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. 1989 Jul;1(7):681–690. doi: 10.1105/tpc.1.7.681

Characterization of a single copy gene encoding ferredoxin I from pea.

R C Elliott 1, T J Pedersen 1, B Fristensky 1, M J White 1, L F Dickey 1, W F Thompson 1
PMCID: PMC159804  PMID: 2535518

Abstract

We have isolated, mapped, and sequenced a genomic clone containing the ferredoxin I (Fed-1) gene from Pisum sativum. The gene is present as a single copy per haploid genome. It has no introns, and it specifies a 753-nucleotide transcript encoding a 149-amino acid protein including a 52-residue transit peptide. Upstream sequences from Fed-1 contain several elements with similarity to transcriptional regulatory elements from RbcS and Cab genes, and gel mobility shift assays show that nuclear extracts from light-grown pea leaves contain one or more DNA binding activities specific for Fed-1 5'-flanking sequences. RbcS and Cab regulatory sequences are only weak competitors for this binding, however, and the RbcS and Cab similarities mostly lie outside of the region essential for binding. These data are discussed in terms of previously observed physiological differences between the light responses of Fed-1 and other genes.

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Selected References

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  1. Arnon D. I. The discovery of ferredoxin: the photosynthetic path. Trends Biochem Sci. 1988 Jan;13(1):30–33. doi: 10.1016/0968-0004(88)90016-3. [DOI] [PubMed] [Google Scholar]
  2. Castresana C., Garcia-Luque I., Alonso E., Malik V. S., Cashmore A. R. Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia. EMBO J. 1988 Jul;7(7):1929–1936. doi: 10.1002/j.1460-2075.1988.tb03030.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elliott R. C., Dickey L. F., White M. J., Thompson W. F. cis-Acting Elements for Light Regulation of Pea Ferredoxin I Gene Expression Are Located within Transcribed Sequences. Plant Cell. 1989 Jul;1(7):691–698. doi: 10.1105/tpc.1.7.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fluhr Robert, Moses Phyllis, Morelli Giorgio, Coruzzi Gloria, Chua Nam-Hai. Expression dynamics of the pea rbcS multigene family and organ distribution of the transcripts. EMBO J. 1986 Sep;5(9):2063–2071. doi: 10.1002/j.1460-2075.1986.tb04467.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fried M., Crothers D. M. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 1981 Dec 11;9(23):6505–6525. doi: 10.1093/nar/9.23.6505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Geliebter J., Zeff R. A., Melvold R. W., Nathenson S. G. Mitotic recombination in germ cells generated two major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis: Kbm9 and Kbm6. Proc Natl Acad Sci U S A. 1986 May;83(10):3371–3375. doi: 10.1073/pnas.83.10.3371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gil A., Proudfoot N. J. Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987 May 8;49(3):399–406. doi: 10.1016/0092-8674(87)90292-3. [DOI] [PubMed] [Google Scholar]
  8. Giuliano G., Pichersky E., Malik V. S., Timko M. P., Scolnik P. A., Cashmore A. R. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089–7093. doi: 10.1073/pnas.85.19.7089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grabowski P. J., Padgett R. A., Sharp P. A. Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell. 1984 Jun;37(2):415–427. doi: 10.1016/0092-8674(84)90372-6. [DOI] [PubMed] [Google Scholar]
