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. 1989 Apr;1(4):471–478. doi: 10.1105/tpc.1.4.471

The Pea rbcS-3A Promoter Mediates Light Responsiveness but not Organ Specificity.

C Kuhlemeier 1, G Strittmatter 1, K Ward 1, N H Chua 1
PMCID: PMC159778  PMID: 12359895

Abstract

The expression of the pea rbcS-3A gene is responsive to light and restricted to chloroplast-containing cells. Previously, we identified transcriptional elements between -410 and -50 relative to the transcription start site as being sufficient for this regulated expression. Here we investigate the role of the promoter region (-50 to +15) in rbcS-3A expression. We demonstrate an interaction between the promoter and upstream sequences located between -189 and -166, which is important for high level expression. In addition we show that, when the rbcS promoter is driven by a weak test enhancer, it can confer light-responsive but not organ-specific expression upon a bacterial reporter gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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