Abstract
Fungi typically grow by apical extension of hyphae that penetrate moist substrates. After establishing a branched feeding mycelium, the hyphae differentiate and grow away from the substrate into the air where they form various structures such as aerial hyphae and mushrooms. In the basidiomycete species Schizophyllum commune, we previously identified a family of homologous genes that code for small cysteine-rich hydrophobic proteins. We now report that the encoded hydrophobins are excreted in abundance into the culture medium by submerged feeding hyphae but form highly insoluble complexes in the walls of emerging hyphae. The Sc3 gene encodes a hydrophobin present in walls of aerial hyphae. The homologous Sc1 and Sc4 genes, which are regulated by the mating-type genes, encode hydrophobins present in walls of fruit body hyphae. The hydrophobins are probably instrumental in the emergence of these aerial structures.
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Selected References
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- Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
- Dons J. J., Mulder G. H., Rouwendal G. J., Springer J., Bremer W., Wessels J. G. Sequence analysis of a split gene involved in fruiting from the fungus Schizophyllum commune. EMBO J. 1984 Sep;3(9):2101–2106. doi: 10.1002/j.1460-2075.1984.tb02097.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dons J. J., de Vries O. M., Wessels J. G. Characterization of the genome of the basidiomycete Schizophyllum commune. Biochim Biophys Acta. 1979 Jun 20;563(1):100–112. doi: 10.1016/0005-2787(79)90011-x. [DOI] [PubMed] [Google Scholar]
- Jovin T. M. Multiphasic zone electrophoresis. 3. Further analysis and new forms of discontinuous buffer systems. Biochemistry. 1973 Feb 27;12(5):890–898. doi: 10.1021/bi00729a016. [DOI] [PubMed] [Google Scholar]
- Schuren F. H., Wessels J. G. Two genes specifically expressed in fruiting dikaryons of Schizophyllum commune: homologies with a gene not regulated by mating-type genes. Gene. 1990 Jun 15;90(2):199–205. doi: 10.1016/0378-1119(90)90180-y. [DOI] [PubMed] [Google Scholar]