Abstract
The purpose of this study was to demonstrate the metabolism of gibberellin A20 (GA20) to gibberellin A1 (GA1) by tall and mutant shoots of rice (Oryza sativa L.) and Arabidopsis thaliana (L.) Heynh. The data show that the tall and dx mutant of rice and the tall and ga5 mutant of Arabidopsis metabolize GA20 to GA1. The data also show that the dy mutant of rice and the ga4 mutant of Arabidopsis block the metabolism of GA20 to GA1. [17-13C,3H]GA20 was fed to tall and the dwarf mutants, dx and dy, of rice and tall and the dwarf mutants, ga5 and ga4, of Arabidopsis. The metabolites were analyzed by high-performance liquid chromatography and full-scan gas chromatography-mass spectrometry together with Kovats retention index data. For rice, the metabolite [13C]GA, was identified from tall and dx seedlings; [13C]GA1 was not identified from the dy seedlings. [13C]GA29 was identified from tall, dx, and dy seedlings. For Arabidopsis, the metabolite [13C]GA1 was identified from tall, ga5, and ga4 plants. The amount of [13C]GA1 from ga4 plants was less than 15% of that obtained from tall and ga5 plants. [13C]GA29 was identified from tall, ga5, and ga4 plants. [13C]GA5 and [13C]GA3 were not identified from any of the six types of plant material.
Full Text
The Full Text of this article is available as a PDF (503.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Fujioka S., Yamane H., Spray C. R., Phinney B. O., Gaskin P., Macmillan J., Takahashi N. Gibberellin A(3) Is Biosynthesized from Gibberellin A(20) via Gibberellin A(5) in Shoots of Zea mays L. Plant Physiol. 1990 Sep;94(1):127–131. doi: 10.1104/pp.94.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kobayashi M., Gaskin P., Spray C. R., Suzuki Y., Phinney B. O., MacMillan J. Metabolism and Biological Activity of Gibberellin A4 in Vegetative Shoots of Zea mays, Oryza sativa, and Arabidopsis thaliana. Plant Physiol. 1993 Jun;102(2):379–386. doi: 10.1104/pp.102.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Talon M., Koornneef M., Zeevaart J. A. Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7983–7987. doi: 10.1073/pnas.87.20.7983. [DOI] [PMC free article] [PubMed] [Google Scholar]