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. 1994 Nov;106(3):829–839. doi: 10.1104/pp.106.3.829

Assembly of the Light-Harvesting Complexes (LHCs) of Photosystem II (Monomeric LHC IIb Complexes Are Intermediates in the Formation of Oligomeric LHC IIb Complexes).

B W Dreyfuss 1, J P Thornber 1
PMCID: PMC159606  PMID: 12232371

Abstract

The light-induced assembly of light-harvesting complex (LHC) II has been followed during the biogenesis of the plastid. Seedlings grown in intermittent light (IML) accumulate only small amounts of chlorophyll b. The minor LHC II apoproteins are present; however, the apoprotein levels of the major LHC II complex, LHC IIb, are severely depressed after exposure to IML. The levels of all LHC II apoproteins increase rapidly upon exposure to continuous illumination. The 25-kD, type 3 LHC IIb subunit appears to be more abundant during the early hours of greening in relation to its level in mature thylakoids. The LHC IIb apoproteins are initially associated with pigments to form monomeric pigment-protein complexes. The abundance of monomeric LHC IIb complexes gradually decreases during exposure to continuous light and a concomitant increase occurs in the amount of the trimeric and higher-order oligomeric forms. Pulse-chase experiments verify that labeled LHC IIb monomeric complexes are intermediates in the formation of trimeric and higher-order oligomeric LHC IIb-pigmented complexes. Therefore, the assembly of LHC II occurs via the initial pigmentation of the apoproteins to form monomeric complexes and proceeds in a sequential manner.

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Selected References

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  1. Argyroudi-Akoyunoglou J. H., Akoyunoglou G. Photoinduced changes in the chlorophyll a to chlorophyll B ratio in young bean plants. Plant Physiol. 1970 Aug;46(2):247–249. doi: 10.1104/pp.46.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armond P. A., Arntzen C. J., Briantais J. M., Vernotte C. Differentiation of chloroplast lamellae. Light harvesting efficiency and grana development. Arch Biochem Biophys. 1976 Jul;175(1):54–63. doi: 10.1016/0003-9861(76)90484-7. [DOI] [PubMed] [Google Scholar]
  3. Bassi R., Høyer-Hansen G., Barbato R., Giacometti G. M., Simpson D. J. Chlorophyll-proteins of the photosystem II antenna system. J Biol Chem. 1987 Sep 25;262(27):13333–13341. [PubMed] [Google Scholar]
  4. Bellemare G., Bartlett S. G., Chua N. H. Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley. J Biol Chem. 1982 Jul 10;257(13):7762–7767. [PubMed] [Google Scholar]
  5. Cuming A. C., Bennett J. Biosynthesis of the light-harvesting chlorophyll a/b protein. Control of messenger RNA activity by light. Eur J Biochem. 1981 Aug;118(1):71–80. doi: 10.1111/j.1432-1033.1981.tb05487.x. [DOI] [PubMed] [Google Scholar]
  6. Darr S. C., Somerville S. C., Arntzen C. J. Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J Cell Biol. 1986 Sep;103(3):733–740. doi: 10.1083/jcb.103.3.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dreyfuss B. W., Thornber J. P. Organization of the Light-Harvesting Complex of Photosystem I and Its Assembly during Plastid Development. Plant Physiol. 1994 Nov;106(3):841–848. doi: 10.1104/pp.106.3.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Greene B. A., Allred D. R., Morishige D. T., Staehelin L. A. Hierarchical Response of Light Harvesting Chlorophyll-Proteins in a Light-Sensitive Chlorophyll b-Deficient Mutant of Maize. Plant Physiol. 1988 Jun;87(2):357–364. doi: 10.1104/pp.87.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huner N. P., Krol M., Williams J. P., Maissan E., Low P. S., Roberts D., Thompson J. E. Low Temperature Development Induces a Specific Decrease in trans-Delta-Hexadecenoic Acid Content which Influences LHCII Organization. Plant Physiol. 1987 May;84(1):12–18. doi: 10.1104/pp.84.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jansson S., Selstam E., Gustafsson P. The rapidly phosphorylated 25 kDa polypeptide of the light- harvesting complex of photosystem II is encoded by the type 2 cab-II genes. Biochim Biophys Acta. 1990 Aug 30;1019(2):110–114. doi: 10.1016/0005-2728(90)90130-v. [DOI] [PubMed] [Google Scholar]
  11. Jansson S. The light-harvesting chlorophyll a/b-binding proteins. Biochim Biophys Acta. 1994 Feb 8;1184(1):1–19. doi: 10.1016/0005-2728(94)90148-1. [DOI] [PubMed] [Google Scholar]
  12. Kühlbrandt W., Wang D. N., Fujiyoshi Y. Atomic model of plant light-harvesting complex by electron crystallography. Nature. 1994 Feb 17;367(6464):614–621. doi: 10.1038/367614a0. [DOI] [PubMed] [Google Scholar]
  13. Kühlbrandt W., Wang D. N. Three-dimensional structure of plant light-harvesting complex determined by electron crystallography. Nature. 1991 Mar 14;350(6314):130–134. doi: 10.1038/350130a0. [DOI] [PubMed] [Google Scholar]
  14. McCourt P., Browse J., Watson J., Arntzen C. J., Somerville C. R. Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid. Plant Physiol. 1985 Aug;78(4):853–858. doi: 10.1104/pp.78.4.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morishige D. T., Thornber J. P. Identification and Analysis of a Barley cDNA Clone Encoding the 31-Kilodalton LHC IIa (CP29) Apoprotein of the Light-Harvesting Antenna Complex of Photosystem II. Plant Physiol. 1992 Jan;98(1):238–245. doi: 10.1104/pp.98.1.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Paulsen H., Finkenzeller B., Kühlein N. Pigments induce folding of light-harvesting chlorophyll a/b-binding protein. Eur J Biochem. 1993 Aug 1;215(3):809–816. doi: 10.1111/j.1432-1033.1993.tb18096.x. [DOI] [PubMed] [Google Scholar]
  17. Peter G. F., Thornber J. P. Biochemical composition and organization of higher plant photosystem II light-harvesting pigment-proteins. J Biol Chem. 1991 Sep 5;266(25):16745–16754. [PubMed] [Google Scholar]
  18. Plumley F. G., Schmidt G. W. Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer. Proc Natl Acad Sci U S A. 1987 Jan;84(1):146–150. doi: 10.1073/pnas.84.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sigrist M., Staehelin L. A. Appearance of type 1, 2, and 3 light-harvesting complex II and light-harvesting complex I proteins during light-induced greening of barley (Hordeum vulgare) etioplasts. Plant Physiol. 1994 Jan;104(1):135–145. doi: 10.1104/pp.104.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thornber J. P., Peter G. F., Morishige D. T., Gómez S., Anandan S., Welty B. A., Lee A., Kerfeld C., Takeuchi T., Preiss S. Light harvesting in photosystems I and II. Biochem Soc Trans. 1993 Feb;21(1):15–18. doi: 10.1042/bst0210015. [DOI] [PubMed] [Google Scholar]

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