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. 1992 Apr;4(4):389–395. doi: 10.1105/tpc.4.4.389

Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings.

M Gallo-Meagher 1, D A Sowinski 1, R C Elliott 1, W F Thompson 1
PMCID: PMC160139  PMID: 1498599

Abstract

In previous studies using leaves of light-grown transgenic tobacco plants, we have shown that sequences located within the transcribed region of the pea Fed-1 gene (encoding ferredoxin I) are major cis-acting determinants of light-regulated mRNA accumulation. However, we show here that these internal sequences are less important for the Fed-1 light response in etiolated tobacco seedlings than they are in green leaves and that upstream elements confer organ specificity and contribute significantly to Fed-1 light responses in etiolated material. Light effects mediated by upstream response elements are thus most pronounced during the initial induction of gene activity, whereas internal elements play a more prominent role in modulating Fed-1 expression once the gene is already active.

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Selected References

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