Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1994 Dec;106(4):1497–1501. doi: 10.1104/pp.106.4.1497

The 30-kilodalton protein present in purified fusicoccin receptor preparations is a 14-3-3-like protein.

M Marra 1, M R Fullone 1, V Fogliano 1, J Pen 1, M Mattei 1, S Masi 1, P Aducci 1
PMCID: PMC159690  PMID: 7846161

Abstract

We have recently reported on the purification of the fusicoccin (FC) receptor from corn (Zea mays L.) and its identification by photoaffinity labeling (P. Aducci, A. Ballio, V. Fogliano, M.R. Fullone, M. Marra, N. Proietti [1993] Eur J Biochem 214: 339-345). Pure preparations of FC receptors, obtained under nondenaturing conditions, showed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis two doublets of proteins with apparent molecular masses of 30 and 90 kD. In the present paper we describe the isolation and identification of the primary structure of the 30-kD doublet proteins. Sequencing studies of peptides resulting from the digestion of the 30-kD protein showed a full identity with a 14-3-3-like protein from corn, named GF14. The 14-3-3 family is a class of proteins that is widely distributed in eukaryotes and is known to play various regulatory roles. The 30-kD protein has been immunologically identified by specific antibodies prepared against a synthetic peptide based on the determined amino acid sequence. A similar protein is recognized in partially purified FC receptor preparations from bean and spinach leaves.

Full Text

The Full Text of this article is available as a PDF (1.5 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aducci P., Ballio A., Blein J. P., Fullone M. R., Rossignol M., Scalla R. Functional reconstitution of a proton-translocating system responsive to fusicoccin. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7849–7851. doi: 10.1073/pnas.85.21.7849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aducci P., Ballio A., Fogliano V., Fullone M. R., Marra M., Proietti N. Purification and photoaffinity labeling of fusicoccin receptors from maize. Eur J Biochem. 1993 May 15;214(1):339–345. doi: 10.1111/j.1432-1033.1993.tb17929.x. [DOI] [PubMed] [Google Scholar]
  3. Aitken A., Ellis C. A., Harris A., Sellers L. A., Toker A. Kinase and neurotransmitters. Nature. 1990 Apr 12;344(6267):594–594. doi: 10.1038/344594a0. [DOI] [PubMed] [Google Scholar]
  4. Ballio A., Carilli A., Santurbano B., Tuttobello L. Produzione di fusicoccina in scala pilota. Ann Ist Super Sanita. 1968;4(3):317–332. [PubMed] [Google Scholar]
  5. Hirsch S., Aitken A., Bertsch U., Soll J. A plant homologue to mammalian brain 14-3-3 protein and protein kinase C inhibitor. FEBS Lett. 1992 Jan 20;296(2):222–224. doi: 10.1016/0014-5793(92)80384-s. [DOI] [PubMed] [Google Scholar]
  6. Ichimura T., Isobe T., Okuyama T., Takahashi N., Araki K., Kuwano R., Takahashi Y. Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7084–7088. doi: 10.1073/pnas.85.19.7084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johansson F., Sommarin M., Larsson C. Fusicoccin Activates the Plasma Membrane H+-ATPase by a Mechanism Involving the C-Terminal Inhibitory Domain. Plant Cell. 1993 Mar;5(3):321–327. doi: 10.1105/tpc.5.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Mochly-Rosen D., Khaner H., Lopez J., Smith B. L. Intracellular receptors for activated protein kinase C. Identification of a binding site for the enzyme. J Biol Chem. 1991 Aug 15;266(23):14866–14868. [PubMed] [Google Scholar]
  10. Oecking C., Weiler E. W. Characterization and purification of the fusicoccin-binding complex from plasma membranes of Commelina communis. Eur J Biochem. 1991 Aug 1;199(3):685–689. doi: 10.1111/j.1432-1033.1991.tb16171.x. [DOI] [PubMed] [Google Scholar]
  11. Rasi-Caldognov F., Pugliarello M. C., Olivari C., De Michelis M. I. Controlled Proteolysis Mimics the Effect of Fusicoccin on the Plasma Membrane H+-ATPase. Plant Physiol. 1993 Oct;103(2):391–398. doi: 10.1104/pp.103.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. de Boer A. H., Watson B. A., Cleland R. E. Purification and identification of the fusicoccin binding protein from oat root plasma membrane. Plant Physiol. 1989;89:250–259. doi: 10.1104/pp.89.1.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Vetten N. C., Lu G., Feri R. J. A maize protein associated with the G-box binding complex has homology to brain regulatory proteins. Plant Cell. 1992 Oct;4(10):1295–1307. doi: 10.1105/tpc.4.10.1295. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES