Abstract
Visual examination of roots from 12,000 mutagenized Arabidopsis seedlings has led to the identification of more than 40 mutants impaired in root hair morphogenesis. Mutants from four phenotypic classes have been characterized in detail, and genetic tests show that these result from single nuclear recessive mutations in four different genes designated RHD1, RHD2, RHD3, and RHD4. The phenotypic analysis of the mutants and homozygous double mutants has led to a proposed model for root hair development and the stages at which the genes are normally required. The RHD1 gene product appears to be necessary for proper initiation of root hairs, whereas the RHD2, RHD3, and RHD4 gene products are required for normal hair elongation. These results demonstrate that root hair development in Arabidopsis is amenable to genetic dissection and should prove to be a useful model system to study the molecular mechanisms governing cell differentiation in plants.
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Selected References
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- Caspar T., Pickard B. G. Gravitropism in a starchless mutant of Arabidopsis: implications for the starch-statolith theory of gravity sensing. Planta. 1989;177:185–197. [PubMed] [Google Scholar]
- Chang C., Bowman J. L., DeJohn A. W., Lander E. S., Meyerowitz E. M. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856–6860. doi: 10.1073/pnas.85.18.6856. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marks M. D., Feldmann K. A. Trichome Development in Arabidopsis thaliana. I. T-DNA Tagging of the GLABROUS1 Gene. Plant Cell. 1989 Nov;1(11):1043–1050. doi: 10.1105/tpc.1.11.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nam H. G., Giraudat J., Den Boer B., Moonan F., Loos WDB., Hauge B. M., Goodman H. M. Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana. Plant Cell. 1989 Jul;1(7):699–705. doi: 10.1105/tpc.1.7.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newcomb W., Wood S. M. Morphogenesis and fine structure of Frankia (Actinomycetales): the microsymbiont of nitrogen-fixing actinorhizal root nodules. Int Rev Cytol. 1987;109:1–88. doi: 10.1016/s0074-7696(08)61719-2. [DOI] [PubMed] [Google Scholar]