Skip to main content
PMC home page
The Plant Cell logoLink to The Plant Cell
. 1992 Feb;4(2):129–134. doi: 10.1105/tpc.4.2.129

Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors.

EE Farmer 1, CA Ryan 1
PMCID: PMC160114  PMID: 12297644

Abstract

Jasmonic acid and methyl jasmonate have been shown previously to be powerful inducers of proteinase inhibitors in tomato, tobacco, and alfalfa leaves. We show here that when proposed octadecanoid precursors of jasmonic acid, i.e., linolenic acid, 13(S)-hydroperoxylinolenic acid, and phytodienoic acid, were applied to the surfaces of tomato leaves, these compounds also served as powerful inducers of proteinase inhibitor I and II synthesis, a simulation of a wound response. By contrast, compounds closely related to the precursors but which are not intermediates in the jasmonic acid biosynthetic pathway did not induce proteinase inhibitor synthesis. These results suggest that the octadecanoid intermediates may participate in a lipid-based signaling system that activates proteinase inhibitor synthesis in response to insect and pathogen attack.

Full Text

The Full Text of this article is available as a PDF (502.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop P. D., Makus D. J., Pearce G., Ryan C. A. Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymically released from cell walls. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3536–3540. doi: 10.1073/pnas.78.6.3536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradshaw H. D., Jr, Hollick J. B., Parsons T. J., Clarke H. R., Gordon M. P. Systemically wound-responsive genes in poplar trees encode proteins similar to sweet potato sporamins and legume Kunitz trypsin inhibitors. Plant Mol Biol. 1990 Jan;14(1):51–59. doi: 10.1007/BF00015654. [DOI] [PubMed] [Google Scholar]
  3. Brown W. E., Ryan C. A. Isolation and characterization of a wound-induced trypsin inhibitor from alfalfa leaves. Biochemistry. 1984 Jul 17;23(15):3418–3422. doi: 10.1021/bi00310a006. [DOI] [PubMed] [Google Scholar]
  4. Farmer E. E., Moloshok T. D., Saxton M. J., Ryan C. A. Oligosaccharide signaling in plants. Specificity of oligouronide-enhanced plasma membrane protein phosphorylation. J Biol Chem. 1991 Feb 15;266(5):3140–3145. [PubMed] [Google Scholar]
  5. Farmer E. E., Ryan C. A. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7713–7716. doi: 10.1073/pnas.87.19.7713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kernan A., Thornburg R. W. Auxin Levels Regulate the Expression of a Wound-Inducible Proteinase Inhibitor II-Chloramphenicol Acetyl Transferase Gene Fusion in Vitro and in Vivo. Plant Physiol. 1989 Sep;91(1):73–78. doi: 10.1104/pp.91.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Needleman P., Turk J., Jakschik B. A., Morrison A. R., Lefkowith J. B. Arachidonic acid metabolism. Annu Rev Biochem. 1986;55:69–102. doi: 10.1146/annurev.bi.55.070186.000441. [DOI] [PubMed] [Google Scholar]
  8. Pearce G., Strydom D., Johnson S., Ryan C. A. A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins. Science. 1991 Aug 23;253(5022):895–897. doi: 10.1126/science.253.5022.895. [DOI] [PubMed] [Google Scholar]
  9. Pēna-Cortés H., Sánchez-Serrano J. J., Mertens R., Willmitzer L., Prat S. Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9851–9855. doi: 10.1073/pnas.86.24.9851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ryan C. A. Oligosaccharide signalling in plants. Annu Rev Cell Biol. 1987;3:295–317. doi: 10.1146/annurev.cb.03.110187.001455. [DOI] [PubMed] [Google Scholar]
  11. Ryan C. A. Quantitative determination of soluble cellular proteins by radial diffusion in agar gels containing antibodies. Anal Biochem. 1967 Jun;19(3):434–440. doi: 10.1016/0003-2697(67)90233-3. [DOI] [PubMed] [Google Scholar]
  12. Trautman R., Cowan K. M., Wagner G. G. Data processing for radial immunodiffusion. Immunochemistry. 1971 Oct;8(10):901–916. doi: 10.1016/0019-2791(71)90429-0. [DOI] [PubMed] [Google Scholar]
  13. Vick B. A., Feng P., Zimmerman D. C. Formation of 12-[18-O]oxo-cis-10, cis-15-phytodienoic acid from 13[18-O]hydroperoxylinolenic acid by hydroperoxide cyclase. Lipids. 1980 Jun;15(6):468–471. doi: 10.1007/BF02534074. [DOI] [PubMed] [Google Scholar]
  14. Vick B. A., Zimmerman D. C. Biosynthesis of jasmonic Acid by several plant species. Plant Physiol. 1984 Jun;75(2):458–461. doi: 10.1104/pp.75.2.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Walker-Simmons M., Holländer-Czytko H., Andersen J. K., Ryan C. A. Wound signals in plants: A systemic plant wound signal alters plasma membrane integrity. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3737–3741. doi: 10.1073/pnas.81.12.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES