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. 1990 Sep;2(9):867–876. doi: 10.1105/tpc.2.9.867

Cloning of the y1 Locus of Maize, a Gene Involved in the Biosynthesis of Carotenoids.

B Buckner 1, TL Kelson 1, DS Robertson 1
PMCID: PMC159937  PMID: 12354969

Abstract

The y1 gene is one of the genes responsible for the production of [beta]-carotene in the endosperm and leaves of maize. We have cloned a Robertson's Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolate the standard Y1 allele, which conditions the presence of [beta]-carotene in the endosperm of the maize kernel.

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Selected References

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