  10. Green P. J., Kay S. A., Chua N. H. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J. 1987 Sep;6(9):2543–2549. doi: 10.1002/j.1460-2075.1987.tb02542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green P. J., Yong M. H., Cuozzo M., Kano-Murakami Y., Silverstein P., Chua N. H. Binding site requirements for pea nuclear protein factor GT-1 correlate with sequences required for light-dependent transcriptional activation of the rbcS-3A gene. EMBO J. 1988 Dec 20;7(13):4035–4044. doi: 10.1002/j.1460-2075.1988.tb03297.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grob U., Stüber K. Discrimination of phytochrome dependent light inducible from non-light inducible plant genes. Prediction of a common light-responsive element (LRE) in phytochrome dependent light inducible plant genes. Nucleic Acids Res. 1987 Dec 10;15(23):9957–9973. doi: 10.1093/nar/15.23.9957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hunt T. Controlling mRNA lifespan. Nature. 1988 Aug 18;334(6183):567–568. doi: 10.1038/334567a0. [DOI] [PubMed] [Google Scholar]
  14. Jensen E. Ø, Marcker K. A., Schell J., Bruijn F. J. Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghaemoglobin Ibc(3) 5' upstream region. EMBO J. 1988 May;7(5):1265–1271. doi: 10.1002/j.1460-2075.1988.tb02940.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Joshi C. P. Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucleic Acids Res. 1987 Dec 10;15(23):9627–9640. doi: 10.1093/nar/15.23.9627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaufman L. S., Briggs W. R., Thompson W. F. Phytochrome control of specific mRNA levels in developing pea buds : the presence of both very low fluence and low fluence responses. Plant Physiol. 1985 Jun;78(2):388–393. doi: 10.1104/pp.78.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Keegstra K., Bauerle C. Targeting of proteins into chloroplasts. Bioessays. 1988 Jul;9(1):15–19. doi: 10.1002/bies.950090105. [DOI] [PubMed] [Google Scholar]
  18. Krieg P. A., Melton D. A. In vitro RNA synthesis with SP6 RNA polymerase. Methods Enzymol. 1987;155:397–415. doi: 10.1016/0076-6879(87)55027-3. [DOI] [PubMed] [Google Scholar]
  19. Kuhlemeier C., Fluhr R., Green P. J., Chua N. H. Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements. Genes Dev. 1987 May;1(3):247–255. doi: 10.1101/gad.1.3.247. [DOI] [PubMed] [Google Scholar]
  20. Loenen W. A., Brammar W. J. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene. 1980 Aug;10(3):249–259. doi: 10.1016/0378-1119(80)90054-2. [DOI] [PubMed] [Google Scholar]
  21. McKnight S. L., Kingsbury R. Transcriptional control signals of a eukaryotic protein-coding gene. Science. 1982 Jul 23;217(4557):316–324. doi: 10.1126/science.6283634. [DOI] [PubMed] [Google Scholar]
  22. Nagai J., Bloch K. Enzymatic desaturation of stearyl acyl carrier protein. J Biol Chem. 1966 Apr 25;241(8):1925–1927. [PubMed] [Google Scholar]
  23. Ramírez J. M., Del Campo F. F., Paneque A., Losada M. Ferredoxin-nitrite reductase from spinach. Biochim Biophys Acta. 1966 Apr 12;118(1):58–71. doi: 10.1016/s0926-6593(66)80144-3. [DOI] [PubMed] [Google Scholar]
  24. Sakihama N., Shin M. Evidence from high-pressure liquid chromatography for the existence of two ferredoxins in plants. Arch Biochem Biophys. 1987 Aug 1;256(2):430–434. doi: 10.1016/0003-9861(87)90599-6. [DOI] [PubMed] [Google Scholar]
  25. Schmidt A., Trebst A. The mechanism of photosynthetic sulfate reduction by isolated chloroplasts. Biochim Biophys Acta. 1969 Aug 5;180(3):529–535. doi: 10.1016/0005-2728(69)90031-0. [DOI] [PubMed] [Google Scholar]
  26. Smeekens S., van Binsbergen J., Weisbeek P. The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone. Nucleic Acids Res. 1985 May 10;13(9):3179–3194. doi: 10.1093/nar/13.9.3179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zinn K., DiMaio D., Maniatis T. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Cell. 1983 Oct;34(3):865–879. doi: 10.1016/0092-8674(83)90544-5. [DOI] [PubMed] [Google Scholar]

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