Abstract
Because numerous attempts to detect an activity for a cellulose synthase in plants have failed, we have taken a different approach toward detecting polypeptides involved in this process. The uniqueness of the structure and function of cyclic diguanylic acid (c-di-GMP) as an activator of the cellulose synthase of the bacterium Acetobacter xylinum makes it an attractive probe to use in a search for a c-di-GMP receptor that might be involved in the process in plants. Direct photolabeling with 32P-c-di-GMP has been used, therefore, to identify in plants two membrane polypeptides of 83 and 48 kD derived from cotton fibers that possess properties consistent with their being components of a c-di-GMP-dependent cellulose synthase. Based upon several criteria, the 48-kD species is proposed to be derived by proteolytic cleavage of the 83-kD polypeptide. Both polypeptides bind c-di-GMP with high affinity and specificity and show antigenic relatedness to the bacterial cellulose synthase, and the N-terminal sequence of the 48-kD polypeptide also indicates relatedness to the bacterial synthase. Ability to detect both cotton fiber polypeptides by photolabeling increases markedly in extracts derived from fibers entering the active phase of secondary wall cellulose synthesis. These results provide a basis for future work aimed at identifying and characterizing genes involved in cellulose synthesis in plants.
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Selected References
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- Delmer D. P., Solomon M., Read S. M. Direct Photolabeling with [P]UDP-Glucose for Identification of a Subunit of Cotton Fiber Callose Synthase. Plant Physiol. 1991 Feb;95(2):556–563. doi: 10.1104/pp.95.2.556. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elthon T. E., McIntosh L. Identification of the alternative terminal oxidase of higher plant mitochondria. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8399–8403. doi: 10.1073/pnas.84.23.8399. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed] [Google Scholar]
- Mayer R., Ross P., Weinhouse H., Amikam D., Volman G., Ohana P., Calhoon R. D., Wong H. C., Emerick A. W., Benziman M. Polypeptide composition of bacterial cyclic diguanylic acid-dependent cellulose synthase and the occurrence of immunologically crossreacting proteins in higher plants. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5472–5476. doi: 10.1073/pnas.88.12.5472. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meinert M. C., Delmer D. P. Changes in biochemical composition of the cell wall of the cotton fiber during development. Plant Physiol. 1977 Jun;59(6):1088–1097. doi: 10.1104/pp.59.6.1088. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ross P., Mayer R., Benziman M. Cellulose biosynthesis and function in bacteria. Microbiol Rev. 1991 Mar;55(1):35–58. doi: 10.1128/mr.55.1.35-58.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ross P., Mayer R., Weinhouse H., Amikam D., Huggirat Y., Benziman M., de Vroom E., Fidder A., de Paus P., Sliedregt L. A. The cyclic diguanylic acid regulatory system of cellulose synthesis in Acetobacter xylinum. Chemical synthesis and biological activity of cyclic nucleotide dimer, trimer, and phosphothioate derivatives. J Biol Chem. 1990 Nov 5;265(31):18933–18943. [PubMed] [Google Scholar]
- Saxena I. M., Lin F. C., Brown R. M., Jr Cloning and sequencing of the cellulose synthase catalytic subunit gene of Acetobacter xylinum. Plant Mol Biol. 1990 Nov;15(5):673–683. doi: 10.1007/BF00016118. [DOI] [PubMed] [Google Scholar]
- Saxena I. M., Lin F. C., Brown R. M., Jr Identification of a new gene in an operon for cellulose biosynthesis in Acetobacter xylinum. Plant Mol Biol. 1991 Jun;16(6):947–954. doi: 10.1007/BF00016067. [DOI] [PubMed] [Google Scholar]
- Wong H. C., Fear A. L., Calhoon R. D., Eichinger G. H., Mayer R., Amikam D., Benziman M., Gelfand D. H., Meade J. H., Emerick A. W. Genetic organization of the cellulose synthase operon in Acetobacter xylinum. Proc Natl Acad Sci U S A. 1990 Oct;87(20):8130–8134. doi: 10.1073/pnas.87.20.8130. [DOI] [PMC free article] [PubMed] [Google Scholar]