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. 1993 Jan;5(1):75–86. doi: 10.1105/tpc.5.1.75

Expression of a self-incompatibility gene in a self-compatible line of Brassica oleracea.

T Gaude 1, A Friry 1, P Heizmann 1, C Mariac 1, M Rougier 1, I Fobis 1, C Dumas 1
PMCID: PMC160252  PMID: 8439745

Abstract

In cruciferous plants, self-pollination is prevented by the action of genes situated at the self-incompatibility locus or S-locus. The self-incompatibility reaction is associated with expression of stigma glycoproteins encoded by the S-locus glycoprotein (SLG) gene. Only a few cases of self-compatible plants derived from self-incompatible lines in the crucifer Brassica have been reported. In these cases, self-compatibility was generally ascribed to the action of single genes unlinked to the S-locus. In contrast, we report here a line of Brassica oleracea var acephala with a self-compatible phenotype linked to the S-locus. By means of both biochemical and immunochemical analyses, we showed that this self-compatible (Sc) line nonetheless possesses stigmatic SLGs (SLG-Sc) that are expressed with a similar spatial and temporal pattern to that described for the SLGs of self-incompatible Brassica plants. Moreover, the SLG-Sc products segregate with the self-compatibility phenotype in F2 progeny, suggesting that changes at the S-locus may be responsible for the occurrence of the self-compatibility character. A cDNA clone encoding the SLG-Sc product was isolated, and the deduced amino acid sequence showed this glycoprotein to be highly homologous to the pollen recessive S2 allele glycoprotein. Hence, self-compatibility in this Brassica Sc line correlates with the expression of a pollen recessive-like S allele in the stigma.

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Selected References

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  1. Boyes D. C., Chen C. H., Tantikanjana T., Esch J. J., Nasrallah J. B. Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci. Genetics. 1991 Jan;127(1):221–228. doi: 10.1093/genetics/127.1.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen C. H., Nasrallah J. B. A new class of S sequences defined by a pollen recessive self-incompatibility allele of Brassica oleracea. Mol Gen Genet. 1990 Jul;222(2-3):241–248. doi: 10.1007/BF00633824. [DOI] [PubMed] [Google Scholar]
  3. Dickinson H. G., Crabbe M. J., Gaude T. Sporophytic self-incompatibility systems: S gene products. Int Rev Cytol. 1992;140:525–561. doi: 10.1016/s0074-7696(08)61108-0. [DOI] [PubMed] [Google Scholar]
  4. Gaude T., Denoroy L., Dumas C. Use of a fast protein electrophoretic purification procedure for N-terminal sequence analysis to identify S-locus related proteins in stigmas of Brassica oleracea. Electrophoresis. 1991 Sep;12(9):646–653. doi: 10.1002/elps.1150120909. [DOI] [PubMed] [Google Scholar]
  5. Kandasamy M. K., Paolillo D. J., Faraday C. D., Nasrallah J. B., Nasrallah M. E. The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae. Dev Biol. 1989 Aug;134(2):462–472. doi: 10.1016/0012-1606(89)90119-x. [DOI] [PubMed] [Google Scholar]
  6. Lalonde B. A., Nasrallah M. E., Dwyer K. G., Chen C. H., Barlow B., Nasrallah J. B. A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene. Plant Cell. 1989 Feb;1(2):249–258. doi: 10.1105/tpc.1.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lane R. D., Crissman R. S., Ginn S. High efficiency fusion procedure for producing monoclonal antibodies against weak immunogens. Methods Enzymol. 1986;121:183–192. doi: 10.1016/0076-6879(86)21017-4. [DOI] [PubMed] [Google Scholar]
  8. MARTIN F. W. Staining and observing pollen tubes in the style by means of fluorescence. Stain Technol. 1959 May;34(3):125–128. doi: 10.3109/10520295909114663. [DOI] [PubMed] [Google Scholar]
  9. Nasrallah J. B., Yu S. M., Nasrallah M. E. Self-incompatibility genes of Brassica oleracea: Expression, isolation, and structure. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5551–5555. doi: 10.1073/pnas.85.15.5551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Scutt C. P., Croy R. R. An S5 self-incompatibility allele-specific cDNA sequence from Brassica oleracea shows high homology to the SLR2 gene. Mol Gen Genet. 1992 Mar;232(2):240–246. doi: 10.1007/BF00280002. [DOI] [PubMed] [Google Scholar]
  11. Stein J. C., Howlett B., Boyes D. C., Nasrallah M. E., Nasrallah J. B. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816–8820. doi: 10.1073/pnas.88.19.8816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Trick M., Heizmann P. Sporophytic self-incompatibility systems: Brassica S gene family. Int Rev Cytol. 1992;140:485–524. doi: 10.1016/s0074-7696(08)61107-9. [DOI] [PubMed] [Google Scholar]
  13. Umbach A. L., Lalonde B. A., Kandasamy M. K., Nasrallah J. B., Nasrallah M. E. Immunodetection of protein glycoforms encoded by two independent genes of the self-incompatibility multigene family of brassica. Plant Physiol. 1990 Jun;93(2):739–747. doi: 10.1104/pp.93.2.739. [DOI] [PMC free article] [PubMed] [Google Scholar]

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