Figure 1.
Expression levels of p14ARF in MPM cell lines. RNA was isolated from MPM primary cultures and cell lines and SV40-transformed Met5A cells, and RT-PCR was performed to detect p14ARF, SV40 L-Tag and GAPDH mRNA. Lysates of the same cell lines were analyzed by immunoblotting using anti-p14ARF C-terminal antibodies. Staining of actin was used as equal loading control.