Abstract
Tobacco was transformed with a gene coding for an S-locus-specific glycoprotein of Brassica oleracea. The resulting transgenic plants showed tissue-specific and developmentally regulated expression of the introduced gene. Immunolocalization experiments showed that the Brassica gene was expressed in the stylar transmitting tissue of the transgenic plants. The pattern of expression of the introduced gene was more similar to that of the S-associated genes of Nicotiana alata than to expression in Brassica. Self-incompatibility was not conferred by the introduced gene.
Full Text
The Full Text of this article is available as a PDF (3.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson M. A., McFadden G. I., Bernatzky R., Atkinson A., Orpin T., Dedman H., Tregear G., Fernley R., Clarke A. E. Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. Plant Cell. 1989 May;1(5):483–491. doi: 10.1105/tpc.1.5.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
- East E. M., Mangelsdorf A. J. A New Interpretation of the Hereditary Behavior of Self-Sterile Plants. Proc Natl Acad Sci U S A. 1925 Feb;11(2):166–171. doi: 10.1073/pnas.11.2.166. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall T. C., Ma Y., Buchbinder B. U., Pyne J. W., Sun S. M., Bliss F. A. Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3196–3200. doi: 10.1073/pnas.75.7.3196. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kandasamy M. K., Dwyer K. G., Paolillo D. J., Doney R. C., Nasrallah J. B., Nasrallah M. E. Brassica S-Proteins Accumulate in the Intercellular Matrix along the Path of Pollen Tubes in Transgenic Tobacco Pistils. Plant Cell. 1990 Jan;2(1):39–49. doi: 10.1105/tpc.2.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kandasamy M. K., Paolillo D. J., Faraday C. D., Nasrallah J. B., Nasrallah M. E. The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae. Dev Biol. 1989 Aug;134(2):462–472. doi: 10.1016/0012-1606(89)90119-x. [DOI] [PubMed] [Google Scholar]
- Lalonde B. A., Nasrallah M. E., Dwyer K. G., Chen C. H., Barlow B., Nasrallah J. B. A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene. Plant Cell. 1989 Feb;1(2):249–258. doi: 10.1105/tpc.1.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nasrallah J. B., Yu S. M., Nasrallah M. E. Self-incompatibility genes of Brassica oleracea: Expression, isolation, and structure. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5551–5555. doi: 10.1073/pnas.85.15.5551. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothstein S. J., Lahners K. N., Lotstein R. J., Carozzi N. B., Jayne S. M., Rice D. A. Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation. Gene. 1987;53(2-3):153–161. doi: 10.1016/0378-1119(87)90003-5. [DOI] [PubMed] [Google Scholar]
- Sears E R. Cytological Phenomena Connected with Self-Sterility in the Flowering Plants. Genetics. 1937 Jan;22(1):130–181. doi: 10.1093/genetics/22.1.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]