Nucleic Acids Res. 1986 Feb 25;14(4):1615–1628. doi: 10.1093/nar/14.4.1615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lu J. L., Ertl J. R., Chen C. M. Cytokinin enhancement of the light induction of nitrate reductase transcript levels in etiolated barley leaves. Plant Mol Biol. 1990 Apr;14(4):585–594. doi: 10.1007/BF00027504. [DOI] [PubMed] [Google Scholar]
  8. Lu J. L., Ertl J. R., Chen C. M. Transcriptional regulation of nitrate reductase mRNA levels by cytokinin-abscisic Acid interactions in etiolated barley leaves. Plant Physiol. 1992 Apr;98(4):1255–1260. doi: 10.1104/pp.98.4.1255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Luthe D. S., Quatrano R. S. Transcription in Isolated Wheat Nuclei: I. ISOLATION OF NUCLEI AND ELIMINATION OF ENDOGENOUS RIBONUCLEASE ACTIVITY. Plant Physiol. 1980 Feb;65(2):305–308. doi: 10.1104/pp.65.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miao G. H., Hirel B., Marsolier M. C., Ridge R. W., Verma D. P. Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. Plant Cell. 1991 Jan;3(1):11–22. doi: 10.1105/tpc.3.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Staswick P. E. Novel Regulation of Vegetative Storage Protein Genes. Plant Cell. 1990 Jan;2(1):1–6. doi: 10.1105/tpc.2.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sugiharto B., Burnell J. N., Sugiyama T. Cytokinin Is Required to Induce the Nitrogen-Dependent Accumulation of mRNAs for Phosphoenolpyruvate Carboxylase and Carbonic Anhydrase in Detached Maize Leaves. Plant Physiol. 1992 Sep;100(1):153–156. doi: 10.1104/pp.100.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sugiharto B., Sugiyama T. Effects of Nitrate and Ammonium on Gene Expression of Phosphoenolpyruvate Carboxylase and Nitrogen Metabolism in Maize Leaf Tissue during Recovery from Nitrogen Stress. Plant Physiol. 1992 Apr;98(4):1403–1408. doi: 10.1104/pp.98.4.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sugiharto B., Suzuki I., Burnell J. N., Sugiyama T. Glutamine Induces the N-Dependent Accumulation of mRNAs Encoding Phosphoenolpyruvate Carboxylase and Carbonic Anhydrase in Detached Maize Leaf Tissue. Plant Physiol. 1992 Dec;100(4):2066–2070. doi: 10.1104/pp.100.4.2066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ting I. P., Osmond C. B. Multiple forms of plant phosphoenolpyruvate carboxylase associated with different metabolic pathways. Plant Physiol. 1973 Mar;51(3):448–453. doi: 10.1104/pp.51.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]

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