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. 1994 Oct;106(2):713–722. doi: 10.1104/pp.106.2.713

Coordinated Transcriptional Regulation of Storage Product Genes in the Maize Endosperm.

M J Giroux 1, C Boyer 1, G Feix 1, L C Hannah 1
PMCID: PMC159579  PMID: 12232363

Abstract

We have demonstrated that expression of genes involved in starch and storage protein synthesis of the maize (Zea mays L.) endosperm are coordinated. Genetic lesions altering synthetic events in one biosynthetic pathway affect expression of genes in both pathways. Initial studies focused on shrunken2 (sh2) and brittle2 (bt2) mutants because these genes encode subunits of the same enzyme, ADP-glucose pyrophosphorylase. Analysis of various sh2- and bt2- mutant alleles showed that the most severe mutations also conditioned the largest increase in transcripts. The analysis was extended by monitoring the transcripts of the genes, shrunken1 (sh1, structural gene for Suc synthase), sh2, bt2, waxy1 (wx1, structural gene for starch synthase), and those of the large and small zeins in isogenic maize lines at 14, 22, and 30 d postpollination. Endosperms were wild type for all of these genes or contained sh1-, sh2-, bt1-, bt2-, opaque2 (o2-), or amylose-extender1 (ae1-) dull1 (du1-) wx1- mutations. Transcripts increased continually throughout kernel development in the mutants relative to the standard W64A used. Variation in the amount of Suc entering the developing seed also altered transcript amounts. The results indicate that starch and protein biosynthetic genes act in a concerted manner, and both are sensitive to mutationally induced differences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhave M. R., Lawrence S., Barton C., Hannah L. C. Identification and molecular characterization of shrunken-2 cDNA clones of maize. Plant Cell. 1990 Jun;2(6):581–588. doi: 10.1105/tpc.2.6.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyer C. D., Preiss J. Multiple forms of starch branching enzyme of maize: evidence for independent genetic control. Biochem Biophys Res Commun. 1978 Jan 13;80(1):169–175. doi: 10.1016/0006-291x(78)91119-1. [DOI] [PubMed] [Google Scholar]
  3. Creech R G. Genetic Control of Carbohydrate Synthesis in Maize Endosperm. Genetics. 1965 Dec;52(6):1175–1186. doi: 10.1093/genetics/52.6.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Giroux M. J., Hannah L. C. ADP-glucose pyrophosphorylase in shrunken-2 and brittle-2 mutants of maize. Mol Gen Genet. 1994 May 25;243(4):400–408. doi: 10.1007/BF00280470. [DOI] [PubMed] [Google Scholar]
  5. Koch K. E., Nolte K. D., Duke E. R., McCarty D. R., Avigne W. T. Sugar Levels Modulate Differential Expression of Maize Sucrose Synthase Genes. Plant Cell. 1992 Jan;4(1):59–69. doi: 10.1105/tpc.4.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. MERTZ E. T., BATES L. S., NELSON O. E. MUTANT GENE THAT CHANGES PROTEIN COMPOSITION AND INCREASES LYSINE CONTENT OF MAIZE ENDOSPERM. Science. 1964 Jul 17;145(3629):279–280. doi: 10.1126/science.145.3629.279. [DOI] [PubMed] [Google Scholar]
  8. McCarty D. R., Shaw J. R., Hannah L. C. The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9099–9103. doi: 10.1073/pnas.83.23.9099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Müller-Röber B. T., Kossmann J., Hannah L. C., Willmitzer L., Sonnewald U. One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol Gen Genet. 1990 Oct;224(1):136–146. doi: 10.1007/BF00259460. [DOI] [PubMed] [Google Scholar]
  10. Prioul J. L., Jeannette E., Reyss A., Grégory N., Giroux M., Hannah L. C., Causse M. Expression of ADP-glucose pyrophosphorylase in maize (Zea mays L.) grain and source leaf during grain filling. Plant Physiol. 1994 Jan;104(1):179–187. doi: 10.1104/pp.104.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sheen J. Metabolic repression of transcription in higher plants. Plant Cell. 1990 Oct;2(10):1027–1038. doi: 10.1105/tpc.2.10.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sullivan T. D., Strelow L. I., Illingworth C. A., Phillips R. L., Nelson O. E., Jr Analysis of maize brittle-1 alleles and a defective Suppressor-mutator-induced mutable allele. Plant Cell. 1991 Dec;3(12):1337–1348. doi: 10.1105/tpc.3.12.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Taliercio E. W., Chourey P. S. Post-transcriptional control of sucrose synthase expression in anaerobic seedlings of maize. Plant Physiol. 1989 Aug;90(4):1359–1364. doi: 10.1104/pp.90.4.1359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tsai C. Y., Larkins B. A., Glover D. V. Interaction of the opaque-2 gene with starch-forming mutant genes on the synthesis of zein in maize endosperm. Biochem Genet. 1978 Oct;16(9-10):883–896. doi: 10.1007/BF00483740. [DOI] [PubMed] [Google Scholar]
  15. Walling L., Drews G. N., Goldberg R. B. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123–2127. doi: 10.1073/pnas.83.7.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]

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