Skip to main content
NCBI home page
The Plant Cell logoLink to The Plant Cell
. 1990 May;2(5):427–436. doi: 10.1105/tpc.2.5.427

Differential expression of five Arabidopsis genes encoding glycine-rich proteins.

D E de Oliveira 1, J Seurinck 1, D Inzé 1, M Van Montagu 1, J Botterman 1
PMCID: PMC159899  PMID: 2152168

Abstract

Five cDNA clones coding for glycine-rich proteins in Arabidopsis thaliana were isolated. The corresponding genes are present in the genome as single copies. The derived protein sequences contain highly repetitive glycine-rich motifs. There is, however, little homology among them, nor with previously described glycine-rich proteins from other species. All five genes are expressed in leaves and stems of 6-week-old plants but show different patterns of expression in other organ systems. Analysis of the effect of different external stimuli on the expression pattern showed that, in most cases, the transcript levels were moderately but selectively affected. With flooding stress, the accumulated level of the transcript from one of the genes was remarkably increased.

Full Text

The Full Text of this article is available as a PDF (2.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alliotte T., Tiré C., Engler G., Peleman J., Caplan A., Van Montagu M., Inzé D. An Auxin-Regulated Gene of Arabidopsis thaliana Encodes a DNA-Binding Protein. Plant Physiol. 1989 Mar;89(3):743–752. doi: 10.1104/pp.89.3.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen J., Varner J. E. An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin. EMBO J. 1985 Sep;4(9):2145–2151. doi: 10.1002/j.1460-2075.1985.tb03908.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Corbin D. R., Sauer N., Lamb C. J. Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants. Mol Cell Biol. 1987 Dec;7(12):4337–4344. doi: 10.1128/mcb.7.12.4337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Datta K., Schmidt A., Marcus A. Characterization of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes. Plant Cell. 1989 Sep;1(9):945–952. doi: 10.1105/tpc.1.9.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gómez J., Sánchez-Martínez D., Stiefel V., Rigau J., Puigdomènech P., Pagès M. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature. 1988 Jul 21;334(6179):262–264. doi: 10.1038/334262a0. [DOI] [PubMed] [Google Scholar]
  6. Jones J. D., Dunsmuir P., Bedbrook J. High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 1985 Oct;4(10):2411–2418. doi: 10.1002/j.1460-2075.1985.tb03949.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaldenhoff R., Richter G. Sequence of cDNA for a novel light-induced glycine-rich protein. Nucleic Acids Res. 1989 Apr 11;17(7):2853–2853. doi: 10.1093/nar/17.7.2853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keller B., Sauer N., Lamb C. J. Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. EMBO J. 1988 Dec 1;7(12):3625–3633. doi: 10.1002/j.1460-2075.1988.tb03243.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keller B., Schmid J., Lamb C. J. Vascular expression of a bean cell wall glycine-rich protein-beta-glucuronidase gene fusion in transgenic tobacco. EMBO J. 1989 May;8(5):1309–1314. doi: 10.1002/j.1460-2075.1989.tb03510.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lawton M. A., Lamb C. J. Transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection. Mol Cell Biol. 1987 Jan;7(1):335–341. doi: 10.1128/mcb.7.1.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Linthorst H. J., Meuwissen R. L., Kauffmann S., Bol J. F. Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection. Plant Cell. 1989 Mar;1(3):285–291. doi: 10.1105/tpc.1.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pruitt R. E., Meyerowitz E. M. Characterization of the genome of Arabidopsis thaliana. J Mol Biol. 1986 Jan 20;187(2):169–183. doi: 10.1016/0022-2836(86)90226-3. [DOI] [PubMed] [Google Scholar]
  14. Reddy A. S., Poovaiah B. W. Accumulation of a glycine rich protein in auxin-deprived strawberry fruits. Biochem Biophys Res Commun. 1987 Sep 30;147(3):885–891. doi: 10.1016/s0006-291x(87)80153-5. [DOI] [PubMed] [Google Scholar]
  15. Showalter A. M., Bell J. N., Cramer C. L., Bailey J. A., Varner J. E., Lamb C. J. Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6551–6555. doi: 10.1073/pnas.82.19.6551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Simoens C. R., Peleman J., Valvekens D., Van Montagu M., Inzé D. Isolation of genes expressed in specific tissues of Arabidopsis thaliana by differential screening of a genomic library. Gene. 1988 Jul 15;67(1):1–11. doi: 10.1016/0378-1119(88)90002-9. [DOI] [PubMed] [Google Scholar]
  17. Thomas E. D. Bone marrow grafts and tolerance. Nature. 1986 Sep 11;323(6084):110–111. doi: 10.1038/323110b0. [DOI] [PubMed] [Google Scholar]
  18. Verwoerd T. C., Dekker B. M., Hoekema A. A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 1989 Mar 25;17(6):2362–2362. doi: 10.1093/nar/17.6.2362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Kan J. A., Cornelissen B. J., Bol J. F. A virus-inducible tobacco gene encoding a glycine-rich protein shares putative regulatory elements with the ribulose bisphosphate carboxylase small subunit gene. Mol Plant Microbe Interact. 1988 Mar;1(3):107–112. doi: 10.1094/mpmi-1-107. [DOI] [PubMed] [Google Scholar]
  20. von Heijne G. Transcending the impenetrable: how proteins come to terms with membranes. Biochim Biophys Acta. 1988 Jun 9;947(2):307–333. doi: 10.1016/0304-4157(88)90013-5. [DOI] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES