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. 1992 Dec;4(12):1519–1530. doi: 10.1105/tpc.4.12.1519

Initial characterization of a pea mutant with light-independent photomorphogenesis.

S Frances 1, M J White 1, M D Edgerton 1, A M Jones 1, R C Elliott 1, W F Thompson 1
PMCID: PMC160238  PMID: 1467651

Abstract

We have identified a mutant of pea cultivar Alaska that has many of the characteristics normally associated with light-grown seedlings even when grown in complete darkness. We have designated this mutant lip1, for light independent photomorphogenesis. Etiolated wild-type pea seedlings are white to slightly yellow in color and have a distinct morphology characterized by elongated epicotyls and buds containing unexpanded leaves with small, undifferentiated cells. In contrast, mutant seedlings grown under the same conditions are yellow in color and have short epicotyls and expanded leaves showing clear cellular differentiation. Transmission electron microscopy revealed partially developed, agranal plastids in the dark-grown mutant, unlike wild-type seedlings that contain etioplasts with prolamellar bodies. The mutant also exhibits a much shorter lag period for chlorophyll accumulation when etiolated seedlings are transferred from darkness to white light. The dark-grown mutant has 10-fold less spectrally detectable phytochrome, which can be attributed to a 10-fold reduction in the level of the PHYA polypeptide. Cab, Fed1, and RbcS transcripts are present in dark-grown mutant seedlings at levels comparable to those produced in light-grown material. The levels of these transcripts show a normal decrease when green plants grown for 15 days in a light/dark cycle are transferred to continuous darkness. However, transcript levels remain high during dark treatment of seedlings grown for 9 days in continuous light, indicating that the dark adaptation response in this mutant is developmentally plastic. The lip1 mutant has several features in common with the deetiolated Arabidopsis mutants det1, det2, and cop1. However, there are also several important differences, including varying effects on phytochrome levels, organ-specific gene expression, plastid development, and response to dark adaptation.

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Selected References

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  1. Broglie R., Bellemare G., Bartlett S. G., Chua N. H., Cashmore A. R. Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7304–7308. doi: 10.1073/pnas.78.12.7304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cesarone C. F., Bolognesi C., Santi L. Improved microfluorometric DNA determination in biological material using 33258 Hoechst. Anal Biochem. 1979 Nov 15;100(1):188–197. doi: 10.1016/0003-2697(79)90131-3. [DOI] [PubMed] [Google Scholar]
  3. Chory J. Light signals in leaf and chloroplast development: photoreceptors and downstream responses in search of a transduction pathway. New Biol. 1991 Jun;3(6):538–548. [PubMed] [Google Scholar]
  4. Chory J., Nagpal P., Peto C. A. Phenotypic and Genetic Analysis of det2, a New Mutant That Affects Light-Regulated Seedling Development in Arabidopsis. Plant Cell. 1991 May;3(5):445–459. doi: 10.1105/tpc.3.5.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chory J., Peto C. A., Ashbaugh M., Saganich R., Pratt L., Ausubel F. Different Roles for Phytochrome in Etiolated and Green Plants Deduced from Characterization of Arabidopsis thaliana Mutants. Plant Cell. 1989 Sep;1(9):867–880. doi: 10.1105/tpc.1.9.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chory J., Peto C., Feinbaum R., Pratt L., Ausubel F. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell. 1989 Sep 8;58(5):991–999. doi: 10.1016/0092-8674(89)90950-1. [DOI] [PubMed] [Google Scholar]
  7. Cordonnier M. M., Greppin H., Pratt L. H. Identification of a highly conserved domain on phytochrome from angiosperms to algae. Plant Physiol. 1986 Apr;80(4):982–987. doi: 10.1104/pp.80.4.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deng X. W., Caspar T., Quail P. H. cop1: a regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev. 1991 Jul;5(7):1172–1182. doi: 10.1101/gad.5.7.1172. [DOI] [PubMed] [Google Scholar]
  9. Elliott R. C., Pedersen T. J., Fristensky B., White M. J., Dickey L. F., Thompson W. F. Characterization of a single copy gene encoding ferredoxin I from pea. Plant Cell. 1989 Jul;1(7):681–690. doi: 10.1105/tpc.1.7.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gallo-Meagher M., Sowinski D. A., Elliott R. C., Thompson W. F. Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings. Plant Cell. 1992 Apr;4(4):389–395. doi: 10.1105/tpc.4.4.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hirsch A. M., Bang M., Ausubel F. M. Ultrastructural analysis of ineffective alfalfa nodules formed by nif::Tn5 mutants of Rhizobium meliloti. J Bacteriol. 1983 Jul;155(1):367–380. doi: 10.1128/jb.155.1.367-380.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kloppstech K., Otto B., Sierralta W. Cyclic temperature treatments of dark-grown pea seedlings induce a rise in specific transcript levels of light-regulated genes related to photomorphogenesis. Mol Gen Genet. 1991 Mar;225(3):468–473. doi: 10.1007/BF00261689. [DOI] [PubMed] [Google Scholar]
  13. López-Juez E., Nagatani A., Tomizawa K., Deak M., Kern R., Kendrick R. E., Furuya M. The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. Plant Cell. 1992 Mar;4(3):241–251. [PMC free article] [PubMed] [Google Scholar]
  14. Moran R., Porath D. Chlorophyll determination in intact tissues using n,n-dimethylformamide. Plant Physiol. 1980 Mar;65(3):478–479. doi: 10.1104/pp.65.3.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Okada K., Shimura Y. Aspects of recent developments in mutational studies of plant signaling pathways. Cell. 1992 Aug 7;70(3):369–372. doi: 10.1016/0092-8674(92)90159-a. [DOI] [PubMed] [Google Scholar]
  16. Parks B. M., Quail P. H. Phytochrome-Deficient hy1 and hy2 Long Hypocotyl Mutants of Arabidopsis Are Defective in Phytochrome Chromophore Biosynthesis. Plant Cell. 1991 Nov;3(11):1177–1186. doi: 10.1105/tpc.3.11.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
  18. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomizawa K., Nayatani A., Furuya M. Phytochrome genes: studies using the tools of molecular biology and photomorphogenetic mutants. Photochem Photobiol. 1990 Jul;52(1):265–275. doi: 10.1111/j.1751-1097.1990.tb01784.x. [DOI] [PubMed] [Google Scholar]
  20. Wehmeyer B., Cashmore A. R., Schäfer E. Photocontrol of the Expression of Genes Encoding Chlorophyll a/b Binding Proteins and Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase in Etiolated Seedlings of Lycopersicon esculentum (L.) and Nicotiana tabacum (L.). Plant Physiol. 1990 Jul;93(3):990–997. doi: 10.1104/pp.93.3.990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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