Skip to main content
PMC home page
The Plant Cell logoLink to The Plant Cell
. 1992 Jan;4(1):29–38. doi: 10.1105/tpc.4.1.29

Red Light-Independent Instability of Oat Phytochrome mRNA in Vivo.

KA Seeley 1, DH Byrne 1, JT Colbert 1
PMCID: PMC160103  PMID: 12297628

Abstract

Phytochrome A (phyA) mRNA abundance decreased rapidly in total RNA samples isolated from 4-day-old etiolated oat seedlings following a red light pulse. Putative in vivo phyA mRNA degradation products were detectable both before and after red light treatment. Cordycepin-treated coleoptiles were unable to accumulate the chlorophyll a/b-binding protein mRNA in response to red light, indicating that cordycepin effectively inhibited mRNA synthesis. In cordycepin-treated coleoptiles, phyA mRNA rapidly decreased in abundance, consistent with the hypothesis that phyA mRNA is inherently unstable, rather than being destabilized after red light treatment of etiolated oat seedlings.

Full Text

The Full Text of this article is available as a PDF (3.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atwater J. A., Wisdom R., Verma I. M. Regulated mRNA stability. Annu Rev Genet. 1990;24:519–541. doi: 10.1146/annurev.ge.24.120190.002511. [DOI] [PubMed] [Google Scholar]
  2. Boylan M. T., Quail P. H. Oat Phytochrome Is Biologically Active in Transgenic Tomatoes. Plant Cell. 1989 Aug;1(8):765–773. doi: 10.1105/tpc.1.8.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brewer G., Ross J. Regulation of c-myc mRNA stability in vitro by a labile destabilizer with an essential nucleic acid component. Mol Cell Biol. 1989 May;9(5):1996–2006. doi: 10.1128/mcb.9.5.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brock M. L., Shapiro D. J. Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation. Cell. 1983 Aug;34(1):207–214. doi: 10.1016/0092-8674(83)90151-4. [DOI] [PubMed] [Google Scholar]
  5. Casey J. L., Hentze M. W., Koeller D. M., Caughman S. W., Rouault T. A., Klausner R. D., Harford J. B. Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation. Science. 1988 May 13;240(4854):924–928. doi: 10.1126/science.2452485. [DOI] [PubMed] [Google Scholar]
  6. Cline M. G., Rehm M. M. Rapid Inhibition of Auxin-induced Elongation of Avena Coleoptile Segments by Cordycepin. Plant Physiol. 1974 Aug;54(2):160–163. doi: 10.1104/pp.54.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Colbert J. T., Costigan S. A., Zhao Z. Photoregulation of beta-Tubulin mRNA Abundance in Etiolated Oat and Barley Seedlings. Plant Physiol. 1990 Jul;93(3):1196–1202. doi: 10.1104/pp.93.3.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Colbert J. T., Hershey H. P., Quail P. H. Autoregulatory control of translatable phytochrome mRNA levels. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2248–2252. doi: 10.1073/pnas.80.8.2248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dean C., Elzen P., Tamaki S., Dunsmuir P., Bedbrook J. Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multi-gene family. EMBO J. 1985 Dec 1;4(12):3055–3061. doi: 10.1002/j.1460-2075.1985.tb04045.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gay D. A., Sisodia S. S., Cleveland D. W. Autoregulatory control of beta-tubulin mRNA stability is linked to translation elongation. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5763–5767. doi: 10.1073/pnas.86.15.5763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Graves R. A., Pandey N. B., Chodchoy N., Marzluff W. F. Translation is required for regulation of histone mRNA degradation. Cell. 1987 Feb 27;48(4):615–626. doi: 10.1016/0092-8674(87)90240-6. [DOI] [PubMed] [Google Scholar]
