Skip to main content
PMC home page
The Plant Cell logoLink to The Plant Cell
. 1990 Sep;2(9):913–924. doi: 10.1105/tpc.2.9.913

Cytochrome c-553 is not required for photosynthetic activity in the cyanobacterium Synechococcus.

D E Laudenbach 1, S K Herbert 1, C McDowell 1, D C Fork 1, A R Grossman 1, N A Straus 1
PMCID: PMC159941  PMID: 1967057

Abstract

In cyanobacteria, the water-soluble cytochrome c-553 functions as a mobile carrier of electrons between the membrane-bound cytochrome b6-f complex and P-700 reaction centers of Photosystem I. The structural gene for cytochrome c-553 (designated cytA) of the cyanobacterium Synechococcus sp. PCC 7942 was cloned, and the deduced amino acid sequence was shown to be similar to known cyanobacterial cytochrome c-553 proteins. A deletion mutant was constructed that had no detectable cytochrome c-553 based on spectral analyses and tetramethylbenzidine-hydrogen peroxide staining of proteins resolved by polyacrylamide gel electrophoresis. The mutant strain was not impaired in overall photosynthetic activity. However, this mutant exhibited a decreased efficiency of cytochrome f oxidation. These results indicate that cytochrome c-553 is not an absolute requirement for reducing Photosystem I reaction centers in Synechococcus sp. PCC 7942.

Full Text

The Full Text of this article is available as a PDF (1.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Block M. A., Grossman A. R. Identification and Purification of a Derepressible Alkaline Phosphatase from Anacystis nidulans R2. Plant Physiol. 1988 Apr;86(4):1179–1184. doi: 10.1104/pp.86.4.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis D. J., Krogmann D. W., Pietro A. S. Electron donation to photosystem I. Plant Physiol. 1980 Apr;65(4):697–702. doi: 10.1104/pp.65.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  4. Green L. S., Grossman A. R. Changes in sulfate transport characteristics and protein composition of Anacystis nidulans R2 during sulfur deprivation. J Bacteriol. 1988 Feb;170(2):583–587. doi: 10.1128/jb.170.2.583-587.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kiley P. J., Kaplan S. Molecular genetics of photosynthetic membrane biosynthesis in Rhodobacter sphaeroides. Microbiol Rev. 1988 Mar;52(1):50–69. doi: 10.1128/mr.52.1.50-69.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Merchant S., Bogorad L. Rapid degradation of apoplastocyanin in Cu(II)-deficient cells of Chlamydomonas reinhardtii. J Biol Chem. 1986 Dec 5;261(34):15850–15853. [PubMed] [Google Scholar]
  7. Merchant S., Bogorad L. Regulation by copper of the expression of plastocyanin and cytochrome c552 in Chlamydomonas reinhardi. Mol Cell Biol. 1986 Feb;6(2):462–469. doi: 10.1128/mcb.6.2.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murray M. G. Use of sodium trichloroacetate and mung bean nuclease to increase sensitivity and precision during transcript mapping. Anal Biochem. 1986 Oct;158(1):165–170. doi: 10.1016/0003-2697(86)90605-6. [DOI] [PubMed] [Google Scholar]
  9. Nierzwicki-Bauer S. A., Balkwill D. L., Stevens S. E., Jr Three-dimensional ultrastructure of a unicellular cyanobacterium. J Cell Biol. 1983 Sep;97(3):713–722. doi: 10.1083/jcb.97.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Peschek G. A., Schmetterer G. Evidence for plastoquinol-cytochrome f/b-563 reductase as a common electron donor to P700 and cytochrome oxidase in cyanobacteria. Biochem Biophys Res Commun. 1982 Oct 15;108(3):1188–1195. doi: 10.1016/0006-291x(82)92126-x. [DOI] [PubMed] [Google Scholar]
  11. Prentki P., Krisch H. M. In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984 Sep;29(3):303–313. doi: 10.1016/0378-1119(84)90059-3. [DOI] [PubMed] [Google Scholar]
  12. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stanier R. Y., Cohen-Bazire G. Phototrophic prokaryotes: the cyanobacteria. Annu Rev Microbiol. 1977;31:225–274. doi: 10.1146/annurev.mi.31.100177.001301. [DOI] [PubMed] [Google Scholar]
  15. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tandeau de Marsac N., Borrias W. E., Kuhlemeier C. J., Castets A. M., van Arkel G. A., van den Hondel C. A. A new approach for molecular cloning in cyanobacteria: cloning of an Anacystis nidulans met gene using a Tn901-induced mutant. Gene. 1982 Nov;20(1):111–119. doi: 10.1016/0378-1119(82)90092-0. [DOI] [PubMed] [Google Scholar]
  17. Thomas P. E., Ryan D., Levin W. An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels. Anal Biochem. 1976 Sep;75(1):168–176. doi: 10.1016/0003-2697(76)90067-1. [DOI] [PubMed] [Google Scholar]
  18. Tomioka N., Sugiura M. The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans. Mol Gen Genet. 1983;191(1):46–50. doi: 10.1007/BF00330888. [DOI] [PubMed] [Google Scholar]
  19. Ulrich E. L., Krogmann D. W., Markley J. L. Structure and heme environment of ferrocytochrome c553 from 1H NMR studies. J Biol Chem. 1982 Aug 25;257(16):9356–9364. [PubMed] [Google Scholar]
  20. Van der Plas J., Bovy A., Kruyt F., de Vrieze G., Dassen E., Klein B., Weisbeek P. The gene for the precursor of plastocyanin from the cyanobacterium Anabaena sp. PCC 7937: isolation, sequence and regulation. Mol Microbiol. 1989 Mar;3(3):275–284. doi: 10.1111/j.1365-2958.1989.tb00172.x. [DOI] [PubMed] [Google Scholar]
  21. Wood P. M. Interchangeable copper and iron proteins in algal photosynthesis. Studies on plastocyanin and cytochrome c-552 in Chlamydomonas. Eur J Biochem. 1978 Jun 1;87(1):9–19. doi: 10.1111/j.1432-1033.1978.tb12346.x. [DOI] [PubMed] [Google Scholar]
  22. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES