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. 1992 Feb;4(2):185–192. doi: 10.1105/tpc.4.2.185

The Commelina yellow mottle virus promoter is a strong promoter in vascular and reproductive tissues.

S L Medberry 1, B E Lockhart 1, N E Olszewski 1
PMCID: PMC160119  PMID: 1633493

Abstract

Commelina yellow mottle virus (CoYMV) is a double-stranded DNA virus that infects the monocot Commelina diffusa. Although CoYMV and cauliflower mosaic virus (CaMV; another double-stranded DNA virus) probably replicate by a similar mechanism, the particle morphology and host range of CoYMV place it in a distinct group. We present evidence that a prompter fragment isolated from CoYMV confers a tissue-specific pattern of expression that is different from that conferred by the CaMV 35S promoter. When the CoYMV promoter is used to drive expression of the beta-glucuronidase reporter gene in stably transformed tobacco plants, beta-glucuronidase activity occurs primarily in the phloem, the phloem-associated cells, and the axial parenchyma of roots, stems, leaves, and flowers. Activity is also detected throughout the anther, with highest activity in the tapetum. In contrast, the CaMV 35S promoter is active in most cell types. The CoYMV promoter is a strong promoter, and when the activity of the CoYMV promoter is compared with that of a duplicated CaMV 35S promoter, it is 30% as active in tobacco suspension cells and up to 25% as active in maize suspension cells. These properties of the CoYMV promoter make it potentially useful for high-level expression of engineered genes in vascular cells.

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Selected References

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  1. Benfey P. N., Chua N. H. Regulated genes in transgenic plants. Science. 1989 Apr 14;244(4901):174–181. doi: 10.1126/science.244.4901.174. [DOI] [PubMed] [Google Scholar]
  2. Bonneville J. M., Sanfaçon H., Fütterer J., Hohn T. Posttranscriptional trans-activation in cauliflower mosaic virus. Cell. 1989 Dec 22;59(6):1135–1143. doi: 10.1016/0092-8674(89)90769-1. [DOI] [PubMed] [Google Scholar]
  3. Bouchez D., Tokuhisa J. G., Llewellyn D. J., Dennis E. S., Ellis J. G. The ocs-element is a component of the promoters of several T-DNA and plant viral genes. EMBO J. 1989 Dec 20;8(13):4197–4204. doi: 10.1002/j.1460-2075.1989.tb08605.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Callis J., Fromm M., Walbot V. Introns increase gene expression in cultured maize cells. Genes Dev. 1987 Dec;1(10):1183–1200. doi: 10.1101/gad.1.10.1183. [DOI] [PubMed] [Google Scholar]
  5. Dekeyser R. A., Claes B., De Rycke RMU., Habets M. E., Van Montagu M. C., Caplan A. B. Transient Gene Expression in Intact and Organized Rice Tissues. Plant Cell. 1990 Jul;2(7):591–602. doi: 10.1105/tpc.2.7.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fang R. X., Nagy F., Sivasubramaniam S., Chua N. H. Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants. Plant Cell. 1989 Jan;1(1):141–150. doi: 10.1105/tpc.1.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gordon-Kamm W. J., Spencer T. M., Mangano M. L., Adams T. R., Daines R. J., Start W. G., O'Brien J. V., Chambers S. A., Adams W. R., Jr, Willetts N. G. Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. Plant Cell. 1990 Jul;2(7):603–618. doi: 10.1105/tpc.2.7.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gowda S., Wu F. C., Scholthof H. B., Shepherd R. J. Gene VI of figwort mosaic virus (caulimovirus group) functions in posttranscriptional expression of genes on the full-length RNA transcript. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9203–9207. doi: 10.1073/pnas.86.23.9203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hay J. M., Jones M. C., Blakebrough M. L., Dasgupta I., Davies J. W., Hull R. An analysis of the sequence of an infectious clone of rice tungro bacilliform virus, a plant pararetrovirus. Nucleic Acids Res. 1991 May 25;19(10):2615–2621. doi: 10.1093/nar/19.10.2615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jefferson R. A., Kavanagh T. A., Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. doi: 10.1002/j.1460-2075.1987.tb02730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kay R., Chan A., Daly M., McPherson J. Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes. Science. 1987 Jun 5;236(4806):1299–1302. doi: 10.1126/science.236.4806.1299. [DOI] [PubMed] [Google Scholar]
  12. Kunkel T. A., Roberts J. D., Zakour R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987;154:367–382. doi: 10.1016/0076-6879(87)54085-x. [DOI] [PubMed] [Google Scholar]
  13. Lam E., Benfey P. N., Gilmartin P. M., Fang R. X., Chua N. H. Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7890–7894. doi: 10.1073/pnas.86.20.7890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lam E., Chua N. H. ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters. Plant Cell. 1989 Dec;1(12):1147–1156. doi: 10.1105/tpc.1.12.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lazo G. R., Stein P. A., Ludwig R. A. A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology (N Y) 1991 Oct;9(10):963–967. doi: 10.1038/nbt1091-963. [DOI] [PubMed] [Google Scholar]
  16. Medberry S. L., Lockhart B. E., Olszewski N. E. Properties of Commelina yellow mottle virus's complete DNA sequence, genomic discontinuities and transcript suggest that it is a pararetrovirus. Nucleic Acids Res. 1990 Sep 25;18(18):5505–5513. doi: 10.1093/nar/18.18.5505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ow D. W., Jacobs J. D., Howell S. H. Functional regions of the cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4870–4874. doi: 10.1073/pnas.84.14.4870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pfeiffer P., Hohn T. Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell. 1983 Jul;33(3):781–789. doi: 10.1016/0092-8674(83)90020-x. [DOI] [PubMed] [Google Scholar]
  19. Sanger M., Daubert S., Goodman R. M. Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter. Plant Mol Biol. 1990 Mar;14(3):433–443. doi: 10.1007/BF00028779. [DOI] [PubMed] [Google Scholar]
  20. Tabata T., Nakayama T., Mikami K., Iwabuchi M. HBP-1a and HBP-1b: leucine zipper-type transcription factors of wheat. EMBO J. 1991 Jun;10(6):1459–1467. doi: 10.1002/j.1460-2075.1991.tb07666.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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