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. 1989 Aug;1(8):747–755. doi: 10.1105/tpc.1.8.747

Pectic Cell Wall Fragments Regulate Tobacco Thin-Cell-Layer Explant Morphogenesis.

S Eberhard 1, N Doubrava 1, V Marfa 1, D Mohnen 1, A Southwick 1, A Darvill 1, P Albersheim 1
PMCID: PMC159812  PMID: 12359909

Abstract

Pectic fragments of cell wall polysaccharides, released from the walls of suspension-cultured sycamore cells by treatment with endopolygalacturonase, were tested for morphogenesis-regulating activity in a modified tobacco thin-cell-layer explant (TCL) bioassay (D. Mohnen, S. Eberhard, V. Marfa, N. Doubrava, P. Toubart, D. J. Gollin, T.A. Gruber, W. Nuri, P. Albersheim, and A. Darvill, manuscript submitted). The pectic fragments inhibited the formation of roots on TCLs grown on a root-inducing medium containing 15 micromolar indole-3-butyric acid and 0.5 micromolar kinetin. Addition of the pectic fragments to a root-inducing medium containing 7 micromolar indole-3-butyric acid and 0.15 micromolar kinetin caused roots to form on the basal end of TCLs. TCLs cultured on this medium in the absence of added pectic fragments formed roots along their entire length. The pectic fragments induced polar tissue enlargement and the formation of flowers on TCLs cultured on transition medium. The flower-inducing activity was stable to heat treatment and proteolytic digestion. Pectic fragments isolated from the walls of suspension-cultured tobacco cells were as effective as those from the walls of sycamore cells in inducing de novo flower formation in the TCLs. These results support the hypothesis that oligosaccharins from plant cell walls regulate morphogenesis.

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Selected References

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  1. Cervone F., De Lorenzo G., Degrà L., Salvi G. Elicitation of Necrosis in Vigna unguiculata Walp. by Homogeneous Aspergillus niger Endo-Polygalacturonase and by alpha-d-Galacturonate Oligomers. Plant Physiol. 1987 Nov;85(3):626–630. doi: 10.1104/pp.85.3.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chambers R. E., Clamp J. R. An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials. Biochem J. 1971 Dec;125(4):1009–1018. doi: 10.1042/bj1251009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Darvill A. G., McNeil M., Albersheim P. Structure of Plant Cell Walls: VIII. A New Pectic Polysaccharide. Plant Physiol. 1978 Sep;62(3):418–422. doi: 10.1104/pp.62.3.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. English P. D., Maglothin A., Keegstra K., Albersheim P. A Cell Wall-degrading Endopolygalacturonase Secreted by Colletotrichum lindemuthianum. Plant Physiol. 1972 Mar;49(3):293–298. doi: 10.1104/pp.49.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hayashi T., Yoshida K. Cell expansion and single-cell separation induced by colchicine in suspension-cultured soybean cells. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2618–2622. doi: 10.1073/pnas.85.8.2618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hillson T. D., Lamotte C. E. In vitro formation and development of floral buds on tobacco stem explants: effects of kinetin and other factors. Plant Physiol. 1977 Dec;60(6):881–884. doi: 10.1104/pp.60.6.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee S. C., West C. A. Polygalacturonase from Rhizopus stolonifer, an Elicitor of Casbene Synthetase Activity in Castor Bean (Ricinus communis L.) Seedlings. Plant Physiol. 1981 Apr;67(4):633–639. doi: 10.1104/pp.67.4.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacKenzie S. L., Tenaschuk D. Gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters of amino acids. J Chromatogr. 1974 Oct 9;97(1):19–24. doi: 10.1016/s0021-9673(01)97579-x. [DOI] [PubMed] [Google Scholar]
  9. McDougall G. J., Fry S. C. Structure-activity relationships for xyloglucan oligosaccharides with antiauxin activity. Plant Physiol. 1989 Mar;89(3):883–887. doi: 10.1104/pp.89.3.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ragster L. V., Chrispeels M. J. Azocoll-digesting Proteinases in Soybean Leaves: Characteristics and Changes during Leaf Maturation and Senescence. Plant Physiol. 1979 Nov;64(5):857–862. doi: 10.1104/pp.64.5.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ryan C. A. Oligosaccharide signalling in plants. Annu Rev Cell Biol. 1987;3:295–317. doi: 10.1146/annurev.cb.03.110187.001455. [DOI] [PubMed] [Google Scholar]
  12. Talmadge K. W., Keegstra K., Bauer W. D., Albersheim P. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides. Plant Physiol. 1973 Jan;51(1):158–173. doi: 10.1104/pp.51.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wardell W. L., Skoog F. Flower formation in excised tobacco stem segments; I. Methodology and effects of plant hormones. Plant Physiol. 1969 Oct;44(10):1402–1406. doi: 10.1104/pp.44.10.1402. [DOI] [PMC free article] [PubMed] [Google Scholar]

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