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. 1991 May;3(5):541–550. doi: 10.1105/tpc.3.5.541

Circadian Control of cab Gene Transcription and mRNA Accumulation in Arabidopsis.

AJ Millar 1, SA Kay 1
PMCID: PMC160021  PMID: 12324603

Abstract

An intriguing property of many organisms is their ability to exhibit rhythmic cellular events that continue independently of environmental stimuli. These rhythmic processes are generated by an endogenous mechanism known as the biological clock. We wished to determine whether Arabidopsis thaliana will serve as a model plant system for a molecular genetic dissection of the circadian clock. To this end, we investigated the expression of Arabidopsis chlorophyll a/b-binding protein (cab) genes throughout the circadian cycle. Steady-state mRNA levels of the cab2 and cab3 genes showed a dramatic circadian cycling in plants shifted from light/dark cycles to constant darkness, whereas the cab1 mRNA level exhibited little or no cycling under the same conditions. Analysis of cab promoter fusions in transgenic tobacco revealed that both the cab1 and cab2 5[prime] upstream regions confer circadian-regulated expression on a chloramphenicol acetyltransferase (cat) reporter gene. In vitro nuclear run-on transcription assays also indicated that the transcription of the cab1 and cab2 genes is circadian regulated in Arabidopsis. Taken together, these data suggest that a post-transcriptional mechanism influences cab1 mRNA levels in Arabidopsis. The identification of circadian-regulated cis-acting elements in the cab1 and cab2 upstream regions will provide powerful tools for both molecular and genetic analysis of the higher plant circadian clock.

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Selected References

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  1. Boutry M., Chua N. H. A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. EMBO J. 1985 Sep;4(9):2159–2165. doi: 10.1002/j.1460-2075.1985.tb03910.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dean C., Favreau M., Bond-Nutter D., Bedbrook J., Dunsmuir P. Sequences downstream of translation start regulate quantitative expression of two petunia rbcS genes. Plant Cell. 1989 Feb;1(2):201–208. doi: 10.1105/tpc.1.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dunlap J. C. Closely watched clocks: molecular analysis of circadian rhythms in Neurospora and Drosophila. Trends Genet. 1990 May;6(5):159–165. doi: 10.1016/0168-9525(90)90151-u. [DOI] [PubMed] [Google Scholar]
  4. Fejes E., Pay A., Kanevsky I., Szell M., Adam E., Kay S., Nagy F. A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants. Plant Mol Biol. 1990 Dec;15(6):921–932. doi: 10.1007/BF00039431. [DOI] [PubMed] [Google Scholar]
  5. Giuliano G., Hoffman N. E., Ko K., Scolnik P. A., Cashmore A. R. A light-entrained circadian clock controls transcription of several plant genes. EMBO J. 1988 Dec 1;7(12):3635–3642. doi: 10.1002/j.1460-2075.1988.tb03244.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hardin P. E., Hall J. C., Rosbash M. Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels. Nature. 1990 Feb 8;343(6258):536–540. doi: 10.1038/343536a0. [DOI] [PubMed] [Google Scholar]
  7. Huang T. C., Tu J., Chow T. J., Chen T. H. Circadian Rhythm of the Prokaryote Synechococcus sp. RF-1. Plant Physiol. 1990 Feb;92(2):531–533. doi: 10.1104/pp.92.2.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ingelbrecht I. L., Herman L. M., Dekeyser R. A., Van Montagu M. C., Depicker A. G. Different 3' end regions strongly influence the level of gene expression in plant cells. Plant Cell. 1989 Jul;1(7):671–680. doi: 10.1105/tpc.1.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jefferson R. A., Kavanagh T. A., Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. doi: 10.1002/j.1460-2075.1987.tb02730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karlin-Neumann G. A., Sun L., Tobin E. M. Expression of Light-Harvesting Chlorophyll a/b-Protein Genes Is Phytochrome-Regulated in Etiolated Arabidopsis thaliana Seedlings. Plant Physiol. 1988 Dec;88(4):1323–1331. doi: 10.1104/pp.88.4.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leutwiler L. S., Meyerowitz E. M., Tobin E. M. Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana. Nucleic Acids Res. 1986 May 27;14(10):4051–4064. doi: 10.1093/nar/14.10.4051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lissemore J. L., Quail P. H. Rapid transcriptional regulation by phytochrome of the genes for phytochrome and chlorophyll a/b-binding protein in Avena sativa. Mol Cell Biol. 1988 Nov;8(11):4840–4850. doi: 10.1128/mcb.8.11.4840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Loros J. J., Dunlap J. C. Neurospora crassa clock-controlled genes are regulated at the level of transcription. Mol Cell Biol. 1991 Jan;11(1):558–563. doi: 10.1128/mcb.11.1.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Paulsen H., Bogorad L. Diurnal and Circadian Rhythms in the Accumulation and Synthesis of mRNA for the Light-Harvesting Chlorophyll a/b-Binding Protein in Tobacco. Plant Physiol. 1988 Dec;88(4):1104–1109. doi: 10.1104/pp.88.4.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosbash M., Hall J. C. The molecular biology of circadian rhythms. Neuron. 1989 Oct;3(4):387–398. doi: 10.1016/0896-6273(89)90199-2. [DOI] [PubMed] [Google Scholar]
  16. Schneider M., Ow D. W., Howell S. H. The in vivo pattern of firefly luciferase expression in transgenic plants. Plant Mol Biol. 1990 Jun;14(6):935–947. doi: 10.1007/BF00019391. [DOI] [PubMed] [Google Scholar]
  17. Silverthorne J., Tobin E. M. Post-transcriptional regulation of organ-specific expression of individual rbcS mRNAs in Lemna gibba. Plant Cell. 1990 Dec;2(12):1181–1190. doi: 10.1105/tpc.2.12.1181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simon E., Satter R. L., Galston A. W. Circadian Rhythmicity in Excised Samanea Pulvini: II. Resetting the Clock by Phytochrome Conversion. Plant Physiol. 1976 Sep;58(3):421–425. doi: 10.1104/pp.58.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stayton M. M., Brosio P., Dunsmuir P. Photosynthetic Genes of Petunia (Mitchell) Are Differentially Expressed during the Diurnal Cycle. Plant Physiol. 1989 Mar;89(3):776–782. doi: 10.1104/pp.89.3.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor W. C. Transcriptional regulation by a circadian rhythm. Plant Cell. 1989 Feb;1(2):259–264. doi: 10.1105/tpc.1.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Uhl G. R., Reppert S. M. Suprachiasmatic nucleus vasopressin messenger RNA: circadian variation in normal and Brattleboro rats. Science. 1986 Apr 18;232(4748):390–393. doi: 10.1126/science.3961487. [DOI] [PubMed] [Google Scholar]
  22. Walling L., Drews G. N., Goldberg R. B. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123–2127. doi: 10.1073/pnas.83.7.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wuarin J., Schibler U. Expression of the liver-enriched transcriptional activator protein DBP follows a stringent circadian rhythm. Cell. 1990 Dec 21;63(6):1257–1266. doi: 10.1016/0092-8674(90)90421-a. [DOI] [PubMed] [Google Scholar]
  24. Zerr D. M., Hall J. C., Rosbash M., Siwicki K. K. Circadian fluctuations of period protein immunoreactivity in the CNS and the visual system of Drosophila. J Neurosci. 1990 Aug;10(8):2749–2762. doi: 10.1523/JNEUROSCI.10-08-02749.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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