Abstract
During the anaerobic germination of rice (Oryza sativa L.), nitrate is translocated from the caryopsis and assimilated into the coleoptile (R. Reggiani, M. Mattana, N. Aurisano, A. Bertani [1993] Plant Cell Physiol 34: 379-383). Using antibodies against nitrate and nitrite reductases, proteins with the expected molecular mass were recognized by western blot analysis in extracts from 8-d-old rice coleoptiles. Both enzymes are de novo synthesized in 6- to 8-d-old seedlings, as shown by immunoprecipitation of radiolabeled proteins from young plants grown in the presence of [35S]methio-nine. The anaerobic synthesis of both enzymes was enhanced by the addition of 5 mM KNO3. The effect of exogenous nitrate on the expression of the corresponding genes in anaerobic rice coleoptiles was revealed by the analysis of their transcripts. The importance of the expression of these enzymes during the anaerobic development of rice seedlings is discussed.
Full Text
The Full Text of this article is available as a PDF (849.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caboche M., Rouzé P. Nitrate reductase: a target for molecular and cellular studies in higher plants. Trends Genet. 1990 Jun;6(6):187–192. doi: 10.1016/0168-9525(90)90175-6. [DOI] [PubMed] [Google Scholar]
- Calza R, Huttner E, Vincentz M, Rouzé P, Galangau F, Vaucheret H, Chérel I, Meyer C, Kronenberger J, Caboche M. Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductases from higher plants. Mol Gen Genet. 1987 Oct;209(3):552–562. doi: 10.1007/BF00331162. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
- Ferrari T. E., Varner J. E. Intact tissue assay for nitrite reductase in barley aleurone layers. Plant Physiol. 1971 Jun;47(6):790–794. doi: 10.1104/pp.47.6.790. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferrari T. E., Yoder O. C., Filner P. Anaerobic nitrite production by plant cells and tissues: evidence for two nitrate pools. Plant Physiol. 1973 Mar;51(3):423–431. doi: 10.1104/pp.51.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gething M. J., McCammon K., Sambrook J. Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport. Cell. 1986 Sep 12;46(6):939–950. doi: 10.1016/0092-8674(86)90076-0. [DOI] [PubMed] [Google Scholar]
- Jaworski E. G. Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun. 1971 Jun 18;43(6):1274–1279. doi: 10.1016/s0006-291x(71)80010-4. [DOI] [PubMed] [Google Scholar]
- Sachs M. M., Freeling M., Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. doi: 10.1016/0092-8674(80)90322-0. [DOI] [PubMed] [Google Scholar]
- Somers D. A., Kuo T. M., Kleinhofs A., Warner R. L., Oaks A. Synthesis and degradation of barley nitrate reductase. Plant Physiol. 1983 Aug;72(4):949–952. doi: 10.1104/pp.72.4.949. [DOI] [PMC free article] [PubMed] [Google Scholar]