  12. Hershey H. P., Barker R. F., Idler K. B., Lissemore J. L., Quail P. H. Analysis of cloned cDNA and genomic sequences for phytochrome: complete amino acid sequences for two gene products expressed in etiolated Avena. Nucleic Acids Res. 1985 Dec 9;13(23):8543–8559. doi: 10.1093/nar/13.23.8543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kay S. A., Keith B., Shinozaki K., Chye M. L., Chua N. H. The rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region. Plant Cell. 1989 Mar;1(3):351–360. doi: 10.1105/tpc.1.3.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kay S. A., Nagatani A., Keith B., Deak M., Furuya M., Chua N. H. Rice Phytochrome Is Biologically Active in Transgenic Tobacco. Plant Cell. 1989 Aug;1(8):775–782. doi: 10.1105/tpc.1.8.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Keller J. M., Shanklin J., Vierstra R. D., Hershey H. P. Expression of a functional monocotyledonous phytochrome in transgenic tobacco. EMBO J. 1989 Apr;8(4):1005–1012. doi: 10.1002/j.1460-2075.1989.tb03467.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Larkins B. A., Hurkman W. J. Synthesis and deposition of zein in protein bodies of maize endosperm. Plant Physiol. 1978 Aug;62(2):256–263. doi: 10.1104/pp.62.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Müllner E. W., Kühn L. C. A stem-loop in the 3' untranslated region mediates iron-dependent regulation of transferrin receptor mRNA stability in the cytoplasm. Cell. 1988 Jun 3;53(5):815–825. doi: 10.1016/0092-8674(88)90098-0. [DOI] [PubMed] [Google Scholar]
  18. Pachter J. S., Yen T. J., Cleveland D. W. Autoregulation of tubulin expression is achieved through specific degradation of polysomal tubulin mRNAs. Cell. 1987 Oct 23;51(2):283–292. doi: 10.1016/0092-8674(87)90155-3. [DOI] [PubMed] [Google Scholar]
  19. Quail P. H., Colbert J. T., Peters N. K., Christensen A. H., Sharrock R. A., Lissemore J. L. Phytochrome and the regulation of the expression of its genes. Philos Trans R Soc Lond B Biol Sci. 1986 Nov 17;314(1166):469–480. doi: 10.1098/rstb.1986.0066. [DOI] [PubMed] [Google Scholar]
  20. Schopfer P. pH-Dependence of Extension Growth in Avena Coleoptiles and Its Implications for the Mechanism of Auxin Action. Plant Physiol. 1989 May;90(1):202–207. doi: 10.1104/pp.90.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shah D. M., Hightower R. C., Meagher R. B. Complete nucleotide sequence of a soybean actin gene. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1022–1026. doi: 10.1073/pnas.79.4.1022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shanklin J., Jabben M., Vierstra R. D. Red light-induced formation of ubiquitin-phytochrome conjugates: Identification of possible intermediates of phytochrome degradation. Proc Natl Acad Sci U S A. 1987 Jan;84(2):359–363. doi: 10.1073/pnas.84.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shapiro D. J., Blume J. E., Nielsen D. A. Regulation of messenger RNA stability in eukaryotic cells. Bioessays. 1987 May;6(5):221–226. doi: 10.1002/bies.950060507. [DOI] [PubMed] [Google Scholar]
  24. Shaw G., Kamen R. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell. 1986 Aug 29;46(5):659–667. doi: 10.1016/0092-8674(86)90341-7. [DOI] [PubMed] [Google Scholar]
  25. Shyu A. B., Belasco J. G., Greenberg M. E. Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay. Genes Dev. 1991 Feb;5(2):221–231. doi: 10.1101/gad.5.2.221. [DOI] [PubMed] [Google Scholar]
  26. Silflow C. D., Key J. L. Stability of polysome-associated polyadenylated RNA from soybean suspension culture cells. Biochemistry. 1979 Mar 20;18(6):1013–1018. doi: 10.1021/bi00573a013. [DOI] [PubMed] [Google Scholar]
  27. Sullivan T. D., Christensen A. H., Quail P. H. Isolation and characterization of a maize chlorophyll a/b binding protein gene that produces high levels of mRNA in the dark. Mol Gen Genet. 1989 Feb;215(3):431–440. doi: 10.1007/BF00427040. [DOI] [PubMed] [Google Scholar]
  28. Taliercio E. W., Chourey P. S. Post-transcriptional control of sucrose synthase expression in anaerobic seedlings of maize. Plant Physiol. 1989 Aug;90(4):1359–1364. doi: 10.1104/pp.90.4.1359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wadsworth G. J., Redinbaugh M. G., Scandalios J. G. A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis. Anal Biochem. 1988 Jul;172(1):279–283. doi: 10.1016/0003-2697(88)90443-5. [DOI] [PubMed] [Google Scholar]
  30. Wilson T., Treisman R. Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences. Nature. 1988 Nov 24;336(6197):396–399. doi: 10.1038/336396a0. [DOI] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES