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. 1989 May;1(5):523–532. doi: 10.1105/tpc.1.5.523

Molecular Analysis of viviparous-1: An Abscisic Acid-Insensitive Mutant of Maize.

D R McCarty 1, C B Carson 1, P S Stinard 1, D S Robertson 1
PMCID: PMC159786  PMID: 12359900

Abstract

The viviparous-1 (vp1) gene in maize controls multiple developmental responses associated with the maturation phase of seed formation. Most notably, mutant embryos have reduced sensitivity to the hormone abscisic acid, resulting in precocious germination, and blocked anthocyanin synthesis in aleurone and embryo tissues. The Vp1 locus was cloned by transposon tagging, using the Robertson's Mutator element present in the vp1-mum1 mutant allele. Detection of DNA rearrangements in several spontaneous and transposable element-induced mutant vp1 alleles, including a partial deletion of the locus, confirmed the identity of the clone. The Vp1 gene encodes a 2500-nucleotide mRNA that is expressed specifically in embryo and endosperm tissues of the developing seed. This transcript is absent in seed tissues of vp1 mutant stocks. Expression of C1, a regulatory gene for the anthocyanin pathway, is selectively blocked at the mRNA level in vp1 mutant seed tissues, indicating the Vp1 may control the anthocyanin pathway by regulating C1. We suggest that the Vp1 gene product functions to potentiate multiple signal transduction pathways in specific seed tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashman R B. Stippled Aleurone in Maize. Genetics. 1960 Jan;45(1):19–34. doi: 10.1093/genetics/45.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barker R. F., Thompson D. V., Talbot D. R., Swanson J., Bennetzen J. L. Nucleotide sequence of the maize transposable element Mul. Nucleic Acids Res. 1984 Aug 10;12(15):5955–5967. doi: 10.1093/nar/12.15.5955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennetzen J. L. Transposable element Mu1 is found in multiple copies only in Robertson's Mutator maize lines. J Mol Appl Genet. 1984;2(6):519–524. [PubMed] [Google Scholar]
  4. Chen C. H., Oishi K. K., Kloeckener-Gruissem B., Freeling M. Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics. 1987 Jul;116(3):469–477. doi: 10.1093/genetics/116.3.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chen S. M., Coe E. H., Jr Control of anthocyanin synthesis by the C locus in maize. Biochem Genet. 1977 Apr;15(3-4):333–346. doi: 10.1007/BF00484464. [DOI] [PubMed] [Google Scholar]
  6. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cone K. C., Burr F. A., Burr B. Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9631–9635. doi: 10.1073/pnas.83.24.9631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dennis E. S., Gerlach W. L., Pryor A. J., Bennetzen J. L., Inglis A., Llewellyn D., Sachs M. M., Ferl R. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984 May 11;12(9):3983–4000. doi: 10.1093/nar/12.9.3983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dooner H. K., Nelson O. E. Interaction among C, R and Vp in the Control of the Bz Glucosyltransferase during Endosperm Development in Maize. Genetics. 1979 Feb;91(2):309–315. doi: 10.1093/genetics/91.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Freeling M., Bennett D. C. Maize Adh1. Annu Rev Genet. 1985;19:297–323. doi: 10.1146/annurev.ge.19.120185.001501. [DOI] [PubMed] [Google Scholar]
  11. McCarty D. R., Shaw J. R., Hannah L. C. The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9099–9103. doi: 10.1073/pnas.83.23.9099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Paz-Ares J., Wienand U., Peterson P. A., Saedler H. Molecular cloning of the c locus of Zea mays: a locus regulating the anthocyanin pathway. EMBO J. 1986 May;5(5):829–833. doi: 10.1002/j.1460-2075.1986.tb04291.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robertson D. S. The Genetics of Vivipary in Maize. Genetics. 1955 Sep;40(5):745–760. doi: 10.1093/genetics/40.5.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Robertson D. S. The timing of mu activity in maize. Genetics. 1980 Apr;94(4):969–978. doi: 10.1093/genetics/94.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor L. P., Walbot V. Isolation and characterization of a 1.7-kb transposable element from a mutator line of maize. Genetics. 1987 Oct;117(2):297–307. doi: 10.1093/genetics/117.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wilson G. F., Rhodes A. M., Dickinson D. B. Some Physiological Effects of Viviparous Genes vp(1) and vp(5) on Developing Maize Kernels. Plant Physiol. 1973 Oct;52(4):350–356. doi: 10.1104/pp.52.4.350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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