Abstract
A tobacco plant transformed with a Brassica oleracea SLG-22 gene was analyzed by immunocytochemical methods to determine the localization of the transgene-encoded protein product. Immunolabeling was observed in the pistil along the path followed by pollen tubes after pollination. S-antigen accumulated in the intercellular matrix of the transmitting tissue of the style and its continuation in the basal portion of the stigma and outside a few special cells of the placental epidermis of the ovary. This pattern of S-antigen distribution closely resembles that described for the S-associated glycoproteins of self-incompatible Nicotiana alata and differs from its distribution in B. oleracea.
Full Text
The Full Text of this article is available as a PDF (3.3 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson M. A., McFadden G. I., Bernatzky R., Atkinson A., Orpin T., Dedman H., Tregear G., Fernley R., Clarke A. E. Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. Plant Cell. 1989 May;1(5):483–491. doi: 10.1105/tpc.1.5.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barker S. J., Harada J. J., Goldberg R. B. Cellular localization of soybean storage protein mRNA in transformed tobacco seeds. Proc Natl Acad Sci U S A. 1988 Jan;85(2):458–462. doi: 10.1073/pnas.85.2.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bendayan M., Zollinger M. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. J Histochem Cytochem. 1983 Jan;31(1):101–109. doi: 10.1177/31.1.6187796. [DOI] [PubMed] [Google Scholar]
- De Nettancourt D., Devreux M., Bozzini A., Cresti M., Pacini E., Sarfatti G. Ultrastructural aspects of the self-incompatibility mechanism in Lycopersicum peruvianum Mill. J Cell Sci. 1973 Mar;12(2):403–419. doi: 10.1242/jcs.12.2.403. [DOI] [PubMed] [Google Scholar]
- Jefferson R. A., Kavanagh T. A., Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. doi: 10.1002/j.1460-2075.1987.tb02730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kandasamy M. K., Paolillo D. J., Faraday C. D., Nasrallah J. B., Nasrallah M. E. The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae. Dev Biol. 1989 Aug;134(2):462–472. doi: 10.1016/0012-1606(89)90119-x. [DOI] [PubMed] [Google Scholar]
- Moore H. M., Nasrallah J. B. A Brassica Self-Incompatibility Gene Is Expressed in the Stylar Transmitting Tissue of Transgenic Tobacco. Plant Cell. 1990 Jan;2(1):29–38. doi: 10.1105/tpc.2.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nasrallah J. B., Yu S. M., Nasrallah M. E. Self-incompatibility genes of Brassica oleracea: Expression, isolation, and structure. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5551–5555. doi: 10.1073/pnas.85.15.5551. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanders L. C., Lord E. M. Directed movement of latex particles in the gynoecia of three species of flowering plants. Science. 1989 Mar 24;243(4898):1606–1608. doi: 10.1126/science.243.4898.1606. [DOI] [PubMed] [Google Scholar]