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. 1990 Jun;2(6):569–579. doi: 10.1105/tpc.2.6.569

Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid.

H S Mason 1, J E Mullet 1
PMCID: PMC159912  PMID: 2152178

Abstract

The expression of vspA and vspB genes encoding soybean vegetative storage proteins was studied during seedling development and in response to water deficit, tissue wounding, and jasmonic acid treatment. vspA and vspB encode VSP-alpha and VSP-beta, 28-kilodalton and 31-kilodalton vacuole-localized polypeptides that are 80% homologous. vspA and vspB mRNAs could be distinguished on RNA blots using 3'-end probes. vspA mRNA was threefold to sevenfold more abundant than vspB mRNA in leaves, about equal expression was observed in stems, and vspB mRNA exceeded vspA in roots. Transcripts were not detected in dry seeds but appeared in intact or excised seedling axes between 12 hr and 24 hr after initiation of imbibition. Both transcripts were highly abundant in the meristematic region of seedling stems and in developing leaves but were rare in mature stems, leaves, and roots. In situ localization showed that vsp transcripts were found throughout the hypocotyl hook but were concentrated in cells associated with the epidermis and vascular bundles. Water deficit caused increased vsp mRNA levels in leaves and stems, which suggests that inhibition of growth necessitates temporary storage of amino acids. Wounding induced primarily vspB mRNA in etiolated seedlings, whereas both vspA and vspB mRNA levels increased in wounded leaves. Jasmonic acid and methyl jasmonate were potent inducers of vsp gene expression in cell cultures, developing axes, leaves, and roots. We hypothesize that jasmonic acid levels modulate vsp mRNA abundance in vivo.

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Selected References

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  1. Andrews D. L., Beames B., Summers M. D., Park W. D. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector. Biochem J. 1988 May 15;252(1):199–206. doi: 10.1042/bj2520199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bozarth C. S., Mullet J. E., Boyer J. S. Cell wall proteins at low water potentials. Plant Physiol. 1987 Sep;85(1):261–267. doi: 10.1104/pp.85.1.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Creelman R. A., Mason H. S., Bensen R. J., Boyer J. S., Mullet J. E. Water Deficit and Abscisic Acid Cause Differential Inhibition of Shoot versus Root Growth in Soybean Seedlings : Analysis of Growth, Sugar Accumulation, and Gene Expression. Plant Physiol. 1990 Jan;92(1):205–214. doi: 10.1104/pp.92.1.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dobres M. S., Thompson W. F. A developmentally regulated bud specific transcript in pea has sequence similarity to seed lectins. Plant Physiol. 1989 Mar;89(3):833–838. doi: 10.1104/pp.89.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Franceschi V. R., Wittenbach V. A., Giaquinta R. T. Paraveinal Mesophyll of Soybean Leaves in Relation to Assimilate Transfer and Compartmentation : III. Immunohistochemical Localization of Specific Glycopeptides in the Vacuole after Depodding. Plant Physiol. 1983 Jun;72(2):586–589. doi: 10.1104/pp.72.2.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herman E. M., Hankins C. N., Shannon L. M. Bark and Leaf Lectins of Sophora japonica Are Sequestered in Protein-Storage Vacuoles. Plant Physiol. 1988 Apr;86(4):1027–1031. doi: 10.1104/pp.86.4.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Horn M. E., Sherrard J. H., Widholm J. M. Photoautotrophic growth of soybean cells in suspension culture: I. Establishment of photoautotrophic cultures. Plant Physiol. 1983 Jun;72(2):426–429. doi: 10.1104/pp.72.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Spilatro S. R., Anderson J. M. Characterization of a soybean leaf protein that is related to the seed lectin and is increased with pod removal. Plant Physiol. 1989 Aug;90(4):1387–1393. doi: 10.1104/pp.90.4.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Staswick P. E. Developmental regulation and the influence of plant sinks on vegetative storage protein gene expression in soybean leaves. Plant Physiol. 1989 Jan;89(1):309–315. doi: 10.1104/pp.89.1.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Staswick P. E. Novel Regulation of Vegetative Storage Protein Genes. Plant Cell. 1990 Jan;2(1):1–6. doi: 10.1105/tpc.2.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Staswick P. E. Preferential Loss of an Abundant Storage Protein from Soybean Pods during Seed Development. Plant Physiol. 1989 Aug;90(4):1252–1255. doi: 10.1104/pp.90.4.1252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Staswick P. E. Soybean vegetative storage protein structure and gene expression. Plant Physiol. 1988 May;87(1):250–254. doi: 10.1104/pp.87.1.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wittenbach V. A. Effect of pod removal on leaf senescence in soybeans. Plant Physiol. 1982 Nov;70(5):1544–1548. doi: 10.1104/pp.70.5.1544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wittenbach V. A. Purification and characterization of a soybean leaf storage glycoprotein. Plant Physiol. 1983 Sep;73(1):125–129. doi: 10.1104/pp.73.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]

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