Abstract
Phosphatidylglycerophosphate synthase (sn-glycerol-3-phosphate:CDP-diacylglycerol phosphatidyltransferase) and phosphatidylglycerophosphate phosphatase were characterized in mung bean (Vigna radiata L.) mitochondria. The synthase has a rather broad pH optimum between 7 and 9, whereas the phosphatase has one of about 7. Both enzymic activities are stimulated by Triton X-100 and require divalent cations but differ in their cation specificities. The synthase shows apparent Km values of 9 and 3 [mu]M for sn-glycerol-3-phosphate and CDP-diacylglycerol, respectively. Phosphatidylglycerophosphate, in contrast to lysophosphatidic and phosphatidic acid, is effectively dephosphorylated by the phosphatase, which exhibits an apparent Km value of 12 [mu]M for its substrate. Each enzyme shows higher activities with the dipalmitoyl species of its substrate than with the dioleoyl species. These substrate specificities of both enzymes are predominantly based on differences in apparent Vmax values.
Full Text
The Full Text of this article is available as a PDF (549.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrews J., Mudd J. B. Phosphatidylglycerol synthesis in pea chloroplasts: pathway and localization. Plant Physiol. 1985 Sep;79(1):259–265. doi: 10.1104/pp.79.1.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bligny R., Douce R. A precise localization of cardiolipin in plant cells. Biochim Biophys Acta. 1980 Feb 22;617(2):254–263. doi: 10.1016/0005-2760(80)90168-x. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Chang Y. Y., Kennedy E. P. Phosphatidyl glycerophosphate phosphatase. J Lipid Res. 1967 Sep;8(5):456–462. [PubMed] [Google Scholar]
- Douce R., Mannella C. A., Bonner W. D., Jr Site of the biosynthesis of CDP-diglyceride in plant mitochondria. Biochem Biophys Res Commun. 1972 Dec 18;49(6):1504–1509. doi: 10.1016/0006-291x(72)90510-4. [DOI] [PubMed] [Google Scholar]
- Edman K., Ericson I. Phospholipid and fatty acid composition in mitochondria from spinach (Spinacia oleracea) leaves and petioles. A comparative study. Biochem J. 1987 Apr 15;243(2):575–578. doi: 10.1042/bj2430575. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frentzen M., Neuburger M., Joyard J., Douce R. Intraorganelle localization and substrate specificities of the mitochondrial acyl-CoA: sn-glycerol-3-phosphate O-acyltransferase and acyl-CoA: 1-acyl-sn-glycerol-3-phosphate O-acyltransferase from potato tubers and pea leaves. Eur J Biochem. 1990 Jan 26;187(2):395–402. doi: 10.1111/j.1432-1033.1990.tb15317.x. [DOI] [PubMed] [Google Scholar]
- Icho T. Membrane-bound phosphatases in Escherichia coli: sequence of the pgpA gene. J Bacteriol. 1988 Nov;170(11):5110–5116. doi: 10.1128/jb.170.11.5110-5116.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Icho T., Raetz C. R. Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors. J Bacteriol. 1983 Feb;153(2):722–730. doi: 10.1128/jb.153.2.722-730.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonald P. M., McMurray W. C. Partial purification and properties of mammalian phosphatidylglycerophosphatase. Biochim Biophys Acta. 1980 Oct 6;620(1):80–89. doi: 10.1016/0005-2760(80)90187-3. [DOI] [PubMed] [Google Scholar]
- Marshall M. O., Kates M. Biosynthesis of phosphatidylglycerol by cell-free preparations from spinach leaves. Biochim Biophys Acta. 1972 Apr 18;260(4):558–570. doi: 10.1016/0005-2760(72)90005-7. [DOI] [PubMed] [Google Scholar]
- Moore T. S. Phosphatidylglycerol synthesis in castor bean endosperm: kinetics, requirements, and intracellular localization. Plant Physiol. 1974 Aug;54(2):164–168. doi: 10.1104/pp.54.2.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neuburger M., Journet E. P., Bligny R., Carde J. P., Douce R. Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys. 1982 Aug;217(1):312–323. doi: 10.1016/0003-9861(82)90507-0. [DOI] [PubMed] [Google Scholar]
- Poorthuis BJHM, Hostetler K. Y. Biosynthesis of bis(monoacylglyceryl)phosphate and acylphosphatidylglycerol in rat liver mitochondrial. J Biol Chem. 1975 May 10;250(9):3297–3302. [PubMed] [Google Scholar]
- Robinson N. C. Functional binding of cardiolipin to cytochrome c oxidase. J Bioenerg Biomembr. 1993 Apr;25(2):153–163. doi: 10.1007/BF00762857. [DOI] [PubMed] [Google Scholar]
- Schlame M., Brody S., Hostetler K. Y. Mitochondrial cardiolipin in diverse eukaryotes. Comparison of biosynthetic reactions and molecular acyl species. Eur J Biochem. 1993 Mar 15;212(3):727–735. doi: 10.1111/j.1432-1033.1993.tb17711.x. [DOI] [PubMed] [Google Scholar]
- Stuhne-Sekalec L., Stanacev N. Z. Modification of the biosynthesis and composition of polyglycerophosphatides in outer and inner mitochondrial membranes by cytidine liponucleotides. Membr Biochem. 1989;8(3):165–175. doi: 10.3109/09687688909025829. [DOI] [PubMed] [Google Scholar]
- Tamai K. T., Greenberg M. L. Biochemical characterization and regulation of cardiolipin synthase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1990 Sep 18;1046(2):214–222. doi: 10.1016/0005-2760(90)90192-z. [DOI] [PubMed] [Google Scholar]
- Zwaal R. F., Roelofsen B. Phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus. Methods Enzymol. 1974;32:154–161. doi: 10.1016/0076-6879(74)32019-8. [DOI] [PubMed] [Google Scholar]