Abstract
Progeny from a transformed Arabidopsis plant (produced by the Agrobacterium-mediated seed transformation procedure) were found to be segregating for an altered trichome phenotype. The mutant plants have normal leaf trichomes but completely lack trichomes usually found on the stem. The mutation is tightly linked to a T-DNA insert. Complementation analysis with genetically characterized trichome mutants revealed that the new mutation is an allele of the GL1 locus. The new trichome mutant has been designated gl1-43. DNA gel blot analysis indicated that the insert site contains a complex array of at least four tandemly linked T-DNA units oriented as both direct and inverted repeats. A genomic library, constructed using DNA from gl1-43 plants, was used to clone DNA that flanks the left end of the T-DNA insert. The availability of DNA from the region interrupted by the insert has allowed initial characterization of the wild-type GL1 gene and will permit the eventual cloning and sequencing of this developmentally interesting gene.
Full Text
The Full Text of this article is available as a PDF (2.3 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. doi: 10.1093/nar/12.22.8711. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang C., Bowman J. L., DeJohn A. W., Lander E. S., Meyerowitz E. M. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856–6860. doi: 10.1073/pnas.85.18.6856. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feldmann K. A., Marks M. D., Christianson M. L., Quatrano R. S. A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis. Science. 1989 Mar 10;243(4896):1351–1354. doi: 10.1126/science.243.4896.1351. [DOI] [PubMed] [Google Scholar]
- Hagberg L., Hull R., Hull S., Falkow S., Freter R., Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. doi: 10.1128/iai.40.1.265-272.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hake S., Vollbrecht E., Freeling M. Cloning Knotted, the dominant morphological mutant in maize using Ds2 as a transposon tag. EMBO J. 1989 Jan;8(1):15–22. doi: 10.1002/j.1460-2075.1989.tb03343.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herman P. L., Marks M. D. Trichome Development in Arabidopsis thaliana. II. Isolation and Complementation of the GLABROUS1 Gene. Plant Cell. 1989 Nov;1(11):1051–1055. doi: 10.1105/tpc.1.11.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koornneef M., Dellaert L. W., van der Veen J. H. EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res. 1982 Mar;93(1):109–123. doi: 10.1016/0027-5107(82)90129-4. [DOI] [PubMed] [Google Scholar]
- McCarty D. R., Carson C. B., Stinard P. S., Robertson D. S. Molecular Analysis of viviparous-1: An Abscisic Acid-Insensitive Mutant of Maize. Plant Cell. 1989 May;1(5):523–532. doi: 10.1105/tpc.1.5.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyerowitz E. M., Pruitt R. E. Arabidopsis thaliana and Plant Molecular Genetics. Science. 1985 Sep 20;229(4719):1214–1218. doi: 10.1126/science.229.4719.1214. [DOI] [PubMed] [Google Scholar]
- Weeks D. P., Beerman N., Griffith O. M. A small-scale five-hour procedure for isolating multiple samples of CsCl-purified DNA: application to isolations from mammalian, insect, higher plant, algal, yeast, and bacterial sources. Anal Biochem. 1986 Feb 1;152(2):376–385. doi: 10.1016/0003-2697(86)90423-9. [DOI] [PubMed] [Google Scholar]
- Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]