Abstract
We monitored Fed-1 (encoding ferredoxin I) mRNA levels in etiolated transgenic tobacco seedlings containing the intact pea Fed-1 gene to determine if the characteristic light responses of this gene in pea seedlings are also observed in transgenic tobacco. Fed-1 transcript levels in transgenic tobacco seedlings closely paralleled those of the native gene in pea buds when etiolated seedlings were transferred to white light. However, the response to red light was much smaller in tobacco than in pea and was not efficiently reversed by far-red light. The red light response of endogenous tobacco ferredoxin transcripts is closely comparable to that of the Fed-1 transgene, with a similar lack of photoreversibility. Thus, the pea Fed-1 transgene responds normally to tobacco gene-regulatory factors, but these factors are less influenced by phytochrome in tobacco cotyledons than in pea buds.
Full Text
The Full Text of this article is available as a PDF (2.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berry-Lowe S. L., Meagher R. B. Transcriptional regulation of a gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean tissue is linked to the phytochrome response. Mol Cell Biol. 1985 Aug;5(8):1910–1917. doi: 10.1128/mcb.5.8.1910. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elliott R. C., Dickey L. F., White M. J., Thompson W. F. cis-Acting Elements for Light Regulation of Pea Ferredoxin I Gene Expression Are Located within Transcribed Sequences. Plant Cell. 1989 Jul;1(7):691–698. doi: 10.1105/tpc.1.7.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elliott R. C., Pedersen T. J., Fristensky B., White M. J., Dickey L. F., Thompson W. F. Characterization of a single copy gene encoding ferredoxin I from pea. Plant Cell. 1989 Jul;1(7):681–690. doi: 10.1105/tpc.1.7.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallo-Meagher M., Sowinski D. A., Elliott R. C., Thompson W. F. Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings. Plant Cell. 1992 Apr;4(4):389–395. doi: 10.1105/tpc.4.4.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L. S., Briggs W. R., Thompson W. F. Phytochrome control of specific mRNA levels in developing pea buds : the presence of both very low fluence and low fluence responses. Plant Physiol. 1985 Jun;78(2):388–393. doi: 10.1104/pp.78.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L. S., Roberts L. L., Briggs W. R., Thompson W. F. Phytochrome Control of Specific mRNA levels in Developing Pea Buds : Kinetics of Accumulation, Reciprocity, and Escape Kinetics of the Low Fluence Response. Plant Physiol. 1986 Aug;81(4):1033–1038. doi: 10.1104/pp.81.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L. S., Thompson W. F., Briggs W. R. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA. Science. 1984 Dec 21;226(4681):1447–1449. doi: 10.1126/science.226.4681.1447. [DOI] [PubMed] [Google Scholar]
- Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mösinger E., Batschauer A., Apel K., Schäfer E., Briggs W. R. Phytochrome regulation of greening in barley : effects on mRNA abundance and on transcriptional activity of isolated nuclei. Plant Physiol. 1988 Mar;86(3):706–710. doi: 10.1104/pp.86.3.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagy F., Kay S. A., Boutry M., Hsu M. Y., Chua N. H. Phytochrome-controlled expression of a wheat Cab gene in transgenic tobacco seedlings. EMBO J. 1986 Jun;5(6):1119–1124. doi: 10.1002/j.1460-2075.1986.tb04335.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wehmeyer B., Cashmore A. R., Schäfer E. Photocontrol of the Expression of Genes Encoding Chlorophyll a/b Binding Proteins and Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase in Etiolated Seedlings of Lycopersicon esculentum (L.) and Nicotiana tabacum (L.). Plant Physiol. 1990 Jul;93(3):990–997. doi: 10.1104/pp.93.3.990. [DOI] [PMC free article] [PubMed] [Google Scholar]