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. 1990 Jan;2(1):19–27. doi: 10.1105/tpc.2.1.19

Characterization of a rice gene showing organ-specific expression in response to salt stress and drought.

B Claes 1, R Dekeyser 1, R Villarroel 1, M Van den Bulcke 1, G Bauw 1, M Van Montagu 1, A Caplan 1
PMCID: PMC159860  PMID: 2152105

Abstract

Protein changes induced by salinity stress were investigated in the roots of the salt-sensitive rice cultivar Taichung native 1. We found eight proteins to be induced and obtained partial sequences of one with a molecular mass of 15 kilodaltons and an isoelectric point of 5.5. Using an oligonucleotide probe based on this information, a cDNA clone, salT, was selected and found to contain an open reading frame coding for a protein of 145 amino acid residues. salT mRNA accumulates very rapidly in sheaths and roots from mature plants and seedlings upon treatment with Murashige and Skoog salts (1%), air drying, abscisic acid (20 microM), polyethylene glycol (5%), sodium chloride (1%), and potassium chloride (1%). Generally, no induction was seen in the leaf lamina even when the stress should affect all parts of the plant uniformly. The organ-specific response of salT is correlatable with the pattern of Na+ accumulation during salt stress.

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Selected References

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  1. Bauw G., De Loose M., Inzé D., Van Montagu M., Vandekerckhove J. Alterations in the phenotype of plant cells studied by NH(2)-terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4806–4810. doi: 10.1073/pnas.84.14.4806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ben-Hayyim G., Kafkafi U., Ganmore-Neumann R. Role of Internal Potassium in Maintaining Growth of Cultured Citrus Cells on Increasing NaCl and CaCl(2) Concentrations. Plant Physiol. 1987 Oct;85(2):434–439. doi: 10.1104/pp.85.2.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhaskaran S., Smith R. H., Newton R. J. Physiological changes in cultured sorghum cells in response to induced water stress : I. Free proline. Plant Physiol. 1985 Sep;79(1):266–269. doi: 10.1104/pp.79.1.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Binzel M. L., Hasegawa P. M., Rhodes D., Handa S., Handa A. K., Bressan R. A. Solute Accumulation in Tobacco Cells Adapted to NaCl. Plant Physiol. 1987 Aug;84(4):1408–1415. doi: 10.1104/pp.84.4.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Binzel M. L., Hess F. D., Bressan R. A., Hasegawa P. M. Intracellular compartmentation of ions in salt adapted tobacco cells. Plant Physiol. 1988 Feb;86(2):607–614. doi: 10.1104/pp.86.2.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garoff H., Ansorge W. Improvements of DNA sequencing gels. Anal Biochem. 1981 Aug;115(2):450–457. doi: 10.1016/0003-2697(81)90031-2. [DOI] [PubMed] [Google Scholar]
  8. Gómez J., Sánchez-Martínez D., Stiefel V., Rigau J., Puigdomènech P., Pagès M. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature. 1988 Jul 21;334(6179):262–264. doi: 10.1038/334262a0. [DOI] [PubMed] [Google Scholar]
  9. Handa S., Bressan R. A., Handa A. K., Carpita N. C., Hasegawa P. M. Solutes contributing to osmotic adjustment in cultured plant cells adapted to water stress. Plant Physiol. 1983 Nov;73(3):834–843. doi: 10.1104/pp.73.3.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heikkila J. J., Papp J. E., Schultz G. A., Bewley J. D. Induction of heat shock protein messenger RNA in maize mesocotyls by water stress, abscisic Acid, and wounding. Plant Physiol. 1984 Sep;76(1):270–274. doi: 10.1104/pp.76.1.270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hurkman W. J., Tanaka C. K., Dupont F. M. The effects of salt stress on polypeptides in membrane fractions from barley roots. Plant Physiol. 1988 Dec;88(4):1263–1273. doi: 10.1104/pp.88.4.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hurkman W. J., Tanaka C. K. Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol. 1986 Jul;81(3):802–806. doi: 10.1104/pp.81.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jones J. D., Dunsmuir P., Bedbrook J. High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 1985 Oct;4(10):2411–2418. doi: 10.1002/j.1460-2075.1985.tb03949.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kanabus J., Pikaard C. S., Cherry J. H. Heat Shock Proteins in Tobacco Cell Suspension during Growth Cycle. Plant Physiol. 1984 Jul;75(3):639–644. doi: 10.1104/pp.75.3.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Larosa P. C., Hasegawa P. M., Rhodes D., Clithero J. M., Watad A. E., Bressan R. A. Abscisic Acid Stimulated Osmotic Adjustment and Its Involvement in Adaptation of Tobacco Cells to NaCl. Plant Physiol. 1987 Sep;85(1):174–181. doi: 10.1104/pp.85.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moftah A. E., Michel B. E. The effect of sodium chloride on solute potential and proline accumulation in soybean leaves. Plant Physiol. 1987 Feb;83(2):238–240. doi: 10.1104/pp.83.2.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mortenson E., Dreyfuss G. RNP in maize protein. Nature. 1989 Jan 26;337(6205):312–312. doi: 10.1038/337312b0. [DOI] [PubMed] [Google Scholar]
  19. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  20. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  21. Ohtsuka E., Matsuki S., Ikehara M., Takahashi Y., Matsubara K. An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions. J Biol Chem. 1985 Mar 10;260(5):2605–2608. [PubMed] [Google Scholar]
  22. Singh N. K., Bracker C. A., Hasegawa P. M., Handa A. K., Buckel S., Hermodson M. A., Pfankoch E., Regnier F. E., Bressan R. A. Characterization of osmotin : a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol. 1987 Oct;85(2):529–536. doi: 10.1104/pp.85.2.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Singh N. K., Handa A. K., Hasegawa P. M., Bressan R. A. Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl. Plant Physiol. 1985 Sep;79(1):126–137. doi: 10.1104/pp.79.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Singh N. K., Larosa P. C., Handa A. K., Hasegawa P. M., Bressan R. A. Hormonal regulation of protein synthesis associated with salt tolerance in plant cells. Proc Natl Acad Sci U S A. 1987 Feb;84(3):739–743. doi: 10.1073/pnas.84.3.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stewart C. R., Voetberg G. Relationship between Stress-Induced ABA and Proline Accumulations and ABA-Induced Proline Accumulation in Excised Barley Leaves. Plant Physiol. 1985 Sep;79(1):24–27. doi: 10.1104/pp.79.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Timberlake W. E. Low repetitive DNA content in Aspergillus nidulans. Science. 1978 Dec 1;202(4371):973–975. doi: 10.1126/science.362530. [DOI] [PubMed] [Google Scholar]
  27. Watad A. E., Pesci P. A., Reinhold L., Lerner H. R. Proton Fluxes as a Response to External Salinity in Wild Type and NaCl-Adapted Nicotiana Cell Lines. Plant Physiol. 1986 Jun;81(2):454–459. doi: 10.1104/pp.81.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]

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