Abstract
We have employed fluorescein and 125l-labeled elicitors of the defense response in soybeans to monitor the cellular distribution and movement of elicitors following their addition to a soybean cell suspension culture. Our results indicate that the macromolecular elicitors first bind to the cell surface and then internalize in a temperature- and energy-dependent endocytotic process. Within a few hours, virtually all of the elicitor is concentrated in the major vacuole or tonoplast of the cell. Nonspecific (control) proteins neither bound to the cell surface nor internalized in parallel assays.
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- Apostol I., Heinstein P. F., Low P. S. Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction. Plant Physiol. 1989 May;90(1):109–116. doi: 10.1104/pp.90.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bulcke M. V., Bauw G., Castresana C., Van Montagu M., Vandekerckhove J. Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: Evidence for a strictly compartmentalized plant defense system. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2673–2677. doi: 10.1073/pnas.86.8.2673. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cosio E. G., Pöpperl H., Schmidt W. E., Ebel J. High-affinity binding of fungal beta-glucan fragments to soybean (Glycine max L.) microsomal fractions and protoplasts. Eur J Biochem. 1988 Aug 1;175(2):309–315. doi: 10.1111/j.1432-1033.1988.tb14198.x. [DOI] [PubMed] [Google Scholar]
- Hicks G. R., Rayle D. L., Lomax T. L. The diageotropica mutant of tomato lacks high specific activity auxin binding sites. Science. 1989 Jul 7;245:52–54. doi: 10.1126/science.245.4913.52. [DOI] [PubMed] [Google Scholar]
- Joachim S., Robinson D. G. Endocytosis of cationic ferritin by bean leaf protoplasts. Eur J Cell Biol. 1984 May;34(1):212–216. [PubMed] [Google Scholar]
- Low P. S., Heinstein P. F. Elicitor stimulation of the defense response in cultured plant cells monitored by fluorescent dyes. Arch Biochem Biophys. 1986 Sep;249(2):472–479. doi: 10.1016/0003-9861(86)90024-x. [DOI] [PubMed] [Google Scholar]
- Schmidt W. E., Ebel J. Specific binding of a fungal glucan phytoalexin elicitor to membrane fractions from soybean Glycine max. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4117–4121. doi: 10.1073/pnas.84.12.4117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp J. K., McNeil M., Albersheim P. The primary structures of one elicitor-active and seven elicitor-inactive hexa(beta-D-glucopyranosyl)-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f. sp. glycinea. J Biol Chem. 1984 Sep 25;259(18):11321–11336. [PubMed] [Google Scholar]
- Wheeler H., Hanchey P. Pinocytosis and membrane dilation in uranyl-treated plant roots. Science. 1971 Jan 8;171(3966):68–71. doi: 10.1126/science.171.3966.68. [DOI] [PubMed] [Google Scholar]
- Yoshikawa M., Keen N. T., Wang M. C. A receptor on soybean membranes for a fungal elicitor of phytoalexin accumulation. Plant Physiol. 1983 Oct;73(2):497–506. doi: 10.1104/pp.73.2.497. [DOI] [PMC free article] [PubMed] [Google Scholar]