Abstract
A set of cDNA clones have been characterized that represent early nodulin mRNAs from pea root nodules. By RNA transfer blot analyses, the different early nodulin mRNAs were found to vary in time course of appearance during the development of the indeterminate pea root nodule. In situ hybridization studies demonstrated that the transcripts were located in different zones, representing subsequent steps in development of the central tissue of the root nodule. ENOD12 transcripts were present in every cell of the invasion zone, whereas ENOD5, ENOD3, and ENOD14 transcripts were restricted to the infected cells in successive but partially overlapping zones of the central tissue. We conclude that the corresponding nodulin genes are expressed at subsequent developmental stages. The amino acid sequence derived from the nucleotide sequences of the cDNAs, in combination with the localization data, showed that ENOD5 is an arabinogalactan-like protein involved in the infection process, whereas ENOD3 and ENOD14 have a cysteine cluster suggesting that these are metal-binding proteins. Furthermore, we showed that there is a clear difference in the way Rhizobium induced the infection-related early nodulin genes ENOD5 and ENOD12. A factor acting over a long distance induced the ENOD12 gene, whereas a factor acting over a short distance activated the ENOD5 gene.
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Selected References
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- Berg J. M. Potential metal-binding domains in nucleic acid binding proteins. Science. 1986 Apr 25;232(4749):485–487. doi: 10.1126/science.2421409. [DOI] [PubMed] [Google Scholar]
- Bisseling T., van den Bos R. C., van Kammen A. The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum. Biochim Biophys Acta. 1978 Feb 13;539(1):1–11. doi: 10.1016/0304-4165(78)90115-0. [DOI] [PubMed] [Google Scholar]
- Franssen H. J., Nap J. P., Gloudemans T., Stiekema W., Van Dam H., Govers F., Louwerse J., Van Kammen A., Bisseling T. Characterization of cDNA for nodulin-75 of soybean: A gene product involved in early stages of root nodule development. Proc Natl Acad Sci U S A. 1987 Jul;84(13):4495–4499. doi: 10.1073/pnas.84.13.4495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Govers F., Gloudemans T., Moerman M., van Kammen A., Bisseling T. Expression of plant genes during the development of pea root nodules. EMBO J. 1985 Apr;4(4):861–867. doi: 10.1002/j.1460-2075.1985.tb03711.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobs F. A., Zhang M., Fortin M. G., Verma D. P. Several nodulins of soybean share structural domains but differ in their subcellular locations. Nucleic Acids Res. 1987 Feb 11;15(3):1271–1280. doi: 10.1093/nar/15.3.1271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987 Jan;6(1):43–48. doi: 10.1002/j.1460-2075.1987.tb04716.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigaud G., Grange T., Pictet R. The use of NaOH as transfer solution of DNA onto nylon membrane decreases the hybridization efficiency. Nucleic Acids Res. 1987 Jan 26;15(2):857–857. doi: 10.1093/nar/15.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheres B., Van De Wiel C., Zalensky A., Horvath B., Spaink H., Van Eck H., Zwartkruis F., Wolters A. M., Gloudemans T., Van Kammen A. The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction. Cell. 1990 Jan 26;60(2):281–294. doi: 10.1016/0092-8674(90)90743-x. [DOI] [PubMed] [Google Scholar]
- van Holst G. J., Klis F. M., de Wildt P. J., Hazenberg C. A., Buijs J., Stegwee D. Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus vulgaris L. Plant Physiol. 1981 Oct;68(4):910–913. doi: 10.1104/pp.68.4.910. [DOI] [PMC free article] [PubMed] [Google Scholar]