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. 1992 Apr;4(4):425–433. doi: 10.1105/tpc.4.4.425

A carrot somatic embryo mutant is rescued by chitinase.

A J De Jong 1, J Cordewener 1, F Lo Schiavo 1, M Terzi 1, J Vandekerckhove 1, A Van Kammen 1, S C De Vries 1
PMCID: PMC160142  PMID: 1498601

Abstract

At the nonpermissive temperature, somatic embryogenesis of the temperature-sensitive (ts) carrot cell mutant ts11 does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-type cells to the culture medium. From this mixture of secreted proteins, a 32-kD glycoprotein, designated extracellular protein 3 (EP3), that allows completion of somatic embryo development in ts11 at the nonpermissive temperature was purified. On the basis of peptide sequences and biochemical characterization, EP3 was identified as a glycosylated acidic endochitinase. The addition of the 32-kD endochitinase to ts11 embryo cultures at the nonpermissive temperature appeared to promote the formation of a correctly formed embryo protoderm. These results imply that a glycosylated acidic endochitinase has an important function in early plant somatic embryo development.

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Selected References